THE INSENSITIVITY OF 99mTc PERTECHNETATE FOR DETECTING METASTASES OF A FUNCTIONAL THYROID CARCINOMA IN A DOG

This report describes the use of ^99mtechnetium pertechnetate (^99mTcO₄^−) and ¹³¹[for imaging of a metastatic thyroid carcinoma in a dog. The ¹³¹] imaging showed metastatic lesions undetected by the ^99mTcO₄^− imaging on 2 separate occasions. The possible mechanisms for the discrepancies between ¹³¹I and ^99mTcO₄^− imaging of thyroid carcinomas are discussed. The use of ¹³¹I for the imaging of functional thyroid carcinomas in the dog is recommended.     

Different biological behaviour of Waldenström macroglobulinemia in two dogs

Waldenström Macroglobulinemia is a low‐grade immunosecretory disorder associated with lymphoid tumours, which is rarely reported in veterinary medicine. In this study, we describe two clinical cases of this rare syndrome in dogs, each characterized by a different onset and clinical course. In one case, a hyperacute onset and aggressive behaviour of the neoplasm was observed. Absolute serum viscosity (SV) was retrospectively evaluated in order to explain clinical findings. Rotational viscosimetry showed good precision in measuring SV. Both dogs had SV values higher than a control groups of healthy dogs although only one subject developed hyperviscosity symptoms and complications. At high paraprotein concentrations, a slight reduction of the M‐component was associated with a marked decrease in SV. Thus, this work suggests that SV assessment is a relevant tool for managing monoclonal gammopathies, whose usefulness should be further confirmed in larger cohorts of dogs.     

m6A甲基化在鸡肌肉生长发育中的表达研究

试验旨在研究RNA m6A修饰相关基因去甲基化酶Alk B同源蛋白5（Alk B homologue 5，ALKBH5）、去甲基化酶肥胖相关蛋白（fat mass and obesity-associated protein，FTO）、甲基转移酶样蛋白3（methyltransferase like 3，METTL3）、甲基转移酶样蛋白14（methyltransferase like 14，METTL14）和成肾细胞瘤1-结合蛋白（Wilms’tumor 1-associating protein，WTAP）在鸡骨骼肌发育过程中的表达，分析其与骨骼肌m6A甲基化水平的相关性。首先，利用实时荧光定量PCR技术检测m6A甲基化相关基因在金茅花鸡12（E12）、14（E14）、16（E16）、18（E18）胚龄和1日龄腿肌和胸肌组织中mRNA表达水平，以及其在鸡成肌细胞50%、100%增殖期和1、2、3、4、5 d分化期的mRNA表达水平；随后，利用m6A甲基化试剂盒检测金茅花鸡E12和1日龄腿肌和胸肌组织中m6A甲基化修饰水平，与m6A甲基化相关基因表达水平进行相关性分析。结果显示，m6A去甲基化基因ALKBH5和FTO mRNA表达水平在骨骼肌发育过程中显著上调（P<0.05），即在E12、E14低表达，E16、E18逐渐上调，1日龄达到最高。m6A甲基化写入基因METTL14、METTL3和WTAP mRNA表达水平在E12、E14、E16逐渐上升，E18下降，随后至1日龄表达量回升。在细胞增殖过程中，ALKBH5、FTO、METTL14、METTL3和WTAP基因表达均上调；在细胞分化过程中ALKBH5和FTO基因表达水平显著上调（P<0.05），在分化第5天达到最高。METTL14、METTL3和WTAP基因mRNA表达水平在细胞诱导分化的1、2、3、4 d表达量呈下降趋势，而在诱导分化的第5天有所回升。甲基化水平检测结果显示，腿肌和胸肌m6A甲基化水平变化趋势一致，均在胚胎发育过程中显著下降（P<0.05），至1日龄达到最低。相关性分析结果显示，鸡骨骼肌RNA m6A甲基化水平与m6A去甲基化修饰基因ALKBH5、FTO mRNA表达水平呈显著负相关（P<0.05）。综合以上试验结果，推测m6A甲基化修饰与鸡骨骼肌发育相关，而去甲基化基因ALKBH5、FTO可能通过调控RNA m6A甲基化水平，影响鸡骨骼肌发育。本研究结果为进一步研究m6A甲基化修饰调控鸡骨骼肌生长发育的功能和分子机制提供理论依据。     

利用农田表层(0～20cm)有机碳估算1m土体有机碳的可靠性

<正>土壤有机碳(SOC)是近年来全球变化研究的热点之一,表层(0~20cm)和1m土体的SOC是评估农田SOC的两个重要指标[1]。很多研究利用第二次土壤普查资料来估算农田SOC[2-6],一些研究已表明近20年来我国耕作土壤,尤其是表层(一般为0~20cm)的SOC基本呈增加的趋势[5-8],这与秸秆还田、有机肥与化肥合理施用以及少(免)耕技术推广有关[9-10]。但由于农田SOC,至少是表层SOC,是     

基于二分法的微灌毛管水力设计

根据微灌毛管水力学特性,采用二分法搜索原理,并与逆递推法相结合,提出微灌毛管水力解析与设计方法。利用计算机编程从毛管末端向毛管进口逆序递推,可以快速、方便地求解出毛管各个孔口的压力和流量,设计毛管运行的水力参数,校核平均流量、流量偏差率等设计控制指标。实例验证结果表明,该设计方法具有直观、简便和快捷的特点,易为非专业人员理解与应用,具有较高的求解效率和计算精度。     

抗伤寒杆菌Fe—SOD血清对志伤寒杆菌攻击小鼠的免疫保护作用

目的：制备兔抗伤寒杆菌Ｆｅ－ＳＯＤ血清,并研究其对鼠伤寒杆菌攻击小鼠的免疫保护作用,探讨ＳＯＤ与沙门氏菌致病性的关系。方法：用纯化的伤寒杆菌Ｆｅ－ＳＯＤ免疫家兔,制备兔抗ＳＯＤ血清,并用ＥＬＩＳ法检测抗血清效价;免疫电泳、双向琼脂扩散试验和抗血清对酶活笥抑制试验检测抗血清的特异性。将所制备的抗血清与鼠伤寒杆菌混合,放３７℃３０ｍｉｎ,经腹腔注入小鼠体内,并观察３天和７天小鼠的存活情况,以确定抗血清     

超级稻甬优12产量1000kg/667m~2手插栽培技术初步集成

以甬优12在居于5 300℃积温线的浙江中部新昌县的试验实践为基础,提出了1 000 kg/667 m2手插栽培的基本技术思路、群体生育指标及主要集成技术。     

大豆主要过敏原Gly m Bd30K蛋白单克隆抗体的制备与应用

利用大豆主要过敏原Gly m Bd 30K蛋白抗原表位蛋白为免疫原免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤NS-1细胞融合.采用半固体培养基法和有限稀释法相结合的方法快速筛选获得稳定分泌的特异性杂交瘤细胞,用杂交瘤细胞株诱生小鼠腹水,应用蛋白A亲和层析法进行抗体纯化.采用Ig类与亚类鉴定试剂盒鉴定该单克隆抗体...     

Determination of primary structure of amberjack myosin heavy chain and its relationship with structural stability of various fish myosin rods

The structural stability of fish myosin depends upon species and temperatures of water in which fish live. Primary, secondary, and quaternary structures of myosin heavy chain (MyHC) from three species of fish living at different temperature ranges have been compared with those of rabbit MyHC in order to investigate the differences in stability. Primary structure of MyHC, although being accessible for warm-water and cold-water fish (carp and walleye pollack), was not available in previous for tropical-water fish literature; so in this study primary structure of MyHC of the tropical-water fish amberjack has been determined by cloning and sequencing its cDNA. The MyHC has 1938 amino acid residues (AA), which are almost as much as as those of carp and walleye pollack. The amberjack MyHC is 91&amp;#x2013;95% homologous with other fish and rabbit MyHCs. There is a discernible difference between animal species with stable myosin rod (amberjack, carp, and rabbit) and walleye pollack with unstable rod. Stable rod species have a high probability of forming coiled-coil around the COOH-terminal end of the rod, while the pollack has a low coiled-coil formation probability. In addition, the average scores of the coiled-coil for myosin rod were rabbit (1.738) > amberjack (1.691) > carp (1.680) > walleye pollack (1.674) which correlated exactly with the observed stability. The results suggest that coiled-coil forming ability, particularly around the COOH-terminal end, directs structural stability of fish myosin rod.     

早熟晚粳机插秧700kg/667m~2精确定量栽培技术规程

通过对早熟晚粳稻品种武运粳19号及武2645新品种的引种试验和示范,将"机插育壮秧、控行缩株适苗、麦草全量还田、精确定量施肥"等技术进行集成,制订了早熟晚粳机插精确定量栽培技术规程,以期为大面积机插秧实现高产提供技术支撑。