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Larissa Alves Guimar?es Paula Christine Jimenez Thiciana da Silva Sousa Hozana Patrícia S. Freitas Danilo Damasceno Rocha Diego Veras Wilke Jesús Martín Fernando Reyes Otília Deusdênia Loiola Pessoa Letícia Veras Costa-Lotufo 《Marine drugs》2014,12(12):5839-5855
The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. The cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G0/G1 phase of the cell cycle, with consequent reduction of S and G2/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins. 相似文献
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Fluorochrome staining with chromomycin A3(CMA) was used to characterize and compare the CMA banding patterns of chromosomes in 17 accessions of mandarins (Citrus spp.). All accessions had 2n = 18 chromosomes. These chromosomes were classified into six types based on the number and position
of CMA positive bands; A: two telomeric and one proximal band, B: one telomeric and one proximal band, C: two telomeric bands,
D: one telomeric band, E: without bands, F: one proximal band. Each accession possessed two to five types of chromosomes,
and showed unique CMA banding patterns. Type D and E chromosomes were predominant in all accessions. Their numbers were generally
higher in Chinese and Indian mandarins. Type F chromosome was present only in some mandarins originating in Japan, which could
thus be distinguished from mandarins originating in other areas. This study is the first report of type F chromosome in citrus.
The Vietnamese mandarins we studied were the only one shaving type A, B, C, D and E chromosomes.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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