粪肠球菌生长曲线的测定及其对小鼠脑组织的影响

为测定致羔羊脑炎粪肠球菌（Enterococcus faecalis）的生长曲线，寻求一种快速而准确的方法测定不同生长时期粪肠球菌数量，并客观评价其毒性强弱及其对小鼠脑组织的影响，试验采用平板菌落计数法和OD-Monitor振荡比浊法（D_λ值法）测定粪肠球菌的生长曲线，探究该菌在合适时间段内的吸光值（D_{600 nm}）与平板菌落计数法测定的活菌数（CFU）的关系。用粪肠球菌感染小鼠，观察记录小鼠的死亡情况，最后采用Karber法计算粪肠球菌感染小鼠的半数致死量（LD₅₀）。用LD₅₀的剂量感染小鼠，及时采集死亡小鼠脑组织，未死亡的小鼠72 h后全部剖杀取脑组织，一部分做涂片染色，制作病理切片，观察病理变化；一部分进行培养，用于PCR方法进行细菌的回收鉴定。结果显示，用两种方法测定此株粪肠球菌的生长曲线基本一致，在2~8 h生长迅速，为对数生长期，8~14 h生长缓慢，为稳定期，14 h之后死亡数增加，进入衰亡期；对12 h粪肠球菌D_{600 nm}与CFU的关系进行探讨，成功建立回归方程：y=20.769x-1.3422，R²=0.997；其感染小鼠的LD₅₀为7.77×10¹¹个活菌。以此剂量感染小鼠，脑组织涂片染色和培养染色，均能看到革兰氏阳性球菌；PCR结果显示，均出现了大小为112 bp的条带。对脑组织进行病理学观察发现该菌可导致脑组织充血、出血、形成微血栓，脑膜充血。通过生长曲线和其D_{600 nm}与CFU关系的建立，可实时监测粪肠球菌数量，为后期更深入研究粪肠球菌穿越血脑屏障的机制奠定重要的理论基础。     

降低人造板热压机液压系统油液污染的探讨

车间粉尘与湖湿空气是人造板热压机液压系统油液外界污染的主要原因，通过构筑封闭式泵房，增设辅助油箱，改变没箱底部结构，设置排污，排水装置，可有效降低人造板热压机液压系统油液污染，改善液压系统性能，提高压机工作效率。     

人类活动对象山湾滩涂沉积物理化特征的累积效应

[目的]研究人类活动对象山湾滩涂沉积物理化特征的累积效应。[方法]选取象山湾沿岸滩涂沉积物作为供试材料,测定沉积物样品粒度特征、磁化率、总有机碳及硝态氮含量。[结果]象山湾滩涂沉积物粒径组成以粉粒为主,质地较为黏重,可塑性和保水性较好,透气性差,且沉积物粒径组成随深度变化明显;磁性物质的含量随着取样深度的增加而不断减少;有机物质含量较高,且随着取样深度的增加,有机质含量明显下降,但总体维持在较高水平;硝态氮含量随取样深度的增加总体呈上升趋势,波动明显。[结论]人类活动对象山湾滩涂影响较为明显,理化特征的累积效应在剖面上表现显著。     

中国对美直接投资的产业选择研究

对于投资国来说，对外直接投资的产业选择对投资收益和潜在收益有较大影响。为了研究中国对美直接投资的产业选择对中国产业的影响，本文基于比较优势，构建发展中国家OFDI决策模型，通过构造评价指标以及构建回归模型，并利用2005-2014年中国对外直接投资数据、出口数据、行业相关数据对中国对美直接投资的效应进行了实证分析。结果表明，作为中国对美直接投资的两个重点产业，第二、第三产业可以为提高本国对应产业的比较优势提供支撑；中国对美直接投资、美国自身的产业比较优势、其他国家对中国直接投资、中国国内的固定资产投资、中国各行业从业人员数均有助于提高中国产业比较优势；由于中国内部约束和外部约束的原因，中国对美直接投资对提升中国产业比较优势的作用还不明显。     

土地规模化经营对农业生态效率影响及城乡市场分割调节作用探析

土地规模化经营是农业生态效率改善的重要动力,但土地规模化经营作用农业生态效率受到城乡市场分割的门槛调节。该研究借助2001—2017年全国30个地区(因数据缺失,西藏及港澳台地区未涵盖,下同)面板数据,运用面板门槛模型,就城乡市场分割调节土地规模化经营作用农业生态效率过程展开研究。结果表明:(1)我国农业生态效率总体向好,但地区间差异悬殊;(2)土地规模化经营驱动农业生态效率改善,但存在城乡市场分割门槛调节,弱市场分割地区土地规模化经营促进农业生态效率,强市场分割地区作用相反;(3)城乡市场分割调节土地规模化经营作用农业生态效率主要通过限制农产品、劳动力、资本城乡合理流动,削弱农业新型经营主体绿色农业发展意愿和能力途径展开;(4)我国多数地区城乡市场分割水平偏高,区域发展中城市资源偏向和工业优先发展突出,需进行及时改革。该研究可为政府制定绿色农业发展策略提供参考依据。     

滩涂物权制度的困境及出路

滩涂所有权客体的边界不清晰；可设立滩涂用益物权的范围也不明晰；设立滩涂用益物权时存在与水资源、水生野生动植物利用关系如何理顺的问题。湿地不仅是环境与资源法上的概念，也应进入民法领域。将滩涂作为湿地，能划清滩涂所有权客体的边界，解决滩涂用益物权设立时的困境。生态系统角度下的湿地概念，包含一定的水资源，对水体深度有明确要求，水生野生动植物是其内在的组成部分。将湿地概念纳入民法，能解决这些困境。对湿地奉行保护优先的原则，使得只有在未纳入保护地的滩涂上才能设立用益物权，划清了可设立滩涂用益物权的边界。     

Cause and Consequence in Maize Planting Dates in Germany

An important part of agricultural adaptation is the timing of crop sowing dates, affecting yields and the level of risk incurred during a particular season. Cold stress is especially relevant in maize, Zea mays L., so that the timing of planting in the spring is a tactical response to short‐term weather, but is also subject to strategic planning with regard to longer‐term climate. Both factors compare the potential implications of cold stress to the additional yield obtainable through earlier planting. New cultivars suited to growing conditions in Europe and generally increasing spring temperatures have enabled earlier planting, but it is still dependent on short‐term weather during the planting period. In the context of field‐level decision‐making, a panel regression is used to estimate the relationship between weekly local temperature and precipitation and planting dates at specific sites throughout Germany. Next, localised weather data and planting behaviour are linked to yields at the district (Landkreis) level to show the effects of planting date on yield. Based on these relationships optimal planting dates are explored with some associated costs and benefits. Results show a trend towards earlier planting that follows observed increasing spring temperatures and the availability of more cold‐tolerant cultivars but this advance is buffered by the increasing severity of minimum temperatures during a critical period. Earlier planting potentially increases yield but this is offset by additional management costs and risk. A robust and simple depiction of farmer behaviour in climatic, technological and economic context can help to understand trends in crop management and productivity that effect agricultural landscapes.     

人造板弹性模量测量不确定度的评定

按照JJF—1059—1999《测量不确定度评定与表示》的方法,对中密度纤维板弹性模量测量试验中影响其测量结果不确定度进行了分析和评定。     

灰关联决策在水质面板数据处理中的扩展及应用

针对面板数据灰色关联决策分析评价问题,在经典灰色关联分析和灰色综合评价方法的基础上,构建面板数据灰色关联决策评价模型。将灰色关联决策方法扩展到面板数据分析中,解决了小样本面板数据的灰色评价分析问题。将这种方法应用于水环境面板数据质量评价中,经实例计算验证了面板数据灰色关联决策评价模型的稳定性、合理性和实用性,为水环境面板数据质量分析评价提供了可行的计算思路和方法。     

Accuracy of imputation of single nucleotide polymorphism marker genotypes from low‐density panels in Japanese Black cattle

Using target and reference fattened steer populations, the performance of genotype imputation using lower‐density marker panels in Japanese Black cattle was evaluated. Population imputation was performed using BEAGLE software. Genotype information for approximately 40 000 single nucleotide polymorphism (SNP) markers by Illumina BovineSNP50 BeadChip was available, and imputation accuracy was assessed based on the average concordance rates of the genotypes, varying equally spaced SNP densities, and the number of individuals in the reference population. Two additional statistics were also calculated as indicators of imputation performance. The concordance rates tended to be lower for SNPs with greater minor allele frequencies, or those located near the ends of the chromosomes. Longer autosomes yielded greater imputation accuracies than shorter ones. When SNPs were selected based on linkage disequilibrium information, relative imputation accuracy was slightly improved. When 3000 and 10 000 equally spaced SNPs were used, the imputation accuracies were greater than 90% and approximately 97%, respectively. These results indicate that combining genotyping using a lower‐density SNP chip with genotype imputation based on a population of individuals genotyped using a higher‐density SNP chip is a cost‐effective and valid approach for genomic prediction.