Influence of Boar Seminal Plasma on the Distribution of Spermatozoa in the Genital Tract of Gilts

The main purpose of the present study was to investigate whether boar seminal plasma affects the transport of spermatozoa in the genital tract of oestrous pigs or not, with special reference to the sperm transport into the oviducts. Altogether 17 gilts were used in three experiments.Experiment I. In nine gilts one uterine horn was injected surgically with 10¹⁰ spermatozoa suspended in seminal plasma and the other uterine horn with 10¹⁰ spermatozoa suspended in TESNaK-glucose buffer solution. The sperm deposition was performed under general anaesthesia. The gilts were slaughtered 1–2 or 4–6 h after insemination. The genital tract was removed and the numbers of spermatozoa determined in oviducts and in uterine horns.Experiment II. The insemination doses were prepared exactly as in Experiment I. Approx. 24 h before insemination Polyvinylchloride cannulas were inserted into the uterine lumen of the horns, drawn via the midventral incision at linea alba subcutaneously to cutaneous incisions ventral to the vulva opening. One cannula was placed in each uterine horn. At standing heat the insemination doses were slowly injected through the cannulas. The gilts were slaughtered 1 h after insemination and the numbers of spermatozoa within the genital tract were counted.Experiment III. In three gilts under general anaesthesia the uterine horns were ligated 10 cm from the uterotubal junction. The semen doses (containing 2 × 10⁹ spermatozoa), prepared as in Experiment I, were deposited into the uterine horns anterior to the ligatures through a cannula. The gilts were slaughtered 1 h after insemination, and the numbers of spermatozoa within the oviducts and ligated part of the uterine horns were counted.In all three experiments more spermatozoa were, on average, recovered in the oviducts connected to uterine horns inseminated with spermatozoa suspended in seminal plasma. In Experiments I andII this was the case for 10 of 14 gilts and in Experiment III for all the three gilts. It is therefore suggested that boar seminal plasma pro¬motes sperm transport into the oviduct of oestrous pigs. The back¬ground mechanism for this is discussed.     

绿虎天牛属雌雄生殖器的比较研究

记述了绿虎天牛属Chlorophorus 8个种的雌雄生殖器，比较研究了该属雌雄生殖器的结构特点，结果表明，雌雄生殖器解剖性状在绿虎天牛属内是稳定的，在种间存在明显的差异，雌雄生殖器解剖特征可以作为该属分种的依据，是可靠的分类性状。     

Distribution of Live and Dead Spermatozoa in the Genital Tract of Gilts at Different Times After Insemination

Twenty-four gilts were inseminated pair-wise with live or dead spermatozoa from the same ejaculate. The insemination dose was 100 ml undiluted semen containing, on average, 19×10⁹ spermatozoa. The gilts were slaughtered 1, 2, 6 and 12 h after insemination. The numbers of spermatozoa were counted in the uterus, uterotubal junction and in four equally long segments of the oviduct, called I–IV, with a haemocytometer. IV was adjacent to the uterotubal junction. The numbers of spermatozoa recovered in the uterus diminished significantly during the first 12 h after insemination. From gilts inseminated with live spermatozoa more spermatozoa were recovered in the uterotubal junction than from gilts inseminated with dead spermatozoa. Two h after insemination spermatozoa were recovered in all oviducts. Significantly more live than dead spermatozoa were recovered in Segments III and IV of the oviduct, regardless of time. In gilts inseminated with live spermatozoa the sperm count in Segment I varied significantly with time, being hiigest 2 h after insemination. At 6 and 12 h there were no distinct differences in the distribution of live spermatozoa between the various oviduct segments. The numbers of spermatozoa recovered in the oviduct were at these times apparently related to the sperm depots in the uterotubal junction.     

通过测定生殖道黏液导电性确定小尾寒羊配种时机的观察研究

通过使用羊排卵测定仪准确掌握不同繁殖期小尾寒羊的排卵期,旨在有效提高母羊的受孕率。选择1.5~3.5周岁和4~6周岁小尾寒羊空怀期繁殖母羊各30只,利用羊排卵测定仪测定发情期内母羊生殖道黏液电阻值,研究其变化规律,发现1.5~3.5周岁母羊组发情持续时间较短,电阻值下降和上升均较快;而4~6周岁母羊组发情持续时间较长,电阻值下降较快但其上升曲线较为曲折。比较处于不同阴道黏液电阻值范围的母羊人工授精后的受孕率发现,4~6周岁的羊群组比1.5~3.5周岁的羊群组可受孕范围宽,但1.5~3.5周岁的羊群组在可受孕电阻值范围内受孕率明显高于4~6周岁的羊群组。该研究结果提示,不同年龄小尾寒羊母羊阴道黏液电阻值变化幅度不同,发情周期内电阻值下降到最低点后再上升时期为小尾寒羊的排卵期,适宜配种,该时期为母羊配种受孕率高。     

精子抗原基因在猪生殖道及成熟精子中的表达特性

【目的】揭示若干精子抗原基因(SPAG1、SPAG5、SPAG6、SPAG11C和SPAG11E)在梅山公母猪生殖道及成熟精子中的mRNA时空表达规律。【方法】利用RT-PCR技术分析各基因在生殖道和精子中组织表达谱,利用半定量RT-PCR分析其在初生至150d梅山公猪睾丸中的发育性表达特性。【结果】5个基因在梅山公母猪生殖道组织中呈现不同的表达特性。其中SPAG1在睾丸中表达丰度最高,在精囊腺、前列腺、附睾体、子宫角和输卵管呈现中等丰度表达,而在附睾尾、子宫颈和卵巢的表达信号较弱。SPAG5在睾丸中表达丰度最高,在前列腺、附睾体和子宫颈中表达信号较弱。而SPAG6在睾丸中高丰度表达,在输卵管中呈现中等丰度表达,在子宫角的表达信号很弱。SPAG11C在附睾体和睾丸中高水平表达,而SPAG11E则在附睾体和附睾尾中低水平表达。在成熟精子中只有SPAG11E有mRNA表达。SPAG1、SPAG5、SPAG6和SPAG11C基因在公猪睾丸中发育性表达分析表明,总体上所检测的4个精子抗原基因的表达水平都随着日龄增加而提高,但存在一些差异。其中SPAG1和SPAG11C呈现相似的表达规律,在初生和30日龄时表达相对较低,但在60、90、150日龄都有显著提高(P0.05)。而SPAG5在初生和30日龄时表达也相对较低,在60和90日龄时均有显著提高(P0.05),但150日龄时略有下降,但差异不显著。SPAG6在初生和30日龄时表达水平很低,至60日龄有显著提高(P0.05),90日龄时维持这一水平,而到150日龄时则有显著下降(P0.05)。【结论】SPAG1、SPAG5、SPAG6、SPAG11C和SPAG11E在梅山公母猪生殖道组织广泛表达,在睾丸组织中其mRNA表达水平变化与性发育相一致,SPAG11E在精子中存在低丰度mRNA表达。     

小地老虎的真核和无核精子在生殖道中的后期变化

通过电镜和光镜观察,追踪了小地老虎的真核和无核精子(束)在雄蛾和雌蛾的生殖道中发生的一系列后期变化.结果表明:真核精子在雄蛾贮精囊和复射精管中仍以精子束形式存在,但片层外长物全部消失;在雌蛾的受精囊中,其本体结构则从外套中脱壳“孵化”出来。无核精子在贮精囊中形成外套,在受精副囊中的无核精子绝大多数被分解.作者推论:大量的无核精子伴随着真核精子,对于真核精子的移动、营养、存活乃至最终的受精作用,都起着至关重要的作用。     

Vaginal myxoma in a Kathiawari horse

This case report describes a rare case of vaginal myxoma in a Kathiawari mare. The tumour, located on the left lateral vaginal wall, measured approximately 20 cm in diameter and resulted in dysuria, inappetance and loss of condition of the animal. Surgical enucleation of the tumour via a vaginal approach resulted in uneventful recovery of the horse. Based on the gross and histopathological observations, the tumour was diagnosed as a myxoma.     

健康奶牛生殖道乳酸茵的分离鉴定及生物学特性

通过MRS琼脂培养基厌氧培养技术,从产后7 d~60 d健康母牛子宫分泌物中分离得到7株乳酸菌,经生化试验鉴定2株为短乳杆菌,3株为海氏肠球菌,2株棉子糖肠球菌。生长曲线及产酸性能测定结果显示,所分离到的乳酸菌在培养2 h~4 h进入对数生长期,6 h~16 h进入生长稳定期;乳酸菌培养液在24 h内的pH由5.96~6.24下降为3.71~4.10。试验结果表明,所分离到的乳酸菌生长性能好、菌体密度高、产酸性能强,是一种有潜质的有益菌菌株。     

神经生长因子NGF及其受体TrkA在雌性水牛生殖器官的表达定位

为研究神经生长因子NGF及其受体TrkA在广西雌性水牛生殖器官中表达定位情况,运用免疫组织化学ABC法对处于发情周期不同阶段(卵泡期、黄体期)成年水牛的卵巢、子宫、输卵管NGF、TrkA分别进行染色定位.结果表明:NGF/TrkA阳性细胞在卵巢主要见于卵泡内膜细胞、颗粒细胞及黄体细胞;在子宫主要见于子宫内膜上皮细胞、腺...