倾斜圆盘勺式精密排种器投种过程分析

以种子几何形状和尺寸的变化为基础，分析了倾斜圆盘勺式精密排种器的结构参数和工作参数对实际投种角的影响以及相邻两粒种子在投种过程中的时间间隔偏差，进而建立了种子在种床内粒距实际分布的数学模型。分析结果表明，种子的几何尺寸、投种轮的高度、播种机的前进速度和投种轮的直径影响投种时种子运动的速度和方向，从而导致实际粒距的变化。为保证排种器顺利投种，建立了投种口长度与排种器参数间关系和参数组合的限制条件。     

基于儿童体验需求的温泉泡池设计研究

现在家庭,存在以孩童为中心的结构特点。本文以0～12岁的儿童为研究对象,通过儿童心理特征构成分析,进而从感官、行为、情感、思考、关联等方面来研究儿童对温泉泡浴的体验特征,并总结出儿童温泉需求的特点。基于研究结果,从温泉布局特点、流线空间关系、泡池形态规格、泡池材质水温、以及周边小环境等方面提出相应的设计思考,为温泉旅游度假区等相关项目中有关儿童泡池区域设计方面的内容提供一定的技术参考。     

Clinical evaluation of the anaesthetic sparing effect of brachial plexus block in cats

ObjectiveTo evaluate the isoflurane sparing effect and the post-surgical analgesia provided by a brachial plexus block (BPB) in cats undergoing distal thoracic limb surgery.Study designProspective randomized blinded clinical study.AnimalsTwenty client-owned cats.MethodsCats were assigned to receive either no BPB (group NB) or a nerve stimulator guided BPB (group BPB) using lidocaine (3.6 mg kg^?1) and bupivacaine (1.2 mg kg^?1). Pre-medication consisted of midazolam and ketamine intravenously (IV). Anaesthesia was induced with propofol IV to effect and maintained with isoflurane delivered in oxygen and a continuous rate infusion of fentanyl (2 μg kg^?1 hour^?1). End-tidal isoflurane concentration (Fe′ISO) was adjusted every 3 minutes guided by changes in cardiorespiratory parameters and reflexes present, to maintain a stable depth of anaesthesia. Five time points were chosen to record all parameters and compare values between groups. Recovery and post-operative pain assessment were performed using a visual analogue scale (VAS) at 15 and 45 minutes after extubation and thereafter at hourly intervals until 5 hours after placement of the BPB.ResultsNo clinically significant differences were seen for heart rate, respiratory rate and non-invasive blood pressure between groups. Mean Fe′ISO was significantly lower in group BPB compared with group NB at all time points. In group NB, all intraoperative measurements of Fe′ISO were significantly higher compared with baseline (3 minutes before start of surgery) measurements. During recovery, VAS scores for group BPB were significantly lower than for group NB. Additional analgesics were needed in all cats within the study period.Conclusion and Clinical relevanceIn cats undergoing orthopaedic surgery of the thoracic limb, BPB reduced intra-operative isoflurane requirement and pain during the early post-operative period when compared with procedures without a BPB. BPB is a useful adjunct to anaesthesia in such cases.     

我国南方香草植物栽培技术及典型应用研究

介绍香草植物及其分布、用途,阐述影响香草植物生长的因素、南方栽培技术标准和栽培关键技术要点,并论述香草植物在温泉旅游业中的典型应用.     

Evidence of fish and human pathogens associated with doctor fish (Garra rufa,Heckel, 1843) used for cosmetic treatment

Doctor fish (Garra rufa, Heckel, 1843) are increasingly used for cosmetic treatment raising particular concerns regarding the potential transmission of infections to clients. Investigations of microbial causes undertaken in two outbreaks of mortality among G. rufa used for cosmetic treatment revealed the presence of multiple bacteria, including both fish and human pathogens such as Aeromonas veronii, A. hydrophila, Vibrio cholerae, Shewanella putrefaciens, Mycobacterium marinum and M. goodii. This range of bacteria indicates an intense microbial proliferation involving multiple pathogens, most likely induced by the poor health condition of the fish. Most of the detected pathogens are well‐known agents of zoonosis. Indeed, M. goodii is an emerging nosocomial human pathogen that has never been detected in fish to date, nor in other animals. This first detection of M. goodii associated with fish infection points out a new zoonotic potential for this pathogen. These findings point out that handling, poor environmental conditions and the presence of fish pathogens, that can compromise the immune system of fish, can result in a mixed microbial proliferation and increase the spread of waterborne bacteria, including zoonosis agents. Accordingly, the microbiological surveillance of fish used for cosmetic treatment is extremely important, particularly in association with mortality outbreaks.     

牛源MRSA新疆流行株的分离鉴定及spa基因多态性分型

[目的]明确新疆地区牛源耐甲氧西林金黄色葡萄球菌(MRSA)流行株的流行特点,为有效防控新疆奶牛乳房炎和子宫内膜炎提供参考依据.[方法]采用分离培养、生化鉴定、药敏试验等方法对牛源MRSA进行鉴定,并以PCR扩增牛源MRSA新疆流行株spa基因的X区,测序结果列提交至spa分型数据库(http://www.ridom.de/spaserver/)进行spa基因多态性分型.[结果]从221份样品中共检测出71株金黄色葡萄球菌,其中对苯唑西林抑菌直径小于10 mm的有8株;而以mecA引物进行PCR扩增,发现扩增结果呈阳性的有13株,MRSA检出率为18.3%.spa基因分型有4种,分别为t779、t2883、t13751和t1939,其中5株为t779(r08),4株为t2883(r07-r23-r21-r17-r34),2株为t13751(r07-r23-r12-r21-r17-r12),2株为t1939(r07-r23-r02-r34),即t779(r08)为MRSA新疆流行株的主要spa基因分型.[结论]MRSA在新疆不同地区均有分布,且其耐药情况不断变化,以t779(r08)为MRSA新疆流行株的主要spa基因分型.     

胶东地区不同家禽MSSA和MRSA的流行分布与耐药性分析

通过了解胶东地区肉鸡、蛋鸡和水禽(鸭、鹅)中甲氧西林敏感/耐药金黄色葡萄球菌(MSSA/MRSA)的流行情况及耐药现状,为临床合理用药、有效控制耐药菌株的流行传播提供依据。采用细菌常规分离培养、质谱/PCR鉴定、微量肉汤稀释法和spa分型等方法,对2021年8月至10月在青岛地区采集的2730份禽源咽拭子进行MSSA及MRSA分离鉴定、spa分型及药敏试验,利用SPSS26.0软件和BioNumericsv7.6软件进行数据处理分析。2730份禽源咽拭子中共获得金黄色葡萄球菌742株(27.18%),其中MSSA 528株(19.34%),MRSA 214株(7.84%)。三种家禽菌株分离率依次为水禽(38.33%)、蛋鸡(28.45%)、肉鸡(20.33%)。351株代表性金黄色葡萄球菌共获得16种spa型别,MSSA(70.80%)和MRSA(95.30%)均是以t899为主。部分型别仅在蛋鸡(t010、t002、t3155)或肉鸡(t571、t011)或水禽(t1793、t5268、t267)中分离到。MSSA和MRSA对青霉素类、喹诺酮类等多种药物显示出高水平耐药,分别对8种和...     

动物源金黄色葡萄球菌耐药性、oqxAB和fexA基因流行性及spa分型研究

为了解河南省不同动物源金黄色葡萄球菌的耐药情况、oqxAB和fexA基因的流行情况,分析spa基因多态性分型特点。用微量肉汤稀释法测定了分离菌对10种抗菌药物的敏感性,利用PCR方法检测金黄色葡萄球菌中的oqxAB和fexA耐药基因,扩增spa基因的X区域进行分型研究。结果表明,130株金黄色葡萄球菌分离株对大多数药物产生耐药,青霉素、红霉素、林可霉素、四环素、环丙沙星、恩诺沙星、氟苯尼考和喹乙醇对分离菌的耐药率分别为94.62%,95.38%,92.31%,70.77%,55.38%,57.69%,32.31%,19.23%,但对替加环素、万古霉素较敏感。PCR检测发现,35株携带fexA基因,检出率为26.92%。17株携带oqxAB基因,检出率为13.08%。此外,有10株同时携带oqxAB和fexA基因,耐药性检测发现这10株菌耐药性较为严重。130株分离菌中107株菌可通过spa分型,分为21种spa型,最常见的spa型是t15075 (15.38%),其次是t189 (13.85%),t034 (9.23%),t091 (7.69%),t127 (6.92%),t164 (6.15%)。此外,只有t034 (9.23%)和t3512 (3.85%)能够出现在3种不同动物源(分别是牛、猪、鸡及牛、猪、鸭)的菌株中。综上,oqxAB和fexA基因在河南省的动物养殖场中已有一定的流行,同时携带oqxAB和fexA基因的菌株耐药性较为严重,spa型别具有多样性和差异性的特点,故临床应加强耐药性及分型监测。