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1.
脑活素治疗新生大鼠缺血缺氧性脑病机理的研究 总被引:1,自引:0,他引:1
7日龄Wister大鼠结扎左侧颈总动脉后吸入氧氮混合气体(8%O2和92%N2)2h,制成缺血缺氧性脑病动物模型,测定大鼠脑组织中丙二醛(MDA)的含量和超氧化物歧化酶(SOD)的活性。结果显示,缺血缺氧后6h,缺血缺氧组MDA含量显著升高,24h达到高峰,以后逐渐下降,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组MDA含量于缺血缺氧后6h已经显著下降,72h与对照组比较无统计学差异。缺血缺氧组SOD活力值在缺血缺氧后6h已经下降,24h降到最低点,以后逐渐回升,96h与对照组比较无统计学差异;缺血缺氧脑活素治疗组SOD活力值在缺血缺氧后6h明显提高,于缺血缺氧后96h与对照组比较无统计学差异。结果提示,缺血缺氧引起新生大鼠脑组织的氧化-抗氧化系统失衡,氧自由基大量产生,参与了新生大鼠缺血缺氧性脑病脑损伤过程;脑活素能够抑制氧自由基的生成,提高SOD的活力值,对缺血缺氧性脑病具有神经保护作用。 相似文献
2.
阐述了人工引雷技术的原理,结合2008年夏季在大兴安岭松岭地区进行的人工引雷实验情况,具体介绍了利用火箭拖带细金属导线进行人工引雷的专门技术,包括场地布局、所采用的技术手段及部分试验结果。通过电场变化的同步测量和光学观测,揭示了人工引雷的放电特征与自然闪电有极大的相似性,提出了人工引雷技术在研究雷击效应和引燃林火机理方面的具体应用以及主动防御森林雷击火灾的潜力,探讨了近距离同步测量云地闪电回击电流、量化分析雷击火机理的物理模式。 相似文献
3.
SUN Kai CHEN Wen-hua ZHANG Ying LI Yan AN Meng-yao PAN Ying-ying WU Yan-na KANG Yi GAO Wei-zhen LOU Jian-shi 《园艺学报》2017,33(1):116-122
AIM: To study the effects of noninvasive delayed limb ischemia preconditioning (NDLIP) on animal cardiac function, myocardial morphology and myocardial apoptosis after myocardial infarction (MI). METHODS: Healthy SD male rats[n=45, weighing (250±10) g] were randomly divided into 3 groups:MI group:the animal model of MI was established by surgical ligation of left anterior descending artery (LAD) after 2 weeks; NDLIP group:after the success of the MI animal model, NDLIP was carried out every other day until the 4th, 6th and 8th weeks; sham group:as the negative control group, the animals were taken heart LAD threading but no ligation. All rats were fed conventionally. At the end of the 4th, 6th and 8th weeks, all rats were made ventricular intubation, and then the hemodynamic parameters were recorded. The blood samples were withdrawn from the abdominal aorta and the serum was separated via centrifugation. The serum contents of Bcl-2 and Bax were measured by ELISA. Left ventricular anterior wall was homogenized. The mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ and Ⅳ in the myocardial tissues were detected by ELISA. RESULTS: At the end of the 4th, 6th and 8th weeks, compared with MI group, left ventricular systolic pressure in NDLIP group was significantly increased, while left ventricular end-diastolic pressure in NDLIP group was significantly decreased (both P<0.05). Mitochondrial respiratory chain complexesⅠ, Ⅱ, Ⅲ and Ⅳ in NDLIP group were significantly increased (P<0.05). The serum level of Bcl-2 in NDLIP group was significantly increased and Bax level was reduced remarkably (both P<0.01). CONCLUSION: NDLIP improves the hemodynamic indexes, promotes the mitochondrial respiratory function and inhibits cell apoptosis, thus improving the prognosis of MI. 相似文献
4.
Byeong-Teck Kang Jong-Hwan Lee Dong-In Jung Chul Park Su-Hyun Gu Hyo-Won Jeon Dong-Pyo Jang Chae-Young Lim Fu-Shi Quan Young-Bo Kim Zang-Hee Cho Eung-Je Woo Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):369-376
The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients. 相似文献
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6.
目的探讨诱导PC12细胞分化的神经细胞靶向沉默Smad7基因特性,同时进行沉默效果检测.方法以Smad7基因为靶目标,设计合成3条siRNA序列,进行细胞转染,利用Rea1time—PCR和Westernblot技术检测沉默效果,筛选出最有效的干扰序列,同时检测出最佳的转染浓度和转染时间.结果针对Smad7基因设计合成及筛选出靶向沉默Smad7基因的干扰序列(siRNA1);siRNA1的最佳转染浓度是4μg/mL;siRNA1的最佳转染时间是24h;siRNA1对Smad7的抑制效果优于其他干扰序列.结论siRNA1能有效沉默Smad7基因;lipofecta—mineTM2000可成功将siRNA1转染至神经细胞,转染效率较高;利用siRNA技术能有效抑制神经细胞. 相似文献
7.
流刺网是影响船舶航行安全的因素之一,本试验利用渔船的冲程使渔船冲过流刺网,从而使渔船驾驶员掌握渔船航行中避免螺旋桨被流刺网绞缠的方法,确保渔船航行生产的安全。 相似文献
9.
AIM:To observe the therapeutic effect of Shuxuetong combined with atorvastatin for treating transient ischemic attack(TIA). METHODS:We retrospect 46 cases of TIA patients from August 2009 to December 2010 in our hospital treated with Shuxuetong combined with atorvastatin. The clinical data such as coagulation function, blood lipid, fasting blood glucose and the carotid color Doppler ultrasonic examination before and after treatment were collected. All patients continued to take atorvastatin for 6 months and were followed up for 6 to 12 months. RESULTS:The total effectiveness of using Shuxuetong combined with atorvastatin for TIA in 46 cases was 91.30%. Five patients were found to suffer from recurrent TIA or the occurrence of stroke in the cured or effective 42 cases during follow-up, the incidence of which was 11.90%. Six months after treatment, low-density lipoprotein-cholesterol(LDL-C) and triglycerides(TG) were obviously lower than those before treatment(P<0.05), and the carotid intimal thickness(IMT) and the plaque area were decreased as compared with those before treatment(P<0.05). CONCLUSION:Treatment with Shuxuetong+atorvastatin for TIA has good therapeutic effect and reduces the recurrence of TIA and stroke incidence, which may be related to the reduction of LDL-C and TG levels, IMT and the plaques area. 相似文献