Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.     

Comparison of viral RNA populations of pathogenically distinct isolates of Citrus tristeza virus : application to monitoring cross-protection

The population of sequence variants of Citrus tristeza virus (CTV) isolates of different geographic origins and pathogenicity properties was characterized by single-strand conformation polymorphism (SSCP) analysis of cDNA of the genes p18, p13, p20 and p23. The mild isolates analysed here usually yielded a SSCP profile with two DNA bands, suggestive of a predominant sequence variant, whereas the SSCP profile of the most virulent isolates contained more than two DNA bands, indicating that their viral populations are likely to be more complex. The set of SSCP profiles of the four genes allowed identification of individual isolates, but no profile characteristic of a geographic area or a biogroup was found. Sweet orange plants singly inoculated with a mild or with a severe isolate yielded the SSCP profile characteristic of each isolate, whereas the SSCP profile of plants successively inoculated with both isolates was a composite of the two individual profiles. The SSCP profile of plants singly inoculated remained constant, but the profile of doubly inoculated plants varied with time. Plants in which the SSCP profile of the severe isolate became predominant showed stem pitting, and those in which the predominant profile corresponded to the mild isolate remained symptomless. The results indicate that SSCP analysis can be used to study changes in RNA populations of doubly inoculated plants and to monitor cross-protection between mild and severe isolates.     

部分军队医学期刊论文关键词标引质量的调查

 

以中国生物医学文献数据库(CBMdisc)的主题标引为金标准，

对数据库中用主题词标引的23

种军队医学期刊，共计265篇医学论文关键词标引进行对比分析。发现其中存在4个方面问题：一是标引质量问题；二是主、副主题词组配错误；三是将缺乏检索意义的形容词、副词及专业上未得到公认和未被普遍使用的缩略词用作关键词；四是关键词数量普遍不足。为提高关键词标引质量，应重视关键词标引工作的意义，切忌随意性、潦草对待;配备最新的标引工具书;标引工作严格遵循标准化程序，提高整个行业的质量水平。

     

钢球滚子弧面分度凸轮载荷分布

运用空间啮合原理和回转张量法,推导出凸轮滚道截形采用双圆弧结构时,钢球滚子弧面分度凸轮的廓面方程和啮合方程.应用赫兹接触理论和变形协调条件,推导出载荷在每个参与啮合钢球中的分配关系.通过实例计算,发现每个钢球上的载荷大小分配不同,在钢球的啮入、啮出点,钢球受力发生突变,参与啮合的钢球数目越多,每个钢球的受力越小.     

Where Is Agricultural Economics and AgriBusiness Research Information Published and Indexed? A Comparison of Coverage in Web of Knowledge,CAB Abstracts,EconLit, and Google Scholar

ABSTRACT

Identifying the best source of information to satisfy the needs of local clientele has always been the challenge facing collection development and instruction librarians. In order to provide users with the best possible access tool and source for comprehensive information, it is important that librarians be aware of the most productive sources of information in a field. This paper identifies where the bulk of agricultural economics research is published and indexed. It also ascertains whether Google Scholar is as productive in covering this information as CAB Abstracts and EconLit. The cited reference counts for the top 50 cited sources in Web of Knowledge and Google Scholar are also compared. The scatter of the journal literature in this field, based on the Library of Congress Subject Headings, is also provided. The study identified a broad range of article scattering in areas where agricultural economics and agribusiness materials are published. On the cited reference count, Google Scholar was as productive as the Web of Knowledge. Google Scholar is a free source of very useful information for cited references and other subject searches in the area of agricultural economics and could be used to complement traditional databases.     

标准文献的CNMARC格式著录和标引

标准文献与普通图书相比有许多不同之处。主要介绍了我国标准文献的概况、特征、主要著录项目及信息源。用实例探讨了标准文献特有的CNMARC著录方法及标引。     

牧草抗旱性综合评价指标体系的AHP模型设计与应用的研究

在查阅大量牧草抗旱性鉴定指标研究资料的基础上,应用层次分析法（ＡＨＰ模型,ＡｎａｌｙｔｉｃＨｉｅｒ－ａｒｃｈｙＰｒｏｃｅｓｓ）理论,根据专家咨询系统的反馈信息,对所选择的６类抗旱性鉴定指标及其２１个指标变量,进行比较判断矩阵的构建和计算,定量确定了各指标变量在牧草抗旱性综合评价体系中的权重及重要程度次序。     

节水农业文献数据库分类检索系统的建立──节水农业文献资源开发利用研究Ⅱ

遵照分类检索“以类索文，触类全貌，类目编排，严谨科学”的基本原则及本数据库独具专题综合性的特殊要求，以中国图管分类法为基础，优选有关１～４级类目、类号，显明简要的类目注释．并制定一套划分文献的规则，依类揭示本库所收录的节水农业文献信息，满足有关用户以类索文的需要。     

数据库技术探讨

如何充分利用有限的存储空间，提高嵌入式数据库的存储空间利用率一直是倍受关注的研究课题。介绍了嵌入式数据库中比较有代表意义的索引机制以及它们的优缺点，分析了B＋树索引机制的实现思想及其优缺点，实现了一种改进的索引机制B#树索引机制。B#树索引机制能在多消耗少量时间基础上，较大提高了嵌入式数据库的存储空间利用率。     

基于系统科学原理的知识组织理论研究

系统科学为知识组织的发展提供了理论基础和方法论基础,知识组织与系统科学是紧密相连的。笔者从系统科学原理角度对知识组织进行分析,以分类法和主题法为例,具体阐述了知识组织中所蕴含的整体性、层次性、相关性、动态性等系统科学原理。