Determination of critical nitrogen dilution curve based on leaf area index for winter wheat in the Guanzhong Plain,Northwest China

Excessive use of nitrogen(N) fertilizers in agricultural systems increases the cost of production and risk of environmental pollution. Therefore, determination of optimum N requirements for plant growth is necessary. Previous studies mostly established critical N dilution curves based on aboveground dry matter(DM) or leaf dry matter(LDM) and stem dry matter(SDM), to diagnose the N nutrition status of the whole plant. As these methods are time consuming, we investigated the more rapidly determined leaf area index(LAI) method to establish the critical nitrogen(N_c) dilution curve, and the curve was used to diagnose plant N status for winter wheat in Guanzhong Plain in Northwest China. Field experiments were conducted using four N fertilization levels(0, 105, 210 and 315 kg ha-1) applied to six wheat cultivars in the 2013–2014 and 2014–2015 growing seasons. LAI, DM, plant N concentration(PNC) and grain yield were determined. Data points from four cultivars were used for establishing the N_c curve and data points from the remaining two cultivars were used for validating the curve. The N_c dilution curve was validated for N-limiting and non-N-limiting growth conditions and there was good agreement between estimated and observed values. The N nutrition index(NNI) ranged from 0.41 to 1.25 and the accumulated plant N deficit(N_(and)) ranged from 60.38 to –17.92 kg ha~(-1) during the growing season. The relative grain yield was significantly affected by NNI and was adequately described with a parabolic function. The N_c curve based on LAI can be adopted as an alternative and more rapid approach to diagnose plant N status to support N fertilization decisions during the vegetative growth of winter wheat in Guanzhong Plain in Northwest China.     

羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

八种中草药体外抑制大肠杆菌的效果观察

选取马尾松、田基黄、艾草、雷公根、芭蕉芯、浮萍、野菊花、番石榴叶8种中草药，采用常规中草药药敏试验打孔法和试管法对猪大肠杆菌（K88）、大肠杆菌（0157）进行体外抑菌试验。结果显示，艾草、野菊花、番石榴叶对大肠杆菌呈高度敏感，其中艾草对大肠杆菌抑菌力最强；马尾松挥发油对大肠杆菌呈中度敏感；田基黄、雷公根、芭蕉芯、浮萍对大肠杆菌无抑菌作用。     

蒸发浓缩－CODcr法测定地面水中低浓度COD

对水样蒸发浓缩法用于地面水中低浓度ＣＯＤ的测定进行探讨的实验结果表明，地面水中低浓度ＣＯＤ物质可以在碱性条件下，通过蒸发浓缩处理后，采用重铬酸钾法进行测定，所得结果的精密度和准确度良好．若选择水样浓缩系数为５，则可将重铬酸钾法的最低检测限浓度降低到１０ｍｇ·Ｌ－１，从而使该方法能够适用于地面水水质标准中各类水质ＣＯＤ的监测要求．     

Stoffbildung, CO2-Gaswechsel, Kohlenhydrat- und Stickstoffgehalt von Winterweizen bei erhöhter CO2-Konzentration und Trocken-streß

Dry Matter Production, CO₂ Exchange, Carbohydrate and Nitrogen Content of Winter Wheat at Elevated CO₂ Concentration and Drought Stress
Methods of mathematical modelling and simulation are being used to an increasing degree in estimating the effects of rising atmospheric CO₂ concentration and changing climatic conditions on agricultural ecosystems. In this context, detailed knowledge is required about the possible effects on crop growth and physiological processes. To this aim, the influence of an elevated CO₂ concentration and of drought stress on dry matter production, CO₂ exchange, and on carbohydrate and nitrogen content was studied in two winter wheat varieties from shooting to milk ripeness. Elevated CO₂ concentration leads to a compensation of drought stress and at optimal water supply to an increase of vegetative dry matter and of yield to the fourfold value. This effects were caused by enhanced growth of secondary tillers which were reduced in plants cultivated at atmospheric CO₂ concentration. Analogous effects in the development of ear organs were influenced additionally by competitive interactions between the developing organs. The content and the mass of ethanol soluble carbohydrates in leaves and stems were increased after the CO₂ treatment and exhausted more completely during the grain filling period after drought stress. Plants cultivated from shooting to milk ripeness at elevated CO₂ concentration showed a reduced response of net photosynthesis rate to increasing CO₂ concentration by comparison with untreated plants. The rate of dark respiration was increased in this plants.     

番茄对钾的吸收和分配的研究

对两个番茄品种，吸钾量的研究表明，“渝抗二号”番茄对Ｋ＾＋的吸收率较高，“早丰”番茄地上部分Ｋ＾＋的分配较多。当用ＮａＮＯ３代替营养液中的ＫＮＯ３后，Ｋ＾＋的分配比例与完全培养液中的植株相似，但分配到地上部的比例较小。加Ｎａ＾＋后，“渝抗二号”植株的叶和茎中的含Ｋ＾＋量比“早丰”更多，表明不同番茄品种在不同的Ｋ＾＋和Ｎａ＾＋环境中，有着明显不同的离子吸收和分配机制。     

Sensitivity of four Yeasts to Fungicides and CO2 Concentrations and Their Antagonistic Ability in Combination with Fungicide to pathogenic Fungi in vitro

The yeasts Cryptococcus laurentii,Trichosporon pullulans,Rhodotorula glutinis and Trichosporon sp.were used to investigate their sensitivity to four fungicides and different concentrations of CO2,as well as their antagonistic ability to Penicilliumexpansum and Alternaria alternata in vitro when applied with fungicide.There were significant differences in sensitivity to the fungicides among the different yeasts(P = 0.05).R.glutinis was more sensitive to Deccocil,Iprodione and Stroby as compared to other yeasts.Combination antagonistic yeasts with fungicide could more significantly enhance biocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05).C.laurentii was the most effective antagonist among the four yeasts and could completely control spore germination of P.expansum and A.alternata when combined with Stroby at the concentration of 100 μL L-1.The yeasts,except R.glutinis,could grow well on nutrient yeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp.showeda better adaptability to low temperature as compared to other antagonists.     

Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation

A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha^–1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha^–1 for the irrigated ones.Sodium and -amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg^–1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.     

THE CLIMATE CHANGE AND FORESTRY OF HEILONGJI ANG PROVINCE IN 1990''''S

INTRODUCTIONIntheworldoftoday,theglobalclimatechangeanditsinfluenceonecologyhavebe-comeaveryimportantproblem,towhichmanyscientists,governnentleadersandordinarypcoplepaycloseattentionI1-'].Inl979,theWorldClimateResearchProgram(WCP)waslaiddowninthefirstworldclimatemeeting.lnl99(),thesecondworldclimatemeetingwasconvcl1edinGencva,andalltl1eexpertsagreedthattheglobalwarmlngwillbeextremelyseriousdisasterthananynatUralcalamityever.Attl1eMectingof"WorldEnvironmentandDevelopment,"holdinBrazil…     

日光温室黄瓜栽培CO_2浓度的消长规律初探

近 3a(年 )的研究结果表明 :日光温室内CO2 浓度有明显的季节变化和日变化。在整个生长期内因通风时间和通风量的不同 ,日光温室内的CO2 浓度 11月和 3月较高 ,5月较低。各时期日变化基本相同 ,但变化幅度因季节而异 ,上午随Pn的逐渐增大而下降 ,中午 12 :0 0～ 14 :0 0时降至最低 ,下午又随Pn的减小而缓慢回升。叶片的光合作用、呼吸作用和土壤呼吸是影响日光温室内CO2 浓度日变化的主要因素。有机肥施用量对室内土壤呼吸和日光温室CO2 浓度有较大影响 ,在有机肥充足的条件下 ,室内CO2 浓度基本满足黄瓜光合作用的需要 ,无须补施 ,如果在作物生长期间再定时随水向土壤中冲施有机肥 ,效果就更好