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1.
The accumulation of soluble carbohydrates in vegetative parts of sweet sorghum ( Sorghum bicolor (L.) Moench.) represents an intermediate reserve pool for grain filling as well as an irreversible storage up to maturity. In order to study the effect of Sterility on soluble sugar accumulation in vegetative parts, two cytoplasmic male sterile (CMS) lines of sweet sorghum were compared with their corresponding fertile mamtainers for non-structural carbohydrate concentrations in shoots. Plants were harvested at four different physiological stages. The lines showed significant differences in the yield of non-structural carbohydrates. At maturity, the yields of soluble carbohydrates from vegetative parts of the two sterile lines were 93 % and 43 % higher than the yield of the two corresponding fertile lines. At that stage, sucrose represented about 80 % of total soluble carbohydrates and the higher yield of soluble carbohydrates of sterile lines was mainly due to the higher yield of sucrose. This indicates that the yield of extractable carbohydrates, especially sucrose, in vegetative parts of sweet sorghum could be considerably improved by using sterile lines which are utilized in hybrid production. 相似文献
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光敏核不育水稻选育与利用的几个问题讨论 总被引:4,自引:0,他引:4
对现有光敏核不育材料进行了分类,分为4种遗传类型;其中,温光弱感到光敏核不育系是最为理 想的遗传工具,并就其选育途径和选择“粳不籼恢”繁殖制种技术体系进行了论证. 相似文献
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以从美国威斯康星大学引进的叶用芥菜胞质雄性不育系叶用4-4为母本,以泰国清迈大学选育出的8个优良叶用芥菜品种为转育父本,杂交后连续回交4代(BC4),再将BC4与优良叶用芥菜品种40R2-3-4配制8个杂交组合,其中2个组合(4-4 × 19-H-12)×40R2-3-4和(4-4 × 2R2)×40R2-3-4结球率为100%,产量比商用品种分别高34%和13%,比其亲本分别高25%和5%。 相似文献
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小麦异源细胞质对主要病害抗性影响的初步研究 总被引:13,自引:0,他引:13
在大田自然发病和人工诱发条件下,研究了小麦异源细胞质对条锈、叶锈、秆锈、白粉和赤霉5种主要小麦病害抗性的影响。结果表明在同核条件下,不同异源细胞质对同一病害抗(感)性的影响不同,同一种细胞质对不同病害抗(感)性的影响也不完全相同;并且在不同地区试验的结果有时也有差异。根据初步研究结果,无芒山羊草、拟斯卑尔脱山 相似文献
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对两个玉米雄性不育系的细胞质分类研究 总被引:3,自引:0,他引:3
本文从普通遗传学、植物病理学和分子生物学的水平对玉米雄花不育系—元徐cms—小黄和恩二激cms—大黄进行了综合性的分类研究。大田的育性反应表明,这两个不育系的育性恢复受一对显性基因控制。对花粉染色的观察发现F1可育花粉与败育花粉基本上呈1:1分离。用小斑病T小种接种,这两个不育系不表现专化感染。通过mtDNA的分析 相似文献
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XU Jia-qi AN Mei-ling YUAN Yue HAO Yi-fan WANG Xiu-wen ZHANG Min WEI Xiao-wei LI Hua WANG Ren-jun 《园艺学报》2021,36(12):2148-2158
AIM To investigate whether microRNA-9-5p (miR-9-5p) mediates sympathetic overactivity by targeting KCNN3 (potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3) gene,which encoded small-conductance calcium-activated potassium channel 3 (SK3) protein, in paraventricular nucleus (PVN) of rats with type 2 diabetes mellitus (T2D). METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus (rAAV)-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control (DC) group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus (DM) rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced (P <0.05). However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3 . 相似文献