全文获取类型
收费全文 | 2512篇 |
免费 | 132篇 |
国内免费 | 254篇 |
专业分类
林业 | 100篇 |
农学 | 553篇 |
基础科学 | 8篇 |
112篇 | |
综合类 | 885篇 |
农作物 | 180篇 |
水产渔业 | 357篇 |
畜牧兽医 | 346篇 |
园艺 | 214篇 |
植物保护 | 143篇 |
出版年
2024年 | 3篇 |
2023年 | 23篇 |
2022年 | 29篇 |
2021年 | 58篇 |
2020年 | 58篇 |
2019年 | 80篇 |
2018年 | 47篇 |
2017年 | 65篇 |
2016年 | 92篇 |
2015年 | 74篇 |
2014年 | 96篇 |
2013年 | 126篇 |
2012年 | 154篇 |
2011年 | 176篇 |
2010年 | 146篇 |
2009年 | 118篇 |
2008年 | 167篇 |
2007年 | 149篇 |
2006年 | 159篇 |
2005年 | 103篇 |
2004年 | 102篇 |
2003年 | 103篇 |
2002年 | 84篇 |
2001年 | 60篇 |
2000年 | 55篇 |
1999年 | 63篇 |
1998年 | 59篇 |
1997年 | 60篇 |
1996年 | 51篇 |
1995年 | 44篇 |
1994年 | 43篇 |
1993年 | 39篇 |
1992年 | 37篇 |
1991年 | 33篇 |
1990年 | 25篇 |
1989年 | 20篇 |
1988年 | 18篇 |
1987年 | 12篇 |
1986年 | 10篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1982年 | 10篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1955年 | 6篇 |
排序方式: 共有2898条查询结果,搜索用时 15 毫秒
1.
同源四倍体水稻与狼尾草杂交结实的胚胎学证据 总被引:6,自引:0,他引:6
在以6份同源四倍体水稻为杂交母本与狼尾草进行属间杂交中发现,其结实率达到0.84%~2.06%,而在以二倍体水稻为母本的属间杂交中却没有获得结实种子.利用激光扫描共聚焦显微技术对属间杂交结籽的特殊生殖现象进行了观察研究,试图寻找其杂交结实的胚胎学证据.结果表明,狼尾草的花粉粒不能在二倍体水稻的柱头上萌发,说明二倍体水稻与狼尾草的生殖隔离很严格,两者很难杂交.然而,狼尾草的花粉粒在同源四倍体水稻的柱头上能萌发,花粉管能在花柱中伸长并能将雄配子送入胚囊内与雌配子融合成受精卵.同源四倍体水稻与狼尾草的受精作用比较特殊,包括精细胞与卵细胞的单受精作用、精细胞与次生极核的单受精作用和正常的双受精作用,总受精率和总成胚率分别为6.36%~11.68%和4.11%~9.44%.试验结果为解释同源四倍体水稻与狼尾草杂交结籽的特殊生殖现象提供了有力的胚胎学证据. 相似文献
2.
3.
Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs. 相似文献
4.
Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses.
The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content.
Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in 相似文献
5.
6.
7.
经过冬蜜花期对以湖北鄂西中蜂为母本、广东粤东地中蜂为父本的杂交一代与本地中蜂进行比较,结果表明:杂交一代的繁殖力和产蜜量分别比本地中蜂高4.4%和11%。夏季乌桕花期对回交代进行对比结果表明:其繁殖力和产蜜量分别比本地中蜂高6%和21%。 相似文献
8.
本文应用聚合酶链反应(PCR)技术从构建的新城疫病毒(NDV)cDNA文库中扩增含编码F糖蛋白前体──Fo酶切位点序列的359bp的F蛋白基因cDNA片段。将此359bpcDNA片段经光敏生物素标记后,即成NDV-cDNA探针。该探针能特异性地从感染的尿囊液中检测出NDV强毒株和疫苗毒株的基因组RNA,而不与IBDv-dsRNA、AIBv-ssRNA、EDS76-dsDNA、MDV-dsDNA,FPV-dsRNA及AILV-dsDNA发生交叉杂交反应。试验结果表明:尽管该探钎含有编码Fo蛋白酶切位点序列的碱基顺序,但它还是不能把NDV的强、弱毒株区分开。这说明NDV强、弱毒株比区域内的碱基存在着相当大的同源性。不过,此探针对NDV来说具有特异性,这就为NDV的诊断技术开创了基因水平检测的新途径。 相似文献
9.
10.
To determine the potential of sexual reproduction among host-adapted populations of Phytophthora infestans sensu lato in Ecuador, 13 A1 isolates belonging to clonal lineages US-1, EC-1 and EC-3, and 11 A2 isolates belonging to the clonal lineage EC-2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US-1, two EC-1 and one EC-3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC-1 lineage produced more oospores of healthy appearance than did matings with isolates of US-1 or EC-3. In the second experiment, the oospores of 35 crosses (21 EC-1 × EC-2; 10 US-1 × EC-2; four EC-3 × EC-2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty-three single-oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose-6-phosphate isomerase ( Gpi ) and peptidase ( Pep ) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the Pep locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of P. infestans sensu lato in Ecuador. 相似文献