Association of TNF-α, IL-10 and IL-6 promoter polymorphisms in pulmonary tuberculosis patients and their household contacts of younger age group

Single nucleotide polymorphisms (SNPs) of cytokine genes have been found to be involved in the clinical outcome of Tuberculosis. The present study was aimed to identify the high risk genotypes in Tuberculosis patients and their household contacts. A total of 490 subjects were studied which includes 150 active pulmonary tuberculosis patients (APTB), 190 household contacts (HHC) and 150 healthy controls (HC). The SNPs of TNF-α (-308A/G), IL-10(-1082G/A) and IL-6(-174G/C) were performed by ARMs PCR. The IL-10 GA genotype showed significant association in APTB and HHC and was 2.3 times higher risk in APTB and 3.7 times in HHC compared to HCs. The A allele was found to be significantly associated with the risk of disease. The CC genotype of IL-6 was found to be significantly associated in APTB and an insignificant positive association in HHCs. The multifactor dimensionality reduction (MDR) analysis indicated that the genotypes of IL-6 were showing high risk with GA genotype of IL-10. In conclusion the gene interaction may be useful for identification of genotypes as biomarkers to distinguish high risk individuals.     

苹果果皮细胞膜结构变化与虎皮病的关系

国光苹果在贮藏过程中,果皮内α—法尼烯不断积累,60天左右达最大值,后随共轭三烯的迅速增加而下降。与此同时,果皮中丙二醛积累,组织相对电导率增加。抗氧化剂二苯胺能显著降低果皮中丙二醛积累,阻止相对电导率的上升,减少发病率。对膜结构变化与虎皮病的关系进行了讨论。     

Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.     

非奇异H-矩阵的判定

运用矩阵分析方法,讨论了非奇异H-矩阵的判定问题,得到两个非奇异H-矩阵新的判定准则,并以数值例子说明判定方法的有效性.     

成年牦牛皮肤中血管与神经的组织学观察及HIF-1α在皮肤中的表达

本研究旨在探讨牦牛不同部位皮肤内血管和神经的分布情况,及检测低氧诱导因子-1α（HIF-1α）在不同部位皮肤上的定位及相对表达量,探究牦牛皮肤对高原低氧环境的适应机制。采用HE、Masson’s三色和Verhoeff VG染色法,对成年牦牛皮肤内血管和神经结构进行观察与分析;采用免疫组织化学、实时荧光定量PCR和蛋白免疫印迹法,对HIF-1α的mRNA和蛋白在成年牦牛皮肤组织中表达与分布进行研究。结果表明,颈部血管与神经密度最高,前臂部和小腿部次之,跖部最低,部位间差异显著（P<0.05）。HIF-1α主要表达在表皮层、毛囊的上皮根鞘、皮脂腺、汗腺、血管、神经;颈部、前臂部和小腿部强阳性表达,跖部阳性表达。HIF-1α mRNA的相对表达量跖部明显低于其他部位（P<0.05）,其他三个部位两两比较差异不显著（P>0.05）。HIF-1α蛋白相对表达量颈部最高,跖部最低,差异显著（P<0.05）。研究结果提示,成年牦牛不同部位皮肤内不同血管和神经形态结构相似,密度从颈部到前肢再到后肢差异显著。HIF-1α的差异性表达进一步说明皮肤在牦牛适应低氧环境中发挥作用。     

The influence of progesterone and prostaglandin F2α on decorin and the adhesion of caruncular epithelial cells of bovine placenta at early-mid pregnancy—Part II

One of the most important processes determining the proper course of gestation and its physiological termination in cows is the adhesion of epithelial cells allowing for direct contact of maternal and foetal parts of the placenta. Throughout pregnancy, placental cells are under strict hormonal control, which among others regulates the concentration and activity of specific proteins participating in the extracellular matrix remodelling of foetal membranes. The aim of the study was to evaluate the influence of progesterone and prostaglandin F_2α on the adhesion of epithelial cells at early-mid pregnancy in cows. Additionally, the impact of selected hormones on anti-adhesive properties of decorin was evaluated. Caruncular epithelial cells were isolated from healthy cows during pregnancy, immediately after slaughter. Primary cell cultures derived from the 2nd and 4th month of gestation were used in the experiments. The viability of cells was assessed by MTT assay. The adhesion of cells to fibronectin was measured spectrophotometrically. The activity of metalloproteinases was confirmed by the metalloproteinase assay. Progesterone (10^–5 and 10^–7 mol/L) and prostaglandin F_2α (10^–4_, 10^–5 and 10^–7 mol/L) increased the viability of bovine caruncular epithelial cells in the 2nd month of pregnancy. The treatment with prostaglandin F_2α significantly reduced the number of adherent cells from the 2nd month of gestation at the doses of 10^–4 and 10^–5 mol/L. Both progesterone and prostaglandin F_2α were shown to have an effect of decorin resulting in both a decrease in metalloproteinase activity and an increase in adhesion of cells to fibronectin.     

Effect of dietary Schizochytrium microalga oil on selected markers of low‐grade inflammation in rats

The objective of the present study was to evaluate a potential of Schizochytrium microalga oil to alleviate possible negative effects of high‐fat‐high‐energy diets. Forty adult male rats (Wistar Albino) were fed 7 weeks the diet containing beef tallow + evaporated sweetened milk (diet T) intended to cause mild obesity and low‐grade systemic inflammation. Consequently, the animals were divided into four groups by 10 animals each and fed either the T‐diet (control) or the diet containing 6% of safflower oil (S), 6% of fish oil (F) and 6% of Schizochytrium microalga oil (A), respectively, for another 7 weeks. The A‐diet decreased (p < 0.05) live weight to 86% and glycaemia to 85% of control, respectively; an effect of the S‐ and F‐diet on these markers was insignificant (p > 0.05). In comparison with control, higher (p < 0.05) deposition of eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) in the epididymal adipose tissue (EAT) of the A‐rats correlated with increased (p < 0.05) plasma adiponectin concentration, but it was without the effect (p > 0.05) on cellular adiponectin content in the EAT. Higher (p < 0.05) EPA+DHA deposition in the liver of the A‐rats correlated with higher expression (149% of control; p < 0.05) of the gene coding for peroxisome proliferator‐activated receptor gamma, and with lower expression (82% and 66%; p < 0.05) of the genes coding for adiponectin receptors AdipoR1 and AdipoR2; no relationship to the expression of receptor GPR120 was found. The A‐diet did not affect amount of the nuclear fraction of the nuclear factor kappa B in the liver, but increased plasma level of anti‐inflammatory cytokine TGF‐β1 (p < 0.05). The presented data agree with results of other in vivo rodent and human studies, but not with literature data regarding in vitro experiments: it can be concluded that the effects of dietary oils on inflammatory markers need further investigation.     

紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应

采用紫外诱变(波长260nm，功率15w)对一株产α-乙酰乳酸脱羧酶的枯草芽孢杆菌（Bacillus subtilis）BS059菌株进行诱变处理。结果表明，在紫外辐照时间为50s时，诱变效果较好。从正突变菌株中反复筛选，得到两株产酶量较高的菌株BS059-15和BS059-22，比原出发菌株的酶活分别提高了107.62%和162.25%。经过连续传代试验，证明其遗传性状稳定，为可遗传变异。