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大黄鱼发育进程中消化酶的活力变化 总被引:6,自引:0,他引:6
不同发育阶段大黄鱼(Pseudosciaena crocea)样品体重30~500g,取自厦门海区网箱养殖区。以酶学分析方法测定大黄鱼发育进程中胃蛋白酶、类胰蛋白酶、淀粉酶、脂肪酶4种消化酶的活力。结果表明,在大黄鱼发育进程中,对蛋白的整体消化能力始终较强;4种消化酶活力表现出2种变化模式,其中胃蛋白酶和类胰蛋白酶活力逐渐降低,而淀粉酶和脂肪酶活力呈上升趋势。胃蛋白酶、类胰蛋白酶、淀粉酶和脂肪酶的比活力随鱼体重的变化分别遵循回归方程Y=34.89/X 0.050;Y=53.36/X 0.29;Y=-22.38/X 1.13和Y=-1.35/X 0.13。初步认为,在大黄鱼发育过程中,150g体重为其生长发育的转折点,这一阶段各种消化酶的变化可反映其食性的变化情况,本研究旨为大黄鱼配合饲料的研制与开发提供基础依据。 相似文献
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探讨了反应温度、滴定酸度、胃蛋白酶添加量、CaCl2加入量等因素对胃蛋白酶凝乳作用的影响规律,确定了用胃蛋白酶生产干酪时的主要工艺参数,反应温度39℃;滴定酸度24T;CaCl2加入量0.015%;加酶量随酶活而定。 相似文献
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对体外胃蛋白酶法测定饲料氨基酸消化率与公鸡活体内测定结果进行了比较研究。体外酶法分析10种鸡饲料氨基酸消化率平均值分别是秘鲁鱼粉94.1%,受热损害秘鲁鱼粉84.4%,严重受热秘鲁鱼粉59.2%,淡鱼粉68.3%,肉骨粉72.2%,豆饼84.7%,花生粕78.5%,芝麻粕88.9%,脱毒棉籽饼63.9%,未脱毒棉籽饼70.7%。去盲肠公鸡活体法测定真消化率结果依次是:92.4%,84.2%,75.0%,77.2%,72.6%,86.8%,87.2%,88.9%,75.6%和76.1%。两者具有很高的相关关系,r=0.96,回归方程 Y=0.53+0.483X(Y=活体法结果,X=体外法结果)。结果证明:体外胃蛋白酶法测定饲料氨基酸消化率可以代替繁琐的鸡体内测定方法。 相似文献
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酪蛋白源阿片物质的分离、纯化及活性鉴定 总被引:15,自引:0,他引:15
酪蛋白的胃蛋白酶水解物通过RP-HPLC进行分离,并用NG108-15 细胞对其进行阿片样活性鉴定。实验结果表明:酪蛋白经胃蛋白酶处理2 h 后, 其酶解产物中有阿片样活性物质产生, 而酪蛋白本身无阿片活性。 相似文献
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甲醛处理对豆饼干物质和蛋白质瘤胃降解率及胃蛋白酶消化率的影响 总被引:3,自引:1,他引:2
豆饼用2g/kgDM甲醛处理后,10h瘤胃干物质(DM)降解率从87.19%下降到豆饼含水量为14%和60.93%和含水量18%时的56.29%,粗蛋白降解率从87.69%分别下降到48.36%和43.43%,随着甲醛用量的增加,干物质的粗蛋白的降解率进一步下降,但下降幅度减少。在甲醛用量低于2g/kgDM的情况下,甲醛用量对PH为4.5时的胃蛋白酶4h和8h干物质和粗蛋白消化率没有明显影响,但 相似文献
8.
Hongyue Zhao Dong Han Shouqi Xie Xiaoming Zhu & Yunxia Yang 《Aquaculture Research》2009,40(16):1864-1872
The present study was carried out to investigate the influence of water temperature on the growth performance and digestive enzyme (pepsin, trypsin and lipase) activities of Chinese longsnout catfish. Triplicate groups of Chinese longsnout catfish (35.6±0.48 g, mean±SE) were reared at different water temperatures (20, 24, 28 and 32 °C). The feeding rate (FR), specific growth rate (SGR) and feed efficiency ratio (FER) were significantly affected by water temperatures and regression relationships between water temperature and FI, SGR as well as FER were expressed as FR=−0.016 T 2 +0.91 T −10.88 ( n =12, R 2 =0.8752), SGR=−0.026 T 2 +1.39 T −17.29 ( n =12, R 2 =0.7599) and FER=−0.013 T 2 +0.70 T −8.43 ( n =12, R 2 =0.7272). Based on these, the optimum temperatures for FR, SGR and FER were 27.66, 26.69 and 26.44 °C respectively. The specific activities of digestive enzymes at 24 or 28 °C were significantly higher than that at 20 or 32 °C. In addition, there was a significant linear regression between FR or SGR and specific activities of pepsin and lipase, which indicated that pepsin and lipase played important roles in regulating growth through nutrient digestion in Chinese longsnout catfish. 相似文献
9.
Suktianchai Saekhow Harit Sae‐khoo Karun Thongprajukaew Anida Songnui Wutiporn Phromkunthong 《Aquaculture Research》2018,49(5):1887-1895
Bigfin reef squid (Sepioteuthis lessoniana) is an economically important exported aquatic animal in Thailand, but little is known about its husbandry, which needs to be clarified. In this study, the ontogenic development of the main digestive enzymes (pepsin, trypsin, chymotrypsin, lipase and amylase) was investigated within 30 days after hatching (DAH). Fertilized eggs of the wild cuttlefish were hatched and then distributed into four replicate aquaria assigned to varying collection times (0, 5, 10, 15, 20, 25 and 30 DAH). The development of pepsin suggests acidic digestion functionality at 5 DAH, and its specific activity was maintained over the studied period. Specific activities of the serine proteases, trypsin and chymotrypsin, fluctuated significantly with similar pattern, and appear to be the key enzymes contributing ontogenic changes in protein catabolism. The specific activity of lipase was relatively high at 0 DAH, dramatically increased by 5 DAH, and then appeared constant until the end of observations, suggesting important role in utilizing the lipid from yolk at hatching, as well as probably in sparing protein utilization. Dramatic increase in amylase specific activity occurred by 10 DAH after which the activity was constant until 30 DAH, suggesting the utilization of carbohydrate as energy evolves comparatively late. Findings from the current study could be used to manage nutritional protocols according to the digestive enzyme patterns, as well as to develop artificial diets for rearing S. lessoniana. 相似文献
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琥珀酸对水貂胃蛋白酶活性、营养物质消化率及生产性能的影响 总被引:1,自引:1,他引:0
将健康、体重相近的雄性短毛黑水貂60只,随机分成4组,每组15只.在相同基础鲜料中添加0%、0.2%、0.4%、0.8%的琥珀酸,在冬毛生长期进行75 d饲养试验,记录水貂采食量,收集粪便测定营养物质消化率;结合屠宰取皮测定水貂胃蛋白酶活性和皮长、毛绒品质等生产性能指标.结果表明,各处理间胃蛋白酶活性差异显著(P<0.... 相似文献