两种放线菌基因组DNA PCR模板制备方法的比较

[目的]比较制备放线菌基因组DNA PCR模板的两种方法液氮研磨法及TE煮沸法.[方法]以从河西走廊盐碱土壤中分离鉴定的8属16株菌为材料,包括糖丝菌属（Saccharothrix）Ⅳ23-3-4、类诺卡氏菌属（Nocardioides）Ⅴ22-5-1、原小单孢菌属（Promicromonospora）ⅨX5-4-3、小单孢菌属（Micromonospora）Ⅱ23-4-1、放线多孢菌属（Actinopolyspora）Ⅳ8-2-5、拟诺卡氏菌属（Nocardiopsis）Ⅱ8-4-3、链单孢菌属（Streptomonospora）DA01305、链霉菌属金色类群DA03401、链霉菌属金色类群DA01408、链霉菌属蓝色类群DA09140、链霉菌属金色类群DA01308、链霉菌属灰红紫类群DA05213、链霉菌属蓝色类群DA11417、链霉菌属粉红孢类群DA04401、链霉菌属灰红紫类群DA04402、链霉菌属球孢类群DA04307.对两种方法制备的PCR模板进行16S rDNA的扩增,PCR产物进行电泳检测.[结果]两种方法均能得到比较清晰的目的条带,但TE煮沸法简化了放线菌基因组DNA PCR模板制备的过程,可用于高通量放线菌PCR模板的快速制备.[结论]该研究为大批量菌株的快速鉴别和系统分类提供了有效的方法.     

Contribution of flavonoids to the antioxidant properties of common and tartary buckwheat

The objective of this study was to characterize the flavonoid compounds found in the different grain parts of common and tartary buckwheat, and to determine the contribution of these flavonoids to the antioxidant properties of buckwheat. Eight flavonoid compounds were quantified and their antioxidant activity determined by FRAP, DPPH, and ABTS assays. Of the flavonoid compounds identified rutin was the most abundant, particularly in tartary buckwheat, in which it comprised approximately 90% of total flavonoid content. Flavan-3-ols were detected in common but not tartary buckwheat, and quercetin was detected only in tartary buckwheat. Flavonoid content—in particular, levels of rutin, orientin, and/or epicatechin gallate—was found to influence the total antioxidant activity of buckwheat. Results from this study indicate that antioxidant activity is not only closely associated with flavonoid content, but that different flavonoids contribute differently to the total antioxidant activity of common and tartary buckwheat.     

Modeling the effects of microfluidization conditions on properties of corn bran

Corn bran was microfluidized through a 200-μm channel in the pressure range of 124.1–158.7 MPa for 1–5 passes following the central composite experimental design. Physicochemical properties and antioxidant properties of microfluidized bran samples were measured and fitted to the second order polynomial model. The response surface equations obtained showed that all the properties examined had a positive linear relationship with pressure and a negative quadratic relationship with number of passes except for ABTS radical scavenging activity which was quadratically related to both processing parameters. The number of passes generally had a more pronounced effect on the examined properties compared with pressure. Within the experimental range, the maximum values of swelling capacity, water-holding capacity, and oil-holding capacity were respectively 10.62 ml/g d.w. (at 158.7 MPa), 5.49 g water/g d.w. (at 158.7 MPa), and 4.61 g oil/g d.w. (at 124.1 MPa); the maximum values of surface reactive phenolic content, DPPH and ABTS radical scavenging activities were 148.80 mg/FAE g d.w. (at 158.7 MPa), 50.02 μmol TE/g d.w. (at 158.7 MPa), and 47.90 μmol TE/g d.w. (at 145.9 MPa), respectively. All maximum values of the properties occurred at 5 passes.     

Laboratory and Field Assessment of the Decagon 5TE and GS3 Sensors for Estimating Soil Water Content in Saline-Alkali Reclaimed Soils

The objective of this study was to assess, under laboratory and field conditions, the performance of the Decagon 5TE and GS3 soil water sensors in estimating the soil water content of saline-alkali reclaimed land in South Korea. The error due to increased bulk soil EC was greater for the 5TE sensor than for the GS3 sensor and was greater in soil with higher bulk soil EC. Therefore, it is recommended that the soil electrical conductivity be considered in the calibration process for the 5TE. The calibration equations developed for the 5TE and GS3 sensors during laboratory experiments were highly accurate. Testing of the 5TE and GS3 sensors showed very good agreement between actual VWC and VWC calculations resulting from the field-derived calibration. These results suggest that either sensor can be used to acquire accurate soil water content data in the field.     

Characterization of yield,sensitivity to Botrytis cinerea and antioxidant content of several rose species suitable for edible flowers

Yield, susceptibility to Botrytis and levels of antioxidants are important traits of fresh edible flowers. These parameters were determined for 12 garden rose cultivars selected as edible flowers: ‘Mount Shasta’, ‘San Francisco’, ‘Brandy’, ‘Maxim’, ‘Sweet Surrender’, ‘Amadeus’, ‘Eterna’, ‘Trier 2000’, ‘English sachet’, ‘Golden Celebration’, ‘Pat Austin’ and ‘Katharina Zeimet’. Yield of most cultivars ranged between 80 and 300 flowers/plant (for 64 weeks), which is higher than that produced by cultivars suitable for cut flowers. The yield makes the selected garden cultivars suitable for commercial growth as edible flowers.     

含盐砂土中5TE传感器应用的影响因素探究

5TE传感器由于其可以同时测量土壤温度、体积含水率和体积电导率的优势,越来越多地被应用于土壤评估中.为了更好地将5TE应用到盐渍化及低温等区域,通过室内砂柱正交实验探究含水率、盐分、温度这3个因素对5TE体积介电常数和体积电导率的影响.实验结果表明:①5TE体积介电常数除受含水率影响外,温度和盐分也会对其产生影响.饱和...     

小波动态PCA在过程监控中的应用

针对动态工业过程数据通常包含噪声的特点,提出了一种将小波分析与动态PCA相结合的WDPCA过程监控方法,即利用小波分析对过程原始数据进行消噪处理,然后通过动态PCA和T、Q图对过程监控。将WDPCA方法应用于TE过程,结果表明WDPCA方法能清晰的检测故障的发生,并比动态PCA有较高的准确率。