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1.
M. Sandholm A. Vidovic A. Puotunen-Reinert S. Sankari K. Nyholm H. Rita 《Acta veterinaria Scandinavica》1995,36(2):255
The discriminating ability of 15 parameters alone or in combinations, including results from analysis of plasma endotoxin, the Nycomed plasma D-Dimer test and phospholipase A2, were analyzed to predict morbidity and mortality in equine gastrointestinal colic. Endotoxaemia was a characteristic feature of the colic horses. The problem of adequately predicting non-survivors among colic horses required several parameters to be included in the logistic model: if the “classical parameters”, (heart rate, respiratory rate, PCV, anion gap) were included in the model, addition of plasma D-dimer, phospholipase A2, and Cl- significantly improved the predictive value of the logistic model. Increasing heart rate and D-dimer together with decreasing chloride was a risk factor for nonsurvival. The sensitivity of this three-parameter logistic model to predict nonsurvival was 78% and specificity 77%. The Nycomed D-Dimer test is recommended as a horse-site test to predict disseminated intravascular coagulation and nonsurvival in equine colic. 相似文献
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山梨醇对李果肉组织总RNA提取的影响 总被引:2,自引:0,他引:2
探讨了不同浓度的山梨醇清洗溶液对李果肉组织中RNA提取效果的影响。经琼脂糖凝胶电泳、核酸蛋白分析仪和RT-PCR等方法对RNA质量和产率进行了分析,结果表明:李果肉组织研磨后用山梨醇清洗溶液处理有利于总RNA的提取,而不添加山梨醇的清洗溶液,提取液中检测不到RNA,当清洗溶液中山梨醇浓度为1.10mol/L时,李果肉组织总RNA的产率明显高于山梨醇浓度为0.35 mol/L和3.00 mol/L时的产率;以不同贮藏天数的李果实为试材提取总RNA,其结果是一致的;通过RT-PCR扩增蛋白延伸因子EF-2基因,结果证明本试验提取的总RNA可以用于基因转录表达特性分析。 相似文献
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目的:以芒果、木糖醇、山梨糖醇、低聚果糖为主要原料,研发一款品质优良且具有预防龋齿,促进消化吸收,提高免疫力等功能的芒果无糖软糖。方法:以感官评价为指标,通过单因素试验研究木糖醇、山梨糖醇、低聚果糖的添加比例、复合凝结剂添加量以及柠檬酸添加量等对芒果软糖感官品质的影响,通过正交试验,模糊数学法优化芒果软糖配方。结果:芒果软糖的最佳配方为芒果汁添加量50%,柠檬酸添加量0.2%,复合凝结剂添加量10%,木糖醇、山梨糖醇、低聚果糖的添加比例2:3:1(添加量为35%);二十二碳六烯酸添加量1%,维生素C添加量0.5%。结论:此配方下的芒果软糖不仅色泽均匀、弹性适中,而且形态饱满,酸甜可口,具有一定的保健功效。 相似文献
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Summary Central Asian apricot germplasm was used in hybridizations with California adapted apricots to increase Brix levels and improve fresh eating quality. Fruit from parental trees, the F1 hybrid and two backcross families were evaluated for fruit quality traits and analyzed by HPLC for specific sugar content. The F1 hybrid between Central Asian and California adapted apricots was intermediate to its parents in many of the evaluated characteristics and levels of specific sugars. When the F1 hybrid was backcrossed to California adapted apricots ‘Lorna’ and ‘Robada,’ the resulting hybrids were diverse in Brix, juice acidity, fruit size and profiles of specific sugars. Glucose: fructose ratios higher that 3.3 were encountered in fruit from five of the 22 analyzed seedlings, and fructose: sorbitol ratio ranged from 0.67 to 6.46. Brix and total sugar content correlated significantly with each other and with both sucrose and glucose. No significant correlations existed between sorbitol and any of the other analyzed sugars, nor with Brix or total sugars. The results demonstrated the extent of sugar profile modification possible in California adapted apricots after just two generations of breeding with Central Asian apricot germplasm. 相似文献
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以青种枇杷为试材,在果实成熟前50d左右分别喷施100、200、300mg·L-1吲熟酯及0.1%钼酸铵和200mg·L-1吲熟酯+0.1%钼酸铵,研究吲熟酯和钼酸铵对果实生长发育过程中糖积累以及蔗糖与山梨醇代谢相关酶活性变化的影响。结果表明:200mg·L-1吲熟酯处理对增加果实的果糖和葡萄糖积累最为明显。所有处理的果实山梨醇含量均随果实发育呈先下降后上升的趋势。100mg·L-1吲熟酯处理的蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性在5月21日后呈明显的下降趋势,其他处理的蔗糖代谢相关酶活性均呈上升趋势;山梨醇代谢相关酶活性总体上呈下降趋势。综上所述,200mg·L-1吲熟酯处理对青种枇杷果实糖积累有较显著的促进作用。 相似文献
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《Communications in Soil Science and Plant Analysis》2012,43(7-8):1245-1259
Abstract Hot water extraction (HW) is time‐consuming, highly variable, and losing popularity as the standard method for estimating plant‐available boron (B) in soil. Proposed alternatives are not widely used and guesstimation is replacing assessment at many soil test facilities. Mehlich 3 is increasingly promoted as a universal extractant, and diethylenetriaminepentaacetic acid (DTPA)–sorbitol and pressurized hot water (PHW) are effective and comparable to hot water extraction but also simpler and easier. Mehlich 3 B extraction has been compared mainly to hot water extraction. Because Mehlich 3 usage would be limited to neutral to acid soils, this study used a limed acid Darco loamy fine sand (loamy, siliceous, semiactive, thermic Grossarenic Paleudult) from eastern Texas to which 10 rates of B were applied followed by either incubation without plants or planting to alfalfa in greenhouse pots. Mehlich 3 extraction of soils obtained from a long‐term experiment on Darco soil from which alfalfa yield response has already been related to hot water, DTPA–sorbitol, and PHW is reported. The purpose was to determine the efficiency of Mehlich 3 B extraction compared to hot water, PHW, and DTPA–sorbitol in these B‐fertilized soils. Mehlich 3–extractable B significantly correlated with the rate of B application to incubation, greenhouse, and field soils and with B concentration and total B uptake in alfalfa in a greenhouse experiment. However, yield responses to B application were not observed in the greenhouse study. In the field where B response to B application was observed, Mehlich 3–extractable B did not correlate with alfalfa yield, whereas hot water and pressurized hot water did. In considering Mehlich 3 for B extraction, be aware that some older inductively coupled plasma (ICP) models may have significant drift when B is measured in Mehlich 3 extractant. In the current study, this problem was overcome with a new model instrument. Although effective in estimating B levels imposed on soils by fertilizer application, Mehlich 3 could not predict yield and thus cannot currently be recommended as a “universal” extractant to include B. 相似文献
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桃果实发育过程中山梨醇及酶活性变化 总被引:3,自引:1,他引:2
碳水化合物的种类和含量是决定果实品质的重要因素,果实中碳水化合物的转化、积累除受内源激素、韧皮部卸载等因子影响外,很大程度上依靠其代谢相关酶活性的调控.糖是植物生长、发育的一个重要的、多重的角色,是果实生长发育的基础物质,同时为果实细胞膨大提供渗透动力.山梨醇属于糖醇类,是山梨糖光合同化产物,可以作为可溶性贮存物质,也可作为类似于蔗糖的一种同化物运输物质.此外,山梨醇与提高植物的抗逆性、改善果实风味也有关(周睿等,1993;Beruter et al.,1997),其在果实内能通过其代谢酶被迅速转化(周睿等,1993;Bianco et al.,2002),山梨醇脱氢酶(sorbitoldehydrogenase,SDH)和山梨醇氧化酶(sorbitol oxidase,SOX)是参与山梨醇代谢的关键酶,其中SDH催化山梨醇转化成果糖与葡萄糖,SOX催化山梨醇氧化成葡萄糖(周睿等,1993;Yamaki et al.,1986). 相似文献