共轭亚油酸(CLA)对动物营养效应研究进展

本文对共轭亚油酸对动物的免疫作用、抗癌及防止动脉粥样硬化作用、抗氧化作用等进行了综述 ,重点论述了共轭亚油酸的营养再分配作用 ,并对有待继续研究的问题进行了探讨。     

Acclimation abilities of three tropical rainforest seedlings to an increase in light intensity

Current practices in many nurseries involve the germination of tropical rainforest seedlings in shaded conditions and transferral, at a later date, to environments with greater light intensities. Determination of the ability of these seedlings to acclimate to increased light intensities will allow seedling stock to be processed with maximum efficiency within the nursery. The acclimation abilities of three species, Agyrodendron actinophyllum, Cardwellia sublimis, and Flindersia brayleyana, commonly found in north Queensland's rainforests were investigated in this study. These particular species are highly valued for their cabinet timber qualities and are being reared in nurseries for use in reforestation trials and programs in north Queensland. Seedlings were initially raised in greenhouse conditions under two layers of shade cloth (16% of full sunlight) and then transferred into full sunlight at three different ages (3 weeks, 10 weeks, 14 weeks). Upon transfer, organ ratios and the direction of dry matter distribution was determined for each species and age group. Approximately 3 months after the seedlings were transferred, the acclimation ability of each species and age group was then determined. Dry matter distribution was found to change with age, irrespective of light environment. Individuals within a species with larger root systems and thicker or more dense leaves had a greater acclimation ability than those with smaller root systems and thinner or less dense leaves. Furthermore, individuals within a species whose dry matter distribution upon transfer was directed towards developing a large root system, and a small photosynthetic area and mass, had a greater acclimation ability than those whose dry matter distribution was directed away from such morphologies. Awareness of these relationships allows a better understanding of seedling response to gap formation in natural forests, and also allows plant nursery operators to make a more informed decision about when to move seedlings to environments with a higher light intensity.     

永城县土壤养分变化动态与培肥措施

对永城县1982年至1987年土壤养分变化动态进行了定点分析研究.共分析了三十个乡的257个土样。土壤有机质和氮素含量1987年比1982年相应提高25%和41.4%;而速效磷和钾则相应降低36.1%和89.9%;微量元素有效锌、硼、钼含量均属中低级水平。据此提出了相应的培肥措施.     

A novel approach to studying the effects of temperature on soil biogeochemistry using a thermal gradient bar

The temperature dependence of chemical reaction rates and microbial metabolism mean that temperature is a key factor regulating soil trace gas emissions and hydrochemistry. Here we evaluated a novel approach for studying the thermal response of soils, by examining the effects of temperature on gas emissions and hydrochemistry in (a) peat and (b) soil from a Sitka spruce plantation. A thermal gradient was applied along an aluminium bar, allowing soil to be incubated contemporaneously from 2 to 18 °C. The approach demonstrated clear differences in the biogeochemical responses of the two soil types to warming. The peat showed no significant emission of CH₄ at temperatures below 6 °C, while above 6 °C, a marked increase in the rate of release was apparent up to 15 °C (Q₁₀ = 2.5) with emissions being similar between 15 and 18 °C. Conversely, CH₄ emissions from the forest soil did not respond to warming. Nitrate availability in the peat decreased by 90% between 2 and 18 °C (P < 0.01), whereas concentrations in the forest soil did not respond. Sulphate availability in the peat decreased significantly with warming (60%, P < 0.01), while the forest soil showed the opposite response (a 30% increase, P < 0.01). Conventionally, thermal responses are studied by incubating individual soil samples at different temperatures, involving lengthy preparation and facilities to incubate samples at different temperatures simultaneously. Data collected on a given thermal response is usually limited and thus interpolated or extrapolated. The thermal gradient method overcomes these problems, is simple and flexible, and can be adapted for a wide range of sample types (not confined to soil). Such apparatus may prove useful in the optimization of management practices to mitigate the effects of climate change, as thermal responses will differ depending on land use and soil type.     

Development of Immune Response to Experimental Bovine Trichophyton vervucosum Infection

Abstract— Cell mediated and humoral immune responses to experimental Trichophyton verrucosum infection were assessed by sequential cutaneous biopsies, antibody assessments and microscopic monitoring of fungal presence. Histopathologic examination showed the accrual of lymphocytes and other inflammatory cells in the dermis of infected sites. Immunoperoxidase staining of frozen sections with monoclonal antibody preparations revealed an influx of macrophages, BoCD4+ and B0CD8+ lymphocytes and γδ T cells from the 5th day to the 33rd day of infection. A moderate influx of B cells was observed. Protein G-colloidal gold staining revealed the presence of immunoglobulins in the dermis and superficial epidermal layers. Trichophyton specific serum antibodies appeared between days 33 and 55. Microscopic assessment of infected tissues revealed an increase in T, verrucosum elements (mycelium and ectothrix spores) from days 19 to 55. Fungal elements in infected areas did not decrease until after both humoral and cell mediated elements of the immune response were established. These responses imply a combination of cell mediated and humoral events were associated with T. verrucosum immunity and clearance in the calf. Résumé— Le réponse immunitaire humorale et cellulaire a une infection expérimentale àT. verrucosum a été appréciée par des biospsies cutanées successives, des dosages d'anticorps et la recherche microscopique de champignons. L'examen histopathologique a montré un afflux de lymphocytes et d'autres cellules inflammatoires dane le derme des sites infectés. Les marquages en immunopéroxydase par un anticorps monoclonal de coupes congelées a montré un influx de macrophages, lymphocytes BoCD4+ et BoCD8+ et des cellules T γδ, du 5e au 33e jour de l'infection. Un marquage par une protéine G—or colloidal a révélé d'immunogolglobulines dans le derme et les couches supéerficielles de l'épiderme. Les anticorps spécifiques de Trichophyton sont apparus entre 33 et 55 jours. L'examen microscopique des tissus infectés a révélé une augmentation du nombre d'éléments de T. verrucosum (mycelium et spores ectothrix) du 19e au 55e jours. Les éléments fongiques dans les zones infectées n'ont pas diminué avant que les réponses humorales et cellulaires ne solent établies. Ces résponses impliquent qu'une coopération des réponses humorales et cellulaires étaient associées dans l'immunité et la défense contre T. verrucosum. Zusammenfassung— Die zellvermittelten und humoralen Immunantworten auf die experimentelle Infektion mit T. verrucosum wurden durch eine Serie von Hautbiopsien, Antikörperuntersuchungen und mikroskopischer Untersuchung auf das Vorhandensein von Pilzen ausgewertet. Die histopathologische Untersuchung zeigte eine Ansammlung von Lymphozyten und anderen Entzündungszellen in der Dermis der infizierten Stellen. Die Immunperoxidasefärbung der Gefrierschnitte mit monoklonalen Antikörperzubereitungen zeigte einen Influx von Makrophagen, BoCd4-und BoCD8-Lymphozyten und gamma-delta-T-Zellen vom 5. bis zum 33. Tag der Infektion. Es wurde auch ein mäßiger Influx von B-Zellen beobachtet. Die Protein-G-kolloidale Goldfärbung zeigte die Anwesenheit von Immunglobulinen in der Dermis und den oberflächlichen epidermalen Schichten. Trichophyton-spezifische Serumantikörper traten zwischen Tag 33 und 55 auf. Die mikroskopische Untersuchung infizierter Gewebe zeigte eine Zunahme von T. verrucosum-Bestandteilen (Myzel und exktothrixe Sporen) vom Tag 19 bis 55. Pilzteile in infizierten Bereichen verminderten sich weder, nachdem humorale, noch nachdem zellvermittelte Elemente der Immunantwort auftraten. Diese Reaktionen deuten an, daß eine Kombination von zellvermittelten und humoralen Vorgängen im Zusammenhang mit T. verrucosum-Immumtät und Abheilung beim Kalb vorliegt. Resumen Por medio de biposias cutáneas secuenciales, medida de anticuerpos y exámen microscópico de presencia de hongos, se estudió la respuesta inmunitaria de tipo humoral y celular producida por la infección experimental con T. verrucosum. El exámen histopatológico reveló la presencia de agregación de linfocitos y otras células inflamatorias procedentes de la dermis de los puntos infectados. La tintura por medio de inmunoperoxidasa de las secciones congeladas, con preparaciones monoclonales de anticuerpos, demostró un aflujo de macrófagos BoCD4 + y BoCD8 + linfocitos y linfocitos, Tαδ, desde el quinto hasta el día 33 la infección. También se observó un aflugo moderado de linfocitos B. La tintura aúrica de proteina coloidal G reveló la presencia de inmunoglobulinas en al dermis y capas superficiales de la epidermis. Los anticuerpos específicos para la especie Trichophyton aparecieron entre los días 33 y 55. El exámen microscópico de los tejidos afectados demostró un incremento, de los elementos füngicos T. verrucosum (micelio y esporas exótricas) desde los días 19 al 55. Los elementos fúngicos en áreas infectadas no disminuyeron hasta después del establecimiento de ambos tipos de respuesta inmunitaria, humoral y celular. Estas respuestas implican que la combinación de ciertos fenómenos de inmunidad celular y humoral, están relacionados con la desaparición y la inmunidad de la infección producia por T. verrucosum en el ternero.     

Correlated Responses in Two Traits when Selection is Practised on their Ratio

In many fields of application in plant breeding and crop science, ratios of two component traits X and Y are of interest (harvest index in cereals, leaf-to-stem ratio in forage legumes, height-to-diameter ratio in forest trees etc.). When selection is practised on the ratio X/Y of two traits X and Y, the experimenter may be interested in the resulting changes of both trait means. Based on improved approximations for the covariances between X and X/Y and between Y and X/Y and for the variance of X/Y the changes in the means of X and Y can be predicted by applying the regression approach from conventional selection theory. Explicit expressions for these correlated responses in X and Y when selection is practised on their ratio X/Y are derived and discussed. The different outcomes (decrease, zero change or increase) for the selection pressures on X and Y are characterized by phenotypic coefficients of variation of X and Y, phenotypic and genotypic correlations between X and Y and heritabilities of X and Y.     

赖氨酸占蛋白质及饲粮的比例对杜洛克猪生长肥育性能及胴体品质的影响

杜洛克猪48头,体重27kg左右,分6组。试验前期(27～60kg)日粮的消化能均为13.67MJ)/kg,后期(60～90kg)均为13.44MJ/kg。粗蛋白1、2、3组前期均为18％,后期均为16％;4、5、6组前期均为16％,后期均为14％。同时配合3种赖蛋比水平6.6～6.9％,6.1～6.25％及5％。试验结果表明:4、5、6组平均日增重、饲料利用率及胴体瘦肉率都略高于1、2、3组,但差异不显著(P>0.05)。在前期,以第5组平均日增重最高(达782克),极显著高于其他各组(P<0.01);饲料利用率2.41,极显著好于其他各组(P<0.01)。后期以第5组平均日增重最高(875克)。试验前期,第5组获得最佳的日增重(822克),极显著高于其他各组(P<0.01),饲料利用率2.98,胴体瘦肉率65.79％,与其他各组差异不显著(P>0.05)。在两种粗蛋白水平组内,均以赖蛋比6.1～6.25％的第5组和第2组生长速度最快(822克和778克)。说明在相同的能量水平下,赖蛋比是影响生长速度的主要因素。     

乳酸菌与中药合生元对肉鸡生长性能及饲料利用率的影响

本试验分别向肉仔鸡日粮中添加等量的抗生素和乳酸菌与中药合生元制剂,通过测定肉仔鸡的各项生长指标和饲料利用率指标,探讨乳酸菌与中药合生元在肉鸡生长和饲料利用率方面替代抗生素的优势.结果表明,试验组与对照组在生长性能和饲料利用率上各指标均差异不显著（P〉0.05）,说明乳酸菌与中药合生元可以替代抗生素饲喂给肉仔鸡.