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《保鲜与加工》1997,(6):116-121
A review on environment-monitoring biosensors is presented.During recent years,various environment-monitoring biosensors are fast developed.They are applied to various areas,such as aquatic environment,atmosphere environment et al.The developing trend of environment_monitoring biosensors is also discussed. 相似文献
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Here we report the adaptation optimization of an efficient accurate inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified 《分子植物育种》2007,5(2):241-241
Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified (GM) crops and SN-P markers in model plant genomes. Briefly, aldehyde-attached sequence-specific singlestranded oligonucleotide probes are arrayed and covalently attached to a hydrazine-derivatized biosensor chip surface. Unique DNA sequences (or genes) are detected by hybridizing biotinylated PCR amplicons of the DNA sequences to probes on the chip surface. In the SN-P assay, target sequences (PCR amplicons) are hybridized in the presence of a mixture of biotinylated detector probes and a thermostable DNA ligase. Only perfect matches between the probe and target sequences, but not those with even a single nucleotide mismatch, can be covalently fixed on the chip surface. In both cases, the presence of specific target sequences is siL, nified by a color change on the chip surface (gold to blue/purple) after brief incubation with an anti-biotin IgG horseradish peroxidase (HRP) to generate a precipitable product from an HRP substrate. 相似文献
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表面等离子共振生物传感技术在食品安全快速检测中的应用研究进展 总被引:1,自引:0,他引:1
利用表面等离子体共振(SPR)生物传感技术进行食品的快速检测,具有无需标记、前处理简单、特异性好、灵敏度高以及能实时、连续监测生物分子动态反应过程等特点.详细论述了SPR生物传感技术在食品安全检测中的农药残留、细菌、病毒、重金属和糖类的快速检测的研究进展,并指出了其发展趋势. 相似文献
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检测鱼类新鲜度的常规方法是测定其总挥发性盐基氮(TVB-N)或细菌菌落总数,手续繁琐且代价较高,本文提出的方法是一种新颖的快速检测法。实验表明,在鱼的腐败过程中,总挥发性盐基氮(TVB-N,国标)、细菌落落总数和次黄嘌呤(Hx)的量之间皆存在着良好的相关性。因此,鱼腐烂而累积于组织的次黄嘌呤量可以指示鱼类的新鲜度,生物传感器快速检测法将取代现有的常规手段。 相似文献
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分子标记聚合物在生物传感器中的应用 总被引:1,自引:0,他引:1
胡晓娟 《河南农业大学学报》2001,35(4):387-390
介绍了分子标记聚合物(MIPs)具有与天然抗体同样的识别性能和与高分子同样的抗腐蚀性能的双重优点,广泛地被应用于生物工程、医药、环境监测、食品工业等众多领域,作者阐述了基质形状对分子标记或识别的影响,展望了其应用前景。 相似文献
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N-酰基-高丝氨酸内酯类群体感应信号分子检测方法的建立 总被引:1,自引:0,他引:1
N-酰基-高丝氨酸内酯(AHL)类化合物是革兰氏阴性菌群体感应系统中最重要的一类信号分子,并调控许多生理特性的表达。但由于AHLs分子为小分子物质,且AHL产生菌所产生的AHL的浓度极低,因此建立有效检测AHLs方法具有非常重要的意义。本研究利用两种细菌生物感应器Chromobacterium violaceum CVO26和Agrobacterium tumefaciens A136(pCF218/pCF372)建立了琼脂扩散法、薄层层析与细菌生物感应器相结合(TLC-Biosensor)、β-半乳糖苷酶活法等检测AHL的生物学方法,为研究革兰氏阴性菌的群体感应系统提供了有效手段。 相似文献
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为寻找快速、可靠的农药残留检测新方法,利用氧化石墨烯(GO)良好的水溶性分散碳纳米管(CNTs),一维(1D)的CNTs和二维(2D)的GO形成一种3D新型碳纳米复合材料,该复合材料具有疏松多孔的结构,能够有效地增大电极的比表面积。用该材料修饰玻碳电极,并固载乙酰胆碱酯酶(AChE)制备灵敏度较高、线性范围较宽的有机磷农药生物传感器。采用循环伏安法(CV)研究传感器的电化学性质。利用有机磷农药对AChE的抑制作用,以氯化乙酰胆碱为底物,对毒死蜱进行了检测。结果表明:生物传感器在最优条件下,0.71~71.13μmol/L范围内具有良好的线性关系,最低检出限为0.23μmol/L,且传感器的灵敏度、稳定性及重现性较好,适用于毒死蜱的残留跟踪检测。 相似文献
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Optimization of an oxygen-based approach for community-level physiological profiling of soils 总被引:1,自引:0,他引:1
María C. Zabaloy R. Michael Lehman Serita D. Frey Jay L. Garland 《Soil biology & biochemistry》2008,40(12):2960-2969
Current approaches for rapid assessment of carbon source utilization by whole soil communities (i.e., community-level physiological profiling or CLPP) provide a limited, biased view of microbial communities with little connection to in situ activities. We developed an alternative CLPP approach based upon fluorometric detection of dissolved oxygen consumption in a microtiter platform which offers flexible manipulation of experimental factors. In the attempt to reduce oxygen re-dissolution, the wells were filled with liquid to very near the top and sealed. We found that filling the wells with 240 vs. 150 μl of sample improved the sensitivity of the system to discern both the response to a substrate amendment as low as 10 mg l−1 and un-amended, endogenous respiration. The preparation of a soil slurry facilitates inoculation into the microplate. Disruption of soil samples had a limited effect on the endogenous respiration in comparison to intact soil microbags in a 24-well microplate. Storage time (up to 33 days) reduced the level of activity in intact soil microbags but not in disrupted samples. A microcosm fertilization experiment was set to study the effects of N availability on the respiratory response in the plates. The use of soil organic carbon (SOC) and amended C-substrates (50 mg l−1) was increased by the addition of nitrogen (N) in the plate, and appeared N-limited shortly after microcosm fertilization. The addition of the eukaryotic inhibitor cycloheximide delayed the initial increase in fluorescence (time to minimum response) of several C sources (casein, acetate, asparagine, coumaric acid), varying among soils, which could be explained by the fungal use of these compounds. However, the extent of the inhibition caused by cycloheximide did not increase at higher fungal to bacteria ratios as estimated by PLFA analysis, indicating that the direct estimation of the fungal biomass from cycloheximide addition is not feasible. This paper provides an optimized, standardized protocol for soil analysis, and sets the basis for further validation studies that will continue to define the underlying capabilities/biases of this approach. 相似文献