治疗图书馆顽症:流程重组

本文将流程重组理论引入图书馆管理，分析了图书馆流程重组的必要性，同时具体阐述了图书馆流程重组的三种方式。     

复合图书馆的信息服务探讨

复合图书馆作为图书馆的一种发展形态，有其自身的特点和要求，有许多值得研究的内容。复合图书馆的读者服务工作是图书馆工作的出发点和各项业务工作的最终价值体现。文章分析了目前图书馆读者服务工作面临的形势，探讨了我们应采取的措施。     

图书馆业务外包的实施策略

业务外包是一种新型的图书馆管理方法。本文介绍了图书馆业务外包的概念、内容、作用，探讨了实施图书馆业务外包的相应策略。     

浅谈职业技术院校的图书馆管理

论述了职业技术院校图书馆的定位、服务群体、困境等现状,并在此基础上对职业技术院校图书馆的发展进行了探讨。     

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay

BackgroundASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection.ObjectiveTo identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays.MethodWe used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide.ResultsThe results of our prediction revealed that the possible antigen epitope regions were A23–29, A36–45, A72–94, A114–120, A124–130, and A137–150. The indirect ELISA showed that the peptides A23–29, A36–45, A72–94, A114–120, and A137–150 have good antigenicity. Moreover, the A36–45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44.ConclusionsOur study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.     

图书馆岗位匹配度的研究

论述了新世纪图书馆岗位匹配的含义和原则,并根据当前图书馆人岗不匹配的现状和问题,对图书馆人与岗位的合理匹配作出了具体而深刻的阐述。     

图书馆员在高校图书馆建设中的主导作用

阐述了图书馆员在高校图书馆基础设施、馆藏文献、文化、服务、管理、教育、研究等方面建设中的主导作用。     

关于读者隐性伤害的法律思考

就读者隐性伤害的表现,分析了图书馆相关的法律责任,并提出了防范和解决读者隐性伤害的途径。     

形象信息传播--图书馆形象塑造的重要保障

形象信息的传播是图书馆形象塑造成功的重要保障。文章就图书馆形象信息传播的措施进行了具体地论述．并提出应重视形象信息传播过程中的扩散效应和放大效应。     

基于OPAC日志的读者需求分析

OPAC日志中详细记录了读者的访问活动,通过对OPAC日志的深入分析,可挖掘出读者的各种需求信息。为了更好地了解读者的需求。实现对图书馆决策的支持,本文以汇文的OPAC系统为例深入研究了OPAC日志分析的方法和过程。并给出了具体的实现方法、分析实例和结果。