排序方式: 共有41条查询结果,搜索用时 15 毫秒
1.
XU Wan-yun WANG Hui-min WANG Wen-lun HU Meng-wei YAN Guo LI Jian-hua GAO Jian-feng 《中国畜牧兽医》2016,43(3):568-576
The specific primers were designed according to Ovis aries DRA gene sequence deposited in GenBank and the multiple cloning site of the plasmid pYD1,which was a vector used for protein surface display on Saccharomyces cerevisiae.The gene encoding DRA was amplified by PCR using the genomic RNA of Ovis aries.The 762 bp fragment was cloned and released in GenBank and registration number was KR422362.The PCR product was inserted into the yeast surface display plasmid vector pYD1 by double enzyme digestion.It was indicated that DRA gene was successfully integrated into the genome.Dot mutation was made at both ends of exon 2 in DRA gene for making restriction enzyme cutting site and design the exon 2 specific primers according to mutated Ovis aries DRA gene sequence.Sequenced exon 2 amplification products based on DNA pooling of sheep large sample template was analyzed the polymorphic loci.The polymorphic exon 2 246 bp fragment was obtained by double enzyme digestion and connected to surface display restructuring mutation carriers pYD1-DRA by the same double enzyme digestion,and then we successfully constructed yeast surface display libraries.We transformed it into Saccharomyces cerevisiae EBY100 cell.Yeast monoclone was identified by PCR amplification and sequencing,and we confirmed that DRA gene had been integrated into Saccharomyces cerevisiae genome.After galactose induced,it was detected that DRA gene library had been successfully demonstrated on the yeast cell surface under the fluorescence microscope by immunofluorescence method. 相似文献
2.
五指山猪近交系群体中DRA和DRB基因的SSCP检测 总被引:10,自引:0,他引:10
为确定五指山猪(WZSP)近交系群体SLA!ⅡDRA、DRB基因的等位基因数及其特性,利用PCR扩增SLA!ⅡDRA、DRB基因的第2外显子序列,经单链构象多态性即SSCP检测其等位基因数,选择纯合子个体的PCR产物直接测序。对所获得的序列与Genbank中所有相应的序列进行比对和聚类分析,特别是SLA!DRB!C、SLA!DRB!D、HLA!DRB*09012、HLA!DRB*1201、HLA!DRA*0101等位基因。结果表明:在WZSP近交系群体中,DRA、DRB各有2个等位基因且发现1个DRB新等位基因。DRA第2外显子有很强的保守性,而DRB基因呈现出高度的多态性,在核苷酸水平同源性为85%以上,氨基酸水平同源性为70%以上。该试验成功地检测了WZSP近交系SLA!DRA、DRB基因的等位基因及其特性,为建立WZSPSLA!DR基因抗原的分子分型及特异单倍型猪的培育研究奠定基础。 相似文献
3.
4.
单宁用于带锈钢铁防蚀处理的研究 总被引:3,自引:1,他引:2
用栲胶及其增纯产物和相应的单宁-铁螯合物代替工业单宁酸成功地用于带锈钢铁的抗蚀处理,研究了处理剂的配方、配制方法,并对其性能进行了测试,指标均达到或超过了工业单宁酸。 相似文献
5.
6.
DRD1及DRD2基因多态性与鸡冠高和体重的相关性 总被引:1,自引:0,他引:1
研究鸡DRD1及DRD2基因与冠高和体重的关系。在DRD1和DRD2基因上共选取20个多态位点,对586只宁都三黄鸡的基因型进行检测,分析各位点与77、84及91日龄冠高和体重性状的相关性。结果表明:位于DRD2基因5′侧翼区的位点A-6543G与91日龄体重显著相关(P<0.05),位点C-6539T与91日龄冠高显著相关(P<0.05);DRD1基因编码区上6个多态位点与不同日龄的鸡冠高和体重无显著相关(P>0.05),但由C+765T、C+1011T与G+1065A这3个位点组成的单倍型块与77日龄冠高呈显著的相关性(P<0.05)。研究结果提示:DRD1基因的单倍型块可作为77日龄冠高的辅助选择的有效分子标记;而DRD2基因上A-6543G可作为91日龄体重的有效分子标记,位点C-6539T可为91日龄冠高标记辅助选择提供参考。 相似文献
7.
利用烷基醇与五氧化二磷反应生成烷基磷酸单酯,使其进一步与烷基硅氧烷反应,得到烷基链分别为12个碳和18个碳的六元环烷基硅氧烷-磷酸酯化合物,作为天然气管道缓蚀型减阻剂.其减阻机理包括管道壁面粗糙度降低而减阻及弹性分子膜产生移动波而减阻.在50℃和质量分数为5%的NaCl中性溶液腐蚀条件下,通过静态挂片失重法测得烷基链为12个碳时缓蚀效率为86.7%,烷基链为18个碳时缓蚀效率为83.9%;通过模拟环道测得烷基链为12个碳时减阻率为10.6%,烷基链为18个碳时减阻率为11.2%. 相似文献
8.
以络合型TiC13催化剂、N型Ziegler-Natta催化剂和[O,O,N,N]茂金属为主催化剂,采用本体聚合方法,制备了单峰和双峰聚α-烯烃油品减阻剂,考察了催化剂的种类与用量对聚α-烯烃分子质量、分子质量分布及减阻剂性能的影响.结果表明:3种催化剂中,[O,O,N,N]茂金属催化剂的催化效率最高,制备的聚α-烯烃分子质量、分子质量分布及减阻性能最优;使用双金属络合型TiC13和[O,O,N,N]及N型Z-N和[O,O,N,N]制备的双峰聚α-烯烃减阻剂的起效速度和减阻效果均优于单峰聚α-烯烃减阻剂. 相似文献
9.
10.