The Effect of Detopping the Field Bean Plants (Vicia faba L. minor) and Lack of the Pod Setting on the Symbiosis with Root Nodule Bacteria

The plants of field bean var. Nadwiślaνski were detopped after about 1 month since the beginning of flowering and after that all flower buds were removed from some plants. The effect of a change in the trophic balance on the setting and subsequent growth of root nodules was studied.
The removal of pods had the greater effect on the increase in the weight of the remaining organs than the detopping of plants. Detopping retarded the ageing of the plants and delayed the phase of the greatest reduction of the number of pods. The detopping of plants revealed their considerable compensative possibilities in terms of production of the pod weight and the potential of leaves for biomass production. The data obtained indicate that plant detopping may disturb the root–bacteria exchange of signals, necessary to initiate the nodule development, while the further growth of nodules depends, above all, on the amount of available photosynthates.
A very high correlation between the dry weight of the whole plant to the dry weight of leaves ratio and the weight of nodules was observed. It proves the importance of the trophic balance of the plant for the root nodule weight production.     

Trichoderma harzianum produces nonanoic acid, an inhibitor of spore germination and mycelial growth of two cacao pathogens

An isolate of Trichoderma harzianum Rifai from an infected cacao pod produces and secretes nonanoic (pelargonic) acid into a liquid culture medium. Nonanoic acid (NA) was very inhibitory to spore germination and mycelial growth of two cacao pathogens, Crinipellis perniciosa Stahel and Moniliophthora roreri Cif. H.C. Evans. It was highly active causing 75% inhibition of spore germination in an in vitro assay at a rate as low as 0.09 μM for M. roreri and 0.92 μM for C. perniciosa. Mycelial growth was comparatively less sensitive to inhibition, but still there was a 75% reduction in growth with 0.62 μM in M. roreri and 151 μM NA in C. perniciosa. In contrast, NA did not affect Trichoderma mycelial growth or spore germination at concentrations that were inhibitory to the pathogens. 6-pentyl-α-pyrone was also produced and secreted into the medium by T. harzianum, however; it was not antagonistic to the cacao pathogens. Although a number of metabolites produced by Trichoderma spp. have been identified in the past, this is the first report of NA production and secretion by any Trichoderma. The results suggest that NA may play a role in the successful use of some Trichoderma spp. isolates in the biocontrol of fungal diseases of plants.     

Analysis of the seed dispersal patterns of wild soybean as a reference for vegetation management around genetically modified soybean fields

There are concerns that genetically modified soybean might threaten the genetic diversity of the wild soybean populations that are distributed in East Asia because genetically modified soybean has no crossing barrier with wild soybean. A simple and effective method to prevent hybridization via pollen flow is spatial separation between the two species because their hybridization occurs only when they grow in close proximity. Therefore, the invasiveness of wild soybean needs to be known in order to secure the appropriate distances. As wild soybean seeds are dispersed mechanically by pod dehiscence, an experiment was conducted in which white sheets were placed on the ground, concentric circles were drawn around the parent plants, and the number of dispersed seeds within each 0.5 m‐wide zone were counted. About 40% of the produced seeds were dispersed and the number of dispersed seeds gradually declined as the distance from the parent plants increased. The model that explained the relationship between the number and distance of the dispersed seeds was produced by using a generalized linear model procedure. More than 95, 99, and 99.9% of the produced seeds stayed within 3.5, 5.0, and 6.5 m after natural pod dehiscence. Knowing these values is useful for evaluating the level of invasive risk by mechanical seed dispersal. The goal of the work is to efficiently and deliberately prevent hybridization by isolating genetically modified soybean fields and wild soybean populations by vegetation management, including weeding and setting up specific‐width buffer zones.     

菜豆荚斑驳病毒免疫捕获一步RT-PCR检测

【研究目的】建立快速、准确、简便的检测大豆种子上菜豆荚斑驳病毒的免疫捕获一步RT-PCR方法(一步IC-RT-PCR)。【方法】采用抗原捕获和一步RT-PCR相结合的方法,并通过反应条件优化,确定一步IC-RT-PCR的反应程序,同时对该方法的特异性及实际检测效果进行验证。【结果】成功建立了一步IC-RT-PCR检测菜豆荚斑驳病毒方法,能够从感染菜豆荚斑驳病毒的大豆种子上扩增出预期大小的特异性片段,而对其他病毒及健康大豆无特异性反应;应用该方法对不同产地大豆种子样品进行检测,结果从来自美国的大豆样品中检出菜豆荚斑驳病毒,检出率为50%。【结论】一步IC-RT-PCR方法可以快速、准确地检测大豆种子上的菜豆荚斑驳病毒,适用于口岸检验检疫。     

Seasonal critical concentration and relationships of leaf phosphorus and potassium status with biomass and yield traits of soybean

Analysis of uppermost fully expanded leaves is useful to detect a deficiency of mineral nutrients such as phosphorus (P) and potassium (K) in soybean. Although, the leaf P or K status aids in fertilizer management, information on nutrient seasonal relationships with growth and yield traits at maturity are limited. To investigate this, soybean was grown under varying P or K nutrition under ambient and elevated CO₂ concentrations. Results show significant relationships of the relative total biomass and yield‐related traits with the foliar P and K concentrations measured several times in the season across CO₂ levels. However, the relationships established earlier in the season showed that the growth period between 25 and 37 d after planting (DAP), representing the beginning of flowering and pod, respectively, is the best for leaf sampling to determine the foliar P or K status. The leaf P and K status as well as the critical leaf P (CLPC) and K (CLKC) concentrations for traits such as seed yield peaked around 30 DAP (R2 stage) and tended to decline thereafter with the plant age. The CLPC and CLKC of seed yield indicate that the leaf P and K concentration of at least 2.74 mg g^?1 and 19.06 mg g^?1, respectively, in the uppermost fully expanded leaves are needed between 25 and 37 DAP for near‐optimum soybean yield. Moreover, the greatest impact of P and K deficiency occurred for the traits that contribute the most to the soybean yield (e.g., relative total biomass, seed yield, pod and seed numbers), while traits such as seed number per pod, seed size, and shelling percentages were the least affected and showed smaller leaf critical concentration. The CLPC or CLKC for biomass and seed yield was greater under elevated CO₂ 24–25 DAP but varied thereafter. These results are useful to researchers and farmers to understand the dynamics of the relationship of pre‐harvest leaf P and K status with soybean productivity at maturity, and in the determination of suitable growth stage to collect leaf samples.     

大豆抗食心虫性的遗传及抗虫育种方法研究——Ⅱ.开花期和成熟期与虫食率的关系

以一个营养生长期长的有限型大豆品系通交83—932与营养生长期短的无限型品系公交8324—19和亚有限型品种吉林27号杂交获得开花期和生育期广泛分离的F_2群体,在虫害大发生的条件下靠自然害虫群体在田间鉴定了亲本,F_1和F_2代的抗虫性,研究了开花期和成熟期对大豆抗虫性表现的影响。结果表明,三个组合F_2代单株的虫食率与开花期和成熟期均呈极显著负相关。开花期和虫食率间的关系较复杂,受多种因素的影响,可以用多项式曲线拟合。对一组正反交组合的研究表明,用营养生长期长的材料做母本时,F_2代低虫食率单株的频率显著高于其反交组合。在抗虫筛选中,按成熟期严格分组,在组内进行抗虫性选择有助于减少生育期的干拢,提高准确性。     

氮肥与密度互作对单粒精播花生根系形态、植株性状及产量的影响

为明确花生单粒精播适宜的氮肥水平和种植密度,本研究于2018年和2019年以‘花育22’为供试花生品种,设置3个氮肥水平(0 kg hm^–2, N0; 60 kg hm^–2, N1; 120 kg hm^–2, N2), 3个种植密度(7.93万株hm^–2, D1; 15.86万株hm^–2,D2; 23.79万株hm^–2, D3),采用二因素裂区试验设计,研究氮肥、密度及其互作对单粒精播花生根系形态、植株性状及产量的影响。氮肥对花生根长、根表面积、根体积、根干重的影响不显著,而密度的影响显著。单株根长、根表面积、根体积及根系干重随密度的增加而降低, D1显著高于D2和D3, D2、D3处理间差异不显著;单位面积根长、根表面积、根体积及根系干重随密度的增加而增加, D1显著低于D2和D3, D2、D3处理间差异不显著。氮肥和密度互作对2019年收获期单位面积根长、根表面积的影响显著,与D1相比, N1处理下D3的增幅显著高于N0和N2处理。氮肥及氮肥与密度互作...     

连作对花生幼苗生理特性及荚果产量的影响

池栽条件下研究了连作对花生幼苗生理特性及荚果产量的影响,结果表明,连作不仅影响植株干物质的积累,同时改变干物质的分配,根冠比增加;连作花生的叶面积、光合速率、叶绿素含量显著降低;连作同时影响磷、钾营养的吸收,但对其在植株中的分配影响不大;花生连作植株主茎高降低,单株结果数减少,荚果产量降低。增施磷钾肥,有利于花生连作的解除。     

基于结构方程模型的油菜性状和产量关系研究

为量化描述和分析甘蓝型油菜(Brassica napus L.)种植结构的调整以及相关性状变化对产量的影响,本研究利用结构方程模型对连续10年品种选育所得的168组产量和相关性状等数据进行分析,并对不同密度下相关性状和产量数据进行显著性分析。结果显示,在本实验密度范围内随着种植密度的增加,单株产量显著降低,且主要通过单株有效角果数影响单株产量,但总产量呈上升的趋势。研究所选各个性状对单株产量的总效应为:单株有效角果数>分枝数>千粒重>主花序有效角数>主花序有效长>每果粒数。因此,在培育更具有耐密性和对环境适用性的品种的同时,要选育拥有更多单株有效角果数和分枝数的品种以稳定单株产量,综合提高油菜总产量。     

不同施磷量对油菜新品种产量的影响

通过田间试验,研究磷肥与油菜产量、品质和植株形态之间的关系,确定油菜高产、优质、高效的磷肥施用量。不同油菜品种磷肥用量试验的结果表明:①每公顷施纯磷360kg的处理,中双9号,每公顷产量达3301.65kg,中油杂2号,产量达到最大值,每公顷产量3781.95kg。②一次分枝对产量影响最大,二次分枝、主花序的贡献相对要小得多。③随着施磷量的增加单位面积有效角果数呈增长的趋势。