柔性坐标测量机参数辨识方法

设计了一种六自由度关节式柔性坐标测量机,并对该测量机测量方程中的参数进行了辨识,提出了一种快速简易的单点锥窝标定方法。该方法基于单点锥窝提供的空间单点基准,利用非线性最小二乘中的高斯-牛顿法,求解测量机测量方程的参数值。实验结果表明,该方法能够有效提高测量机的整体精度,且操作简单、计算效率高。     

以鄠邑区为例的基数转换要点研究

随着各级国土空间总体规划工作的推进,明确各地现状数据的工作日渐紧迫。以三调为基础的现状数据不能满足和符合国土空间总体规划的工作要求,所以在三调基础上科学准确的衔接规划编制所需的空间关联信息和现状数据,形成科学、准确的现状底图是保证空间规划编制科学有效的基础。因三调数据与其他关联数据在坐标、分类等方面存在差异,因此需要对其进行分类转换。本文在梳理三调工作分类和规划用途分类对应关系的基础上,以鄠邑区为例,对具体实践中遇到的主要问题提出相关建议。     

淮安市农业科技成果转化评价体系构建及实证分析

农业科技成果转化在我国农业现代化发展进程中发挥着至关重要的作用。本文在构建农业科技成果转化综合评价体系的基础上,运用层次分析法、专家征询法对淮安市农业科技成果转化进行实证研究,并在人才团队、企业创新、校企合作等方面提出加快地方农业科技成果转化的对策,以期充分发挥科技对农业经济社会发展的重要支撑作用。     

PEG介导的玉米弯孢叶斑病菌遗传转化

为了探索不同酶系组成对玉米弯孢叶斑病菌原生质体制备的影响,建立该病菌原生质体遗传转化系统,采用酶系混合物裂解菌丝体制备原生质体,采用PEG介导方法进行原生质遗传转化,通过PCR和Southern blotting技术对转化子进行验证。结果表明:1%溶壁酶+1%蜗牛酶+1%纤维素酶混合酶系为玉米弯孢叶斑病菌原生质体制备的最佳酶系,可以产生原生质体6.78×106 cfu/mL。用PEG介导方法转化共获得16个稳定的转化子,从中随机挑取5个转化子发现质粒pV2已被成功整合到基因组中。本研究获得了制备玉米弯孢叶斑病菌原生质体的最佳酶系,建立了PEG介导的原生质体遗传转化体系,为开展该菌致病相关基因克隆和基因功能研究提供了一种手段。     

果树中基因枪法遗传转化的研究进展

目前,应用于果树遗传转化的方法主要有农杆菌介导法和DNA直接导入法。介绍了DNA直接导入法中基因枪转化技术的原理、类型和基因枪的轰击参数、受体材料、基因型、轰击前后的培养条件等对其遗传转化频率的影响,并系统地概述了基因枪法在选择标记基因、报告基因、改良果树性状相关基因、启动子及多基因遗传转化中的应用研究进展,同时对果树基因枪转化研究中存在的问题及应用前景进行了讨论。     

Metabolie Engineering of Flower Color in Ornamental Plants

Abstract

Advances in the knowledge of plant pigment pathways at genetic, biochemical and molecular levels, and the establishment of genetic transformation methods for an increasing number of plant species have paved the way to genetic engineering of flower and plant color for ornamental purposes. From trial-and-error approaches based on the available few genes, the trend is now to comprehensively study the target species, genotypes and pigment pathways to precisely address specific breeding goals. This review examines the state of the art in this field, from the pioneering stages to fundamental and applied research on flavonoids, which is being extended to carotenoids and is also likely to involve other pigments in the near future.     

园林景观中的植物色彩设计——以青岛石老人生态旅游区植被改造为例

通过对青岛石老人生态旅游区的植被改造规划的分析,探讨植物不同器官和不同季节的色彩变化在园林景观规划设计中的意义。从生态旅游区的整体色彩效果出发,考虑背景色彩,着重应用原有的绿色基调,调配点缀不同的植物及色彩变化,营造丰富多彩和富于变化的植物色彩景观。     

渭河下游水沙变化趋势分析

本文通过对近6年渭河下游水沙量数据的统计和分析得出,渭河下游河段年内多沙期较河流主汛期有所提前;年径流量和年输沙量的变化趋势基本一致,而且自60年代至2005年华县站的水沙量持续呈递减趋势;河流的冲淤情况与该河段的降雨量有着直接的关系,当年平均降雨量小的时候河流以淤积为主,当年平均降雨量大的时候河流以冲蚀为主。针对这些特征,分析了渭河下游目前存在的主要问题。     

Land-cover and structural changes in a western Norwegian cultural landscape since 1865, based on an old cadastral map and a field survey

Many studies of land-cover and structural changes in cultural landscapes have used historical maps as a source for information about past land-cover. All transformations of historical maps onto modern coordinate systems are however burdened with difficulties when it comes to accuracy. We show that a detailed land survey of the present landscape may enable transformation of an old cadastral map directly onto the present terrain with very high accuracy. The detailed resulting map enabled us to locate remnants of semi-natural grasslands and man-made structures with continuity from 1865 and to test hypotheses about relationships between landscape changes and landscape characteristics. The main land-cover change 1865–2002 was decrease of arable fields, and addition of three new land-cover classes: horticultural, orchard and abandoned areas. Of the 330 man-made structures present in 1865, only 58 remained in 2002, while 63 new structures had been built after 1865. We found that semi-natural grasslands with continuity since 1865 were situated on ground with significantly lower production capacity than mean 1865 production capacity. The man-made structures with continuity since 1865 were also associated with areas with significantly lower production capacity than the 1865 mean, situated in significantly steeper terrain but not further from the hamlet. Our study illustrates the potential of digitised and accurately transformed historical cadastral maps combined with detailed field surveys for analysis of land-cover and structural changes in the cultural landscape.     

谷子转化及再生体系研究进展

谷子（Setaria italica）具有抗逆性强、适应性广、营养丰富等特点,是重要的区域性粮食作物之一。国内外谷子组织培养和遗传转化报道相对较少。对近年来谷子遗传转化方法和原理、组织培养再生体系的研究进展以及影响转化因素进行了总结,并对今后农杆菌介导谷子转化应注意的问题和前景作了简单讨论。