白藜芦醇对骨髓损伤的修复作用

目的探讨白藜芦醇对辐射导致小鼠骨髓损伤的修复作用。方法 80只小鼠随机分为正常组、模型组、实验组和对照组,每组20只。除正常组外,其余各组以辐射造成小鼠骨髓型急性损伤模型;实验组和对照组分别腹腔注射白藜芦醇100mg/kg和甲基强的松龙100mg/kg,正常组和模型组则腹腔注射等量生理盐水,连续治疗7d,检测小鼠外周血白细胞、骨髓单个核细胞以及骨髓CD34＋细胞数。结果给药后实验组的白细胞计数、骨髓单个核细胞数和CD34＋细胞百分率与给药前比较差异均有统计学意义（P〈0.05）,且均高于同一时间点的模型组（P〈0.05）。实验组各时间点的白细胞数、骨髓单个核细胞数和CD34＋细胞百分率与对照组比较差异均无统计学意义（P〉0.05）。结论白藜芦醇可增加放射损伤小鼠白细胞、骨髓单个核细胞和CD34＋细胞的数目,加快骨髓造血功能的恢复。     

斑马鱼miR-30e对血细胞生成的作用机制研究

microRNA(简写为miRNA)是一类内源基因编码的单链RNA分子,参与转录后基因的表达调控,在血液生成过程中起着重要作用.为研究miR-30e对鱼类血液生成的作用机制,以斑马鱼Danio rerio作为模式生物,针对青藏高原裂腹鱼血液组织中高表达的miR-30e,利用生物信息软件预测斑马鱼miR-30e的靶基因,...     

造血和神经相关标志物在人胎盘的表达

为探讨人胎盘组织在造血中的作用以及研究造血和神经标志物在胎盘中的表达和在胚胎发育中的相关性，用免疫组织化学法对人胎盘组织和体外培养的胎盘组织中的贴壁细胞进行染色，观察其造血因子和神经标志物的表达。结果发现人7月龄胎盘组织血管内血细胞表达多种造血细胞因子，如SCF、VEGF、BMP-4和FGF-1和KDR，血管内皮细胞上也有表达；胎盘组织同时表达造血干细胞标记CD34、CD133和神经细胞标记物Nestin和MAP2等。而足月龄胎盘CD34、CD133、KDR和造血相关因子SCF、VEGF、BMP-4、FGF-1等弱表达，不表达Nestin和MAP2。两组胎盘切片都不表达GFAP和MBP。胎盘贴壁细胞（hPDACs）碱性磷酸酶、波形蛋白、CD133、Nestin和MAP2染色呈阳性，CD34、GFAP和MBP阴性表达。人胎盘组织和胎盘贴壁细胞表达多种造血相关因子和神经细胞标志物，提示胎盘具有造血功能，造血形成和神经发生之间可能存在基因表达叠加现象。     

阿胶泡腾颗粒对小鼠造血功能及抗疲劳作用的影响

研究阿胶泡腾颗粒对小鼠造血功能及抗疲劳作用的影响.通过制造盐酸苯肼型贫血小鼠模型,考察了阿胶泡腾颗粒对贫血小鼠红细胞数量及血红蛋白含量的影响;通过负重游泳试验、肝指数及肝糖原含量的测定,考察了阿胶泡腾颗粒对小鼠抗疲劳作用的影响.结果表明,阿胶泡腾颗粒能使盐酸苯肼型贫血小鼠的红细胞数量及血红蛋白含量明显回升,与空白对照组...     

“复方生血灵”生血效果及对骨髓细胞中RNA、DNA合成影响的研究

应用“复方生血灵”对实验动物的生血效果进行观察,并与已知补血药“益血生”作药效学比较,结果表明,连续应用“复方生血灵”对失血性贫血、缺铁性贫血等具有生血作用。对实验动物骨髓细胞中RNA的合成具有促进作用。     

Feline early life ocular disease

Objectives Histomorphologic changes in six globes from six cats, which experienced early life ocular disease of undetermined etiology, are described. Design A retrospective morphologic study of six eyes from six cats with early life ocular surface disease of unknown etiology, from 2002 to 2006 extracted from a pathology collection, which includes 2200 feline submissions. Procedure Sections of affected globes, stained with H&E were examined with a light microscope. Results The mean age of the affected cats, at the time of enucleation, was 7.5 months ranging from 7 weeks to 2 years. The cats were one male, one female, one male neutered, and one female spayed cat. For the remaining two cats the sex was not known. All cats were DSH. Significant histomorphologic findings included collapse of the globe in all cases and a broad corneal perforation with protrusion of the anterior uvea, which was epithelialized in all cases. Three cases revealed uveal hematopoiesis in the anterior and posterior uvea. All cases had recognizable corneal tissue at the limbus on both sides. Inflammation in all cases consisted of variable but generally mild uveitis and no eyes had endophthalmitis. Four of the globes had no recognizable lens tissue. Three of the cats had symblepharon formation described as part of the clinical condition. The other three cases had no mention of symblepharon. Conclusions These cases are considered to represent changes associated with early life corneal ablation of unknown etiology. Uveal prolapse, mild inflammation, and symblepharon are considered to be either secondary or caused by the same destructive primary event that affected the cornea. These cases are the first cases we are aware of with uveal extramedullary hematopoiesis in cats. Careful consideration of cell morphology is necessary to distinguish this condition from round cell neoplasms or inflammation.     

Targeted sequencing of candidate gene regions for myelofibrosis in dogs

BackgroundMyelofibrosis often lacks an identifiable cause in dogs. In humans, most primary myelofibrosis cases develop secondary to driver mutations in JAK2, CALR, or MPL.ObjectivesTo determine the prevalence of variants in JAK2, CALR, or MPL candidate regions in dogs with myelofibrosis and in healthy dogs.AnimalsTwenty‐six dogs with myelofibrosis that underwent bone marrow biopsy between 2010 and 2018 and 25 control dogs matched for age, sex, and breed.MethodsCross‐sectional study. Amplicon sequencing of JAK2 exons 12 and 14, CALR exon 9, and MPL exon 10 was performed on formalin‐fixed, decalcified, paraffin‐embedded bone marrow (myelofibrosis) or peripheral blood (control) DNA. Somatic variants were categorized as likely‐benign or possibly‐pathogenic based on predicted impact on protein function. Within the myelofibrosis group, hematologic variables and survival were compared by variant status (none, likely‐benign only, and ≥1 possibly‐pathogenic). The effect of age on variant count was analyzed using linear regression.ResultsEighteen of 26 (69%) myelofibrosis cases had somatic variants, including 9 classified as possibly‐pathogenic. No somatic variants were detected in controls. Within the myelofibrosis group, hematologic variables and survival did not differ by variant status. The number of somatic variants per myelofibrosis case increased with age (estimate, 0.69; SE, 0.29; P = .03).Conclusions and Clinical ImportanceSomatic variants might initiate or perpetuate myelofibrosis in dogs. Our findings suggest the occurrence of clonal hematopoiesis in dogs, with increasing incidence with age, as observed in humans.