Three new water-soluble alkaloids from the leaves of Suregada glomerulata (Blume) Baill

Three new water-soluble alkaloids (1-3) together with twelve known compounds (4-15) have been isolated from the water extract of leaves of Suregada glomerulata. Their structures were determined by spectroscopic analysis and chemical method. Compounds 1-3 were evaluated for their in vitro inhibitory activity against α-glucosidase and HIV-1 replication. However, no significant activities were found.     

华山松松塔提取物抗HIV活性部位中铅含量的测定

[目的]为了分析华山松松塔提取物抗HIV活性部位中铅含量。[方法]应用石墨炉原子吸收光谱法,参照中华人民共和国药典2010版[附录ⅨB铅、镉、砷、汞、铜测定法(一部)]测定活性部位中铅含量。[结果]活性部位中铅含量在中华人民共和国对外经济贸易合作部《药用植物及制剂进出口绿色行业标准》和《中国药典》规定范围(Pb≤5.0 mg/kg)之内,没有超标。[结论]该方法简单、方便、快速、灵敏,为抗HIV活性部位重金属铅含量测试提供一种可靠的方法。     

岩藻聚糖硫酸酯的生物活性及作用机理研究进展

岩藻聚糖硫酸酯（Fucoidan）是一种含有硫酸酯的水溶性杂聚糖,是褐藻中特有的一种化学组分。研究发现岩藻聚糖硫酸酯在医学方面具有独特的功效,具有抗凝血、抗肿瘤、抗HIV、抗血栓、提高免疫力、降血脂等活性。综述了岩藻聚糖硫酸酯近几年在生物活性方面的研究进展。     

vMIP-Ⅰ激活Jurkat细胞抗-HIV基因表达的研究

本研究通过构建真核表达载体pEGFP-N3-vMIP-Ⅰ,电穿孔法将其转染至Jurkat细胞,荧光定量PCR检测vMIP-Ⅰ基因对Jurkat细胞内CCL5、APOBEC3G、APOBEC3F、等抗-HIV基因表达水平的影响,从而探讨vMIP-Ⅰ抗HIV感染的机制。结果显示：成功构建了pEGFP-N3-vMIP-Ⅰ载体,电穿孔转染效率达到40%左右,与转染空载体组相比,vMIP-Ⅰ转染组的Jurkat细胞内CCL5、A3G、A3F和MX1分别上调7.37倍、1.58倍、2.42倍和2.06倍。研究结果表明：vMIP-Ⅰ基因可激活Jurkat细胞内一些抗HIV相关基因的表达,这可能是vMIP-Ⅰ基因抗HIV感染的机制之一。     

Cloning, expression, and characterization of a novel anti-HIV lectin from the cultured cyanobacterium, Oscillatoria agardhii

OAA, the potent anti-HIV protein from Oscillatoria agardhii NIES-204 belongs to a new lectin family, shows strict binding specificity for high-mannose N-glycans, and has an extremely high association constant in the picomolar range for recombinant gp120, an envelope protein of HIV. In this study we have cloned the gene encoding OAA from the genomic DNA of the cyanobacterium, and efficiently expressed the recombinant lectin (rOAA) in Escherichia coli. The rOAA expressed as a His-tagged fusion protein was recovered in a soluble form and purified in high yield (48 mg/1 l-culture) by metal chelate chromatography. The fusion protein was cleaved with factor Xa, and the resulting rOAA was isolated in a final yield of 14.8 mg/1 l-culture by reversed-phase HPLC. Both the N-terminal sequence and the molecular mass of rOAA were found to be identical with those of OAA. The rOAA was fully functional with the same properties as OAA, as evidenced by hemagglutination activity, hapten-inhibition test, and binding specificity for high-mannose-type N-glycans. This rOAA should be applicable as a specific probe for high-mannose N-glycans and should contribute to elucidation of the molecular basis of its strict carbohydrate-binding specificity and potent anti-HIV activity.