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本文以黑胡椒粉为原料,采用三相分离法(TPP)提取胡椒油树脂,研究了胡椒粉添加量、硫酸铵质量浓度、提取液与叔丁醇体积比、pH、温度对胡椒油树脂得率及胡椒碱含量的影响,并通过响应面试验优化了胡椒油树脂的提取工艺。结果表明:最佳提取条件为胡椒粉添加量5%、硫酸铵质量浓度10%、提取液与叔丁醇的体积比1∶0.5、pH 4、温度20℃,在此条件下,黑胡椒油树脂得率为12.90%,黑胡椒油树脂中胡椒碱含量为30.46%,验证试验与模型预测值基本相符。研究结果为工业化提取胡椒油树脂提供参考。 相似文献
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蝙蝠葛碱和胡椒碱对两株鼻咽癌细胞增殖的影响 总被引:9,自引:0,他引:9
目的:观察蝙蝠葛碱和胡椒碱对两株鼻咽癌细胞增殖的影响。方法:用MTT法测定两种药物分别处理CNE-1、CNE-2Z两株细胞的IC5值随作用时间的延长而减少(P<0.01),两种药物处理CNE-1细胞24、48h的IC50值均明显低于CNE-2Z细胞(P<0.01),蝙蝠葛碱处理两株细胞72h的IC50值差异显著(P<0.05)。结论:蝙蝠葛碱和胡椒碱对CNE-1、CNE-2Z细胞株的生长增殖呈时间依赖性抑制,对CNE-1细胞株的增殖抑制作用明显强于CNE-2Z细胞株。 相似文献
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Vanderlúcia F de Paula Luiz C de A Barbosa Antnio J Demuner Dorila Pil‐Veloso Marcelo C Picano 《Pest management science》2000,56(2):168-174
The natural lipophilic amides piperine and piperiline were isolated from Piper nigrum L (Piperaceae). Piperine was hydrolysed into piperic acid (85% yield) which was converted into 16 amides (28–89% yield). The contact toxicity of all synthetic amides, and also that of piperine and piperiline, at the dose 10 µg per insect, was evaluated for the Brazilian economically important insects Ascia monuste orseis Latr, Acanthoscelides obtectus Say, Brevicoryne brassicae L, Protopolybia exigua DeSaus and Cornitermes cumulans Kollar. The results demontrated that the insects have different sensivities to the various amides, with mortality ranging from 0 to 97.5% according to the compound and insect species. © 2000 Society of Chemical Industry 相似文献
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介绍了胡椒碱的研究概况及生物学功能,综述了胡椒碱的常规萃取、超临界萃取,以及检测方法方面的研究进展。同时对胡椒碱的应用前景进行了展望。 相似文献
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