高效液相色普法测定羟基喜树碱含量方法的研究(英文)

以高效液相色谱法作为羟基喜树碱含量检测方法,系统讨论了用超声法从喜树果实中提取羟基喜树碱用以制备分析样品的方法。采用HIQ sil C18柱(4.6×250 mm),流动相为乙腈:水(3:7,V:V),流速1 mL/min,检测波长266 nm。确定的最佳超声提取条件为:以60%乙醇为提取溶剂在60℃提取50 min。图3表1参10。     

羟基喜树碱制剂研究进展

综述了目前羟基喜树碱(HCPT)制剂类型研究的进展,包括脂质体、两亲性嵌段共聚物、脂肪乳、自微乳、纳米粒、胶束系统等多种剂型,这些剂型不仅提高了HCPT的溶解性,提供多种给药方式,而且增强了药物的稳定性,延长了HCPT在体内的作用时间,提高了药物的生物利用度,并且通过EPR效应不同程度地提高了HCPT对肿瘤细胞的被动靶向作用;并进一步提出叶酸可能会成为HCPT主动靶向作用的纳米制剂的新载体。     

Homogenate extraction technology of camptothecine and hydroxycamptothecin from Camptotheca acuminata leaves

Camptothecine (CPT) and hydroxycamptothecin (HCPT), two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C. acuminata leaves were determined as homogenate time at 8 min, ethanol concentration at 55% and the ratio of material to liquid at 1:15 (g:mL). By using the optimized parameters, we obtained 0.639‰ extraction rate for CPT and 0.437‰ for HCPT. The extraction yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic, reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting CPT and HCPT from C. acuminata leaves, with characteristics of less extraction time and high yield. Foundation project: This research was supported by National Key Technology R & D Program (grant no. 2006BAD18B04). Biography: SHI Wei-guo (1970–), male, Ph. D. candidate in Northeast Forestry University, Harbin 150040, P. R. China.)     

Homogenate extraction technology of camptothecine and hydroxycamptothecin from Camptotheca acuminata leaves

Camptothecine (CPT) and hydroxycamptothecin (HCPT), two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C. acuminata leaves were determined as homogenate time at 8 min, ethanol concentration at 55% and the ratio of material to liquid at 1:15 (g:mL). By using the optimized parameters, we obtained 0.639‰ extraction rate for CPT and 0.437‰ for HCPT. The extraction yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic, reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting CPT and HCPT from C. acuminata leaves, with characteristics of less extraction time and high yield.     

喜树水培体系中喜树碱、羟基喜树碱及其他组分的初步鉴定

为初步建立喜树水培体系并确定从其根系分泌物中提取喜树碱和羟基喜树碱的可行性,以喜树为研究对象,采集喜树幼苗,进行无土水培。利用高效液相色谱(HPLC)和气质联用(GC-MS)技术鉴定分析根系泌物中的喜树碱、羟基喜树碱以及其他成分。结果表明,喜树在水培体系中能够正常生长,根系发达,根系分泌物中存在一定含量的喜树碱、羟基喜树碱以及邻苯二甲酸二丁酯等化感物质。