全文获取类型
收费全文 | 182篇 |
免费 | 9篇 |
国内免费 | 15篇 |
专业分类
林业 | 6篇 |
农学 | 9篇 |
3篇 | |
综合类 | 56篇 |
农作物 | 23篇 |
水产渔业 | 2篇 |
畜牧兽医 | 87篇 |
园艺 | 11篇 |
植物保护 | 9篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 8篇 |
2012年 | 11篇 |
2011年 | 9篇 |
2010年 | 5篇 |
2009年 | 13篇 |
2008年 | 15篇 |
2007年 | 15篇 |
2006年 | 16篇 |
2005年 | 9篇 |
2004年 | 7篇 |
2003年 | 2篇 |
2002年 | 7篇 |
2001年 | 7篇 |
2000年 | 10篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有206条查询结果,搜索用时 15 毫秒
1.
This report describes the use of 99m technetium pertechnetate (99m TcO4 − ) and 131 [for imaging of a metastatic thyroid carcinoma in a dog. The 131 ] imaging showed metastatic lesions undetected by the 99m TcO4 − imaging on 2 separate occasions. The possible mechanisms for the discrepancies between 131 I and 99m TcO4 − imaging of thyroid carcinomas are discussed. The use of 131 I for the imaging of functional thyroid carcinomas in the dog is recommended. 相似文献
2.
Phillip F. Steyn BVSc MRCVS MS Gregory Ogilvie DVM 《Veterinary radiology & ultrasound》1995,36(2):160-163
Technetium-99m methoxy-isobutyl-isonitrile (sestamibi) is a radiophartnaceutical used for the evaluation of myocardial perfusion. Increased uptake of sestamibi has also been documented in tumors. The objective of this study was to document the extracardiac biodistribution of 99m Tc sestamibi in the normal dog. Nine normal beagles were given 0.35 mCi/kg 99m Tc sestamibi intravenously, and 60 second images were made of the entire body at 1 hour post injection. A defined distribution pattern was recognized, with good visualization of the heart, liver, gallbladder, small intestine, kidneys, urinary bladder, popliteal lymph nodes, parotid salivary glands and zygomatic salivary glands. Splenic uptake was not seen.
Target to background ratios were calculated for all the regions listed, using background regions-of-interest with the smallest coefficient of variance for the denominator. The mean, range and standard deviation of these ratios are given. 相似文献
Target to background ratios were calculated for all the regions listed, using background regions-of-interest with the smallest coefficient of variance for the denominator. The mean, range and standard deviation of these ratios are given. 相似文献
3.
Federica Morandi DVM MS Gregory B. Daniel DVM MS Rebecca E. Gompf DVM MS Anne Bahr DVM MS 《Veterinary radiology & ultrasound》2004,45(2):97-102
Diagnosing right-to-left congenital cardiac shunts can be difficult. Cardiac catheterization and angiocardiography represent the traditional gold standard for diagnosis, but they are invasive. Nuclear scintigraphy using 99mTc-macroaggregated albumin (MAA) has been employed in humans as an alternate method of diagnosis. This study reviews eight dogs presented for evaluation of a suspect right-to-left cardiac shunt that were examined using 99mTc-MAA. In all, 2-4 mCi (74-148 MBq) of reduced particle 99mTc-MAA were injected IV in a cephalic vein and static images of the whole body, including right and left lateral, dorsal, and ventral views, were acquired for 60 s and stored into a 256 x 256 x 16 matrix. Shunt fractions were calculated. One dog with radiopharmaceutical distribution limited to the lungs did not have a shunt. Seven dogs had distribution of the radiopharmaceutical outside the pulmonary capillary bed, indicating bypassing of the pulmonary capillary circulation due to a right-to-left shunt. Four dogs had 99mTc-MAA within the brain. Three dogs that did not have brain uptake, but instead had a sharp cutoff of radioactivity at the level of the front limbs and neck, were diagnosed with reverse patent ductus arteriosus (PDA). The asymmetric distribution of the radiopharmaceutical is due to the location of the shunt, distal to the brachiocephalic trunk and left subclavian artery. Shunt fractions of dogs with extrapulmonary radioactivity ranged from 40% to 59%. Nuclear scintigraphy with 99mTc-MAA is a quick alternative method of diagnosing right-to-left cardiac shunts that permits quantification of shunt fraction. Distinguishing between reverse PDA and other right-to-left shunts may be possible based on the radiopharmaceutical distribution. 相似文献
4.
5.
6.
7.
对豇豆99-2,99-3,99-4三个优良株系性状进行比较试验.结果表明,豇豆99-2具有生长势好、早熟、高产、优质、商品性好等特点,丰产性尤为突出;前期产量12 304.5 kg/hm2,占总产量33 949.5 kg/hm2的36.68%,鲜荚比99-3、99-4早2~3 d上市.可作为早熟品种推广栽培. 相似文献
8.
一种非EDSV引起的鸭传染性减蛋症 总被引:5,自引:1,他引:5
自1999年5月以来,浙江省许多产蛋鸭发生了一种以产蛋率锐减为特征的新疾病,其中某地区67户养鸭专业户,共饲养蛋鸭15.7万羽,陆续进入产蛋高峰期后,有2375 羽鸭群的产蛋率突然下降,由原来的91.2%减到52.7%,同时伴有畸形蛋、沙壳蛋,蛋质低劣,蛋清稀薄,除少部分病鸭伴有烦躁不安和吵棚外,鸭群的精神体态基本正常,采食量略有下降,一般不引起死亡。起初养鸭户都误认为是当地饲料厂供应的饲料所致,后来发现该病具有传染性,疫病主要沿河流自上而下蔓延。在此后1个多月时间内,共有49户养鸭户12.3 万羽蛋鸭相继发病,产蛋率下降幅度为20%~80%,使用各种抗菌药物治疗无效,提高饲料营养也不起作用。该病发病急,传播迅速,通常在2~3天内产蛋率由80%~90%以上下降到30% ~40%,有的甚至更低,产蛋率越高,感染后下降幅度往往越大。病鸭从产蛋下降到康复需3 -7周时间,恢复后不能达到标准产蛋曲线,产蛋周期缩短。剖检病鸭主要表现为生殖器官病变,卵巢卵泡减少、萎缩,输卵管蛋白分泌部缩小,蛋白分泌腺减少,子宫萎缩,有的严重病例可见卵黄性腹膜炎,肝、心、肾和肺有斑点出血。该病的临床症状类似于减蛋综合征( EDS)[1],但是,用减蛋综合征灭活苗免疫预防不能有效保护。作者对病鸭体作无菌检查并分离了病毒(命名为YH99株),用0.2 μm滤器过滤后,经人工感染易感蛋鸭能成功诱导典型发病,再从病鸭中回收到同样病毒。将YH99株与鸡源减蛋综合征病毒(EDSV-AV127)[2~4]和鸭源减蛋综合征病毒[3](EDSV-JE 1,由上海市畜牧兽医站周锦萍提供)进行比较,结果发现: 相似文献
9.
[目的]获得有效的水牛RNA聚合酶Ⅲ启动子序列,为开展水牛源细胞的基因特异沉默研究奠定基础.[方法]通过启动子上、下游保守序列对水牛源启动子7SK、U6进行克隆和启动子关键顺式作用元件识别,利用一段针对EGFP的shRNA片段(shEGFP)对水牛7SK、U6启动子进行功能性分析,然后分别在水牛源及鼠源细胞中与pEGFP-N1共转染,转染48h后用荧光显微镜检测EGFP的表达情况,并用流式细胞仪和荧光实时定量PCR检测EGFP沉默表达情况.[结果]克隆获得水牛7SK、U6启动子序列分别为430和357 bp,其OCT-1(或CACCC盒)及TATA盒高度保守.连接shEGFP后与pEGFP-N1共转染细胞,通过荧光显微镜可观察到细胞发生了明显的荧光表达沉默现象;通过流式细胞分析,发现水牛7SK和U6启动子引导的shEGFP在水牛源细胞中沉默效率高达93.82%和87.45%;荧光实时定量PCR检测结果显示,在转染bu7SK-shEGFP和buU6-shEGFP的水牛源BFF细胞中,EGFP表达水平均显著低于其他物种启动子的细胞转染组(P<0.05),而在鼠源PT67细胞中,水牛启动子的启动效率与其他物种启动子差异不显著(P>0.05).[结论]水牛7SK和U6启动子可高效启动shRNA表达,且具有一定的物种特异性. 相似文献
10.
Laurie L. Head Gregory B. Daniel Timothy J. Becker David A. Lidbetter 《Veterinary radiology & ultrasound》2005,46(3):263-266
The normal feline pancreas has been evaluated using radiolabeled leukocytes (99mTc-HMPAO) and computed tomography. The purpose of this report is to describe a clinical case where both modalities were utilized to assess the inflamed feline pancreas. A nine year old female cat presented with anorexia, depression and some vomiting. Blood values were unremarkable. Radiographs and ultrasound were suggestive of pancreatitis. The cat's leukocytes were separated and labeled according to an established protocol. Whole body images were acquired immediately, at 5 and 30 min, and at 1, 2, 4, and 17 hours post injection. Approximately 48 h later, the animal was anesthetized and computed tomography of the abdomen was preformed both pre and post contrast. Surgical biopsies were taken. The distribution of the WBCs was similar to that documented in normal animals, however, at 2 h there was faint uptake seen in the region of the pancreas. This uptake became more intense at 4 h and persisted at 17 h. Computed tomography showed irregular margination of the pancreas, it was larger than normal and inhomogeneous. Contrast enhancement was inhomogeneous and its peak enhancement was not reached until 10 min post injection; normal feline pancreas enhances homogeneously and peaks immediately. Histopathology confirmed pancreatitis with lymphocytic, plasmacytic, neutrophilic and eosinophilic inflammation and fibrosis. Radiolabeled leukocytes can be used to document pancreatic inflammation and this is best seen 4 h after injection. Computed tomography allows superior visualization of the pancreas. Both the appearance and contrast enhancement pattern of the inflamed pancreas differ from normal. 相似文献