红苋R104种子谷蛋白亚基组成及二硫键连接蛋白研究

应用还原和非还原单向ＳＤＳ－ＰＡＧＥ及二者结合的双向电泳技术，研究了红苋Ｒ１０４种子谷蛋白的亚基组成。谷蛋白由多种亚基组成，高分子量二硫键连接蛋白占其多数，经还原裂解成Ａ（５４ＫＤ）、Ｂ（４０ＫＤ，３９ＫＤ）、Ｃ（３３ＫＤ，３１ＫＤ）、Ｄ（２２ＫＤ，２０ＫＤ，１８ＫＤ，）和Ｅ（１５ＫＤ）５组主要单肽链单元，提出了二硫键连接蛋白的组成模式，并发现低分子量谷蛋白亚基存在肽内二硫键     

Disulphide structure of high-molecular-weight (HMW-) gliadins as affected by terminators

This study aimed at elucidating SS-bonds of HMW-gliadins (HGL) from wheat with the focus on terminators of glutenin polymerisation. HGL from wheat flour extracts non-treated or treated with the S-alkylation reagent N-ethylmaleinimide (NEMI) were compared. HGL from wheat flour Akteur were isolated, hydrolysed with thermolysin and the resulting peptides pre-separated by gel permeation chromatography and analysed by liquid chromatography/mass-spectrometry using alternating electron transfer dissociation/collision-induced dissociation. Altogether, 22 and 28 SS-peptides from samples without and with NEMI treatment, respectively, were identified. Twenty-six peptides included standard SS-bonds of α- and γ-gliadins, high-molecular-weight and low-molecular-weight glutenin subunits. Eleven SS-bonds were identified for the first time. Fifteen peptides unique to HGL contained cysteine residues from gliadins with an odd number of cysteines (ω5-, α- and γ-gliadins). Thus, gliadins with an odd number of cysteines, glutathione and cysteine had acted as terminators of glutenin polymerisation. Decisive differences between samples without and with NEMI treatment were not obvious showing that the termination of polymerisation was already completed in the flour. The two HGL samples, however, were different in the majority of ten peptides that included disulphide-linked low-molecular-weight (LMW) thiols such as glutathione and cysteine with the former being enriched in the non-treated HGL-sample.     

Identification of a Group of Novel y-Gliadin Genes

γ-Gliadins are an important component of wheat seed storage proteins. Four novel γ-gliadin genes （Gli-ngl to Gli-ng4） were cloned from wheat （Triticum aestivum） and Aegilops species. The novel γ-gliadins were much smaller in molecular size when compared to the typical γ-gliadins, which was caused by deletion of the non-repetitive domain, glutamine-rich region, 3＂ part of the repetitive domain, and 5＇ part of the C-terminal, possibly due to illegitimate recombination between the repetitive domain and the C-terminal. As a result, Gli-ngl and Gli-ng4 only contained two and three cysteine residues, respectively. Gli-ngl, as the representative of novel γ-gliadin genes, has been sub-cloned into an Escherichia coli expression system. SDS- PAGE indicated that the both cysteine residues of Gli-ngl could participate in the formation of intermolecular disulphide bonds in vitro. Successful cloning of Gli-ngl from seed cDNA of T. aestivum cv. Chinese Spring suggested that these novel γ-gliadin genes were normally transcribed during the development of seeds. Phylogenic analysis indicated that the four novel γ-gliadin genes had a closer relationship with those from the B （S） genome of wheat.     

Fundamental evaluation of the impact of high Hydrostatic Pressure on oat batters

The use of grains alternative to wheat or rye is a challenging task for cereal technologists, and currently new technologies are under investigation as tools to improve the performances of these alternative grains. In this work the effects of high Hydrostatic Pressure (HP) on oat batters were investigated. Oat batters were treated for 10 min at 200, 300, 350, 400 or 500 MPa. Scanning electron microscopy and bright field microscopy showed that high HP significantly affected oat batter microstructure, and both starch and proteins were affected. Treatment at high HP significantly improved batter viscosity and elasticity. At pressures ≤300 MPa the increase in the viscous component was higher than the increase in the elastic component. On the contrary, at pressures ≥350 MPa the elastic component was predominant. Differential scanning calorimetry revealed that high HP induced starch gelatinisation, which started at 300 MPa and was almost complete after treatment at 500 MPa. High HP also affected water- and salt-soluble as well as urea-soluble oat proteins. Analysis of proteins soluble in different buffers revealed that pressures ≥300 MPa induced the formation of urea-insoluble complexes and/or disulfide bonds. Overall, the extent of starch gelatinisation and protein modification was dependent on the applied pressure, but the results collected so far clearly show that high HP can be used to improve the functionality of oat batters.     

Purification and Characterisation of HighMrGlutenin Subunit 20 and its Linked y-type Subunit from Durum Wheat

HighM_rglutenin subunit 20 and its linked y-type subunit, present in the durum wheat cultivar Lira, were purified by preparative reversed-phase high-performance liquid chromatography (RP–HPLC). Amino acid and N-terminal sequence analysis of subunit 20y confirmed that it corresponded to a y-type subunit. Moreover, the number and position of the cysteine residues in subunit 20 were determined by alkylation with the fluorogenic reagent 7-fluoro-4-sulfamoyl-2,1,3,-benzoxadiazole (ABD-F) and subsequent enzymic digestion with trypsin. N-terminal amino acid sequence analysis of the fluorescent peptides showed that subunit 20 had only two cysteine residues, one in the N-terminal region and the other in the C-terminal domain.     

Chemical Soil Fumigation for Strawberry Nursery in Jalisco (Mexico): 2013–2014 Results of Semarnat/Unido Project

ABSTRACT

We evaluated the effectiveness of alternative fumigants on weed control and plant productivity in two nurseries located at high elevation and low latitude (Ciudad Guzman, Jalisco), during 2013 and 2014 at Driscoll’s and Lassen Canyon South nurseries. Treatments were: MB:chloropicrin (MB:CP); 1,3-dichloropropene:CP; CP alone; metam sodium (MS) alone; dimethyl disulphide (DMDS):CP; and sequentially applied CP and MS. A rotary spading machine was used for MS and CP followed by MS. All treatments produced similar pest suppression and runner-plant yields during 2013 and 2014. However, CP alone showed very poor control of the most abundant weed (barnyardgrass, Echinochloa crus-galli). Two years of work on MB alternatives were not sufficient to provide reliable recommendations on this critical need, therefore, the Methyl Bromide Technical Options Committee recommended critical use nomination for MB in 2015.     

The essential mechanics of capillary crumbling of structured agricultural soils

In soil loosening processes like seedbed preparation, significant soil crumbling is often desired. A better understanding of the mechanics of crumbling is necessary to optimize crumbling operations, particularly in structured agricultural soils in which capillary bonds are dominant. To this end, a model of the mechanics of capillary crumbling in structured agricultural soils is developed. Four sets of experiments on cylindrical soil samples were carried out to investigate the validity of the model and soil crumbling characteristics as influenced by freezing, thawing and drying. After preparation and sample pre-treatment, the samples were dried to different moisture contents and then tested to determine soil bonding strength. Soil water suction was also monitored at testing as were sample dimensions at different stages of experimentation. The model was found to account satisfactorily for the mechanics of capillary crumbling in structured agricultural soils. Freezing had the effect of reducing the strength of inter-aggregate bonds whilst preserving the integrity of soil aggregates during crumbling.     

Shear fracture characterization of green-glued polyurethane wood adhesive bonds at various moisture and gluing conditions

Abstract

The shear fracture properties of green-glued one-component polyurethane (PUR) wood adhesive bonds subjected to kiln drying were investigated. The local shear strength and fracture energy of the wood adhesive bonds were determined from experimentally recorded complete shear stress versus deformation curves of the bond line. A stable test set-up and small specimens that were anti-symmetrically loaded were used in order to get a uniform and pure state of shear stress. Different moisture contents (MCs) and pressing times were investigated. The fracture properties of conventionally dry-glued wood adhesive bonds and of solid wood were used as reference. The results show that the fracture energy of green-glued bonds with PUR adhesive is dependent on the MC of wood and on the pressing time. The same fracture energy and strength can be obtained by green gluing as by dry gluing, but there seems to exist a maximum MC of sapwood, in the range between 78% and 160%, and a minimum pressing time, in the range between 3 h and 48 h, for which it can be achieved. Both dry- and green-glued polyurethane adhesive bonds were more ductile than solid wood.     

Green bonds in the emerging multilateral development banks: A (sufficient) alternative to catalysing private capital?

The article aims at discussing the rise of the green bonds in the context of the multilateral development banks, by problematizing their threats and potentialities to be an alternative to the existing financing system within the sustainable development agenda. It scrutinises the development rhetoric behind the emergence of multilateral development banks in the Global South, specifically in the Asian context, and attempts to foster alternatives to the Bretton Woods institutions. The two largest initiatives in that sense – the New Development Bank and the Asian Infrastructure Investment Bank – started in Asia and also target countries in the region, giving the growing influence of China and the enhancement of interregional cooperation through the BRICS countries, currently led by China, India and Russia. The paper argues that there is limited scope for the promised innovations in both project assessment and the role of private capital, given the prioritisation of infrastructure as the premise to achieving the right to development among emerging and least‐developed economies. The research departed from the quantitative and qualitative analysis of the projects funded by the New Development Bank and the Asian Infrastructure Investment Bank, from their inception until 2018, considering the approach that such institutions have taken to the social and environmental standards from their normative framework.