植酸酶对3种植物性原料中植酸磷含量的影响

利用微生物植酸酶分别对3种植物性原料(豆粕、玉米蛋白粉、小麦)进行酶处理,研究不同浓度植酸酶作用下3种植物性原料中植酸磷含量的变化。3种原料分别有6组处理(植酸酶对照0 U/kg,柠檬酸对照,500U/kg,750 U/kg,1000 U/kg,1500 U/kg),每组处理3个重复。结果表明:植酸酶能够显著降低3种植物性原料中植酸磷的含量(P<0.05),同时提升有效磷的含量。通过对磷当量的分析,本试验中以植酸酶组1000 U/kg最为经济有效。植酸酶对照组中植酸磷含量也有所下降,说明柠檬酸单独对植酸起到了一定的分解作用。     

A major quantitative trait locus controlling phosphorus utilization efficiency under different phytate-P conditions at vegetative stage in barley

Organic phosphorus(P) is an important component of the soil P pool, and it has been proven to be a potential source of P for plants. The phosphorus utilization efficiency(PUE) and PUE related traits(tiller number(TN), shoot dry weight(DW), and root dry weight) under different phytate-P conditions(low phytate-P, 0.05 mmol L~(–1) and normal phytate-P, 0.5 mmol L~(–1)) were investigated using a population consisting of 128 recombinant inbred lines(RILs) at the vegetative stage in barley. The population was derived from a cross between a P-inefficient genotype(Baudin) and a P-efficient genotype(CN4027, a Hordeum spontaneum accession). A major locus(designated Qpue.sau-3 H) conferring PUE was detected in shoots and roots from the RIL population. The quantitative trait locus(QTL) was mapped on chromosome 3 H and the allele from CN4027 confers high PUE. This locus explained up to 30.3 and 28.4% of the phenotypic variance in shoots under low and normal phytate-P conditions, respectively. It also explains 28.3 and 30.7% of the phenotypic variation in root under the low and normal phytate-P conditions, respectively. Results from this study also showed that TN was not correlated with PUE, and a QTL controlling TN was detected on chromosome 5 H. However, dry weight(DW) was significantly and positively correlated with PUE, and a QTL controlling DW was detected near the Qpue.sau-3 H locus. Based on a covariance analysis, existing data indicated that, although DW may affect PUE, different genes at this locus are likely involved in controlling these two traits.