沼液浸种增强早稻秧苗耐冷效应─—叶肉细胞超微结构动态观察

经沼液浸种的早稻秧苗比对照组秧苗，具有较强的耐冷性，通过电镜对叶肉细胞超微结构动态观察证实，在低温（５°／５℃）下，叶绿体结构和细胞核结构，随着冷害天数的延长，伤害在不断加重，叶绿体结构会出现空洞，基质片断裂，直至崩溃。细胞核染色体凝集，核膜破裂，核内容物丧失等现象出现。而经沼液浸种的秧苗伤害程度有较明显的减轻。     

菌株Bacillus sp.CLb芽孢漆酶对染料、模拟染料废水脱色的研究

[目的]研究芽孢杆菌菌株CLb的芽孢漆酶在介体的互作下对不同类型的染料及模拟染料废水的脱色效果。[方法]制备芽孢粗酶液,在介体的互作下,测定具有漆酶活性的粗酶液对活性亮蓝、活性黑、靛红和结晶紫的脱色效果,筛选出对染料脱色有促进作用的漆酶介体,并且研究芽孢漆酶在不同pH条件下对染料和模拟染料废水脱色的影响。[结果]菌株CLb的芽孢漆酶在无介体参与下只能使活性亮蓝和结晶紫脱色。在介体乙酰丁香酮（ACE）的作用下,芽孢漆酶对4种染料的脱色率均超过70%。在pH 7.0条件下,芽孢漆酶浓度为44.74 U/L和介体ACE浓度为1 mmol/L时对模拟染料废水的脱色效果最好,6 h脱色率超过80%。在介体乙酰丁香酮存在,pH 7.0的条件下,菌株CLb的芽孢漆酶对合成染料脱色效率高于pH 9.0时的脱色率。[结论]在介体乙酰丁香酮的存在下,芽孢漆酶对染料和模拟染料废水均具有较好的脱色效果。     

Nitrous oxide emissions from dairy farm effluent applied to a New Zealand pasture soil

A field experiment on permanent ryegrass–white clover pasture at AgResearch's Ruakura dairy farm near Hamilton, New Zealand quantified nitrous oxide (N₂O) emissions from different types of dairy effluent applied to soil at three seasons and evaluated the potential of dicyandiamide (DCD) (a nitrification inhibitor) to decrease gaseous N₂O emissions. Fresh or stored manure and farm dairy effluent (FDE; from dairy shed washings), with or without DCD (10 kg/ha), were applied at approximately 100 kg N/ha to plots on a well‐drained soil on volcanic parent material. A field chamber technique was used to measure N₂O emissions. Application of manure or FDE, both in fresh and stored forms, to pasture generally increased N₂O emissions. Overall N₂O emission factors (EF) varied between 0.01% and 1.87%, depending on application season and effluent type. EFs in spring and autumn were greater than those in summer (P < 0.05). Among the effluents, N₂O EFs were largest from fresh FDE (1.65%) during the spring measurement period, stored manure (1.87%) during the autumn and stored FDE (0.25%) during the summer. DCD was effective in decreasing N₂O EFs from fresh FDE, fresh manure, stored FDE and stored manure by 40–80%, 69–76%, 24–84% and 60–70%, respectively. DCD reduced N₂O emissions during the spring and autumn seasons more effectively than in the summer season.     

高氨氮厌氧消化液后处理技术研究

采用SBR-絮凝沉淀-Fenton氧化-活性炭吸附组合工艺对高氨氮厌氧消化液的后处理进行实验研究.结果表明,当进水COD浓度为4516 mg·L^-1时,SBR出水COD可降为1752 mg·L^-1,去除率达到61.2%;絮凝沉淀出水COD浓度为1189 mg·L^-1,去除率为32.1%;Fenton氧化和活性炭吸附出水COD浓度分别为452和216 mg·L^-1,去除率分别为61.7%和52.7%.当进水NH3-N浓度2162 mg·L^-1时,SBR出水NH3-N浓度降至13.8 mg·L^-1,去除率为99.4%,NH3-N主要在该段去除.整个组合工艺对COD和NH3-N的去除率分别达到95.2%和99.9%,达到了很好的有机物和氨氮去除效果,在技术上是可行的.     

碱法草浆造纸蒸煮黑液厌氧生物降解及其主要微生物类群研究

采用中温(35±1℃)UASB AF相结合的反应器处理碱法草浆造纸蒸煮黑液,设计了两种试验方案。试验方案Ⅰ-碱法草浆黑液酸化后进行厌氧发酵,Ⅰ号反应器内装陶粒。试验方案Ⅱ-黑液用化学法(HCl)去除木质素后进行厌氧发酵,Ⅱ号反应器内装软填料。进行HRT、有机负荷和有机污染物(COD_(cr)、BOD_5)去除效率、沼气产气率相关性试验。在厌氧发酵稳态运行条件下,采用Hungate厌氧操作技术,MPN计数法对UASB AF反应器内厌氧污泥中的主要微生物类群区系组成进行了生态分析研究。     

Utilization of biochar impregnated with anaerobically digested slurry as slow‐release fertilizer

We examined the possibility of an environment‐friendly slow‐release fertilizer (SRF) made of biochar impregnated by anaerobically digested slurry. The biochar materials were produced from three types of feedstocks (orange peel, residual wood, water‐treatment sludge) at different temperatures of 300°C, 500°C, and 700°C via pyrolysis. The release behaviors of the water‐soluble K⁺, Ca²⁺, and Mg²⁺ were similar for all impregnated biochars and the commercial SRF used. The water‐retention capacity was greatly improved by mixing the biochar‐SRF with the soil. The yield of lettuce was lower for the biochar‐SRF applications of 3.7 to 34.2 t ha^–1 than for the commercial SRF application of 51.4 t ha^–1. This might be due to excessive increase of soil pH for the biochar‐SRF application. Based on these results, the authors concluded that the biochar impregnated with nutrients could become an effective slow‐release K⁺ fertilizer.     

再生水加氯对滴灌系统堵塞及番茄产量与氮素吸收的影响

加氯处理是防止再生水滴灌系统生物和化学堵塞的常用方法,但有关加氯处理对作物生长影响的研究尚不充分。通过在日光温室内的田间试验,研究了加氯浓度和加氯频率对再生水滴灌灌水器堵塞特性、番茄产量和品质的影响;建立了土壤硝态氮含量随土壤电导率、含水率和温度变化的多元回归模型,分析了加氯处理对根区土壤硝态氮变化动态和氮素吸收的影响。结果表明,加氯处理能够有效防止再生水滴灌引起的灌水器流量降低;再生水滴灌增加了硝态氮在土壤表层（15 cm深度）的累积,促进了作物对氮素的吸收;加氯处理使植株吸氮量明显降低,加剧了硝态氮在土壤表层的累积,累积量随着加氯浓度和加氯频率的增大而增大;再生水滴灌的番茄产量略高于地下水滴灌,而再生水加氯处理会使产量有所降低;再生水滴灌使番茄口感指标（可溶性糖和水溶性总酸）显著提高、营养指标（Vc含量和可溶性固形物）显著降低,加氯处理能够有效缓解营养指标的降低趋势。采用浓度低于50 mg/L、频率低于两周1次的加氯处理对作物的氮素吸收有一定的抑制作用,但不会对作物生长造成明显不利影响。     

Mineral content of three olive cultivars irrigated with treated industrial wastewater

The reuse of saline treated industrial wastewater generated by textile firms mixed with municipal domestic effluent for irrigation was used to asses its effect on the mineral content of three olive (Olea europaea L.) cultivars under greenhouse and field conditions during two complete vegetative cycles. Chemical analysis of the treated wastewater indicated that the element concentrations fall within the permissible range of irrigation water used for plants. However, little impermissible accumulation of Na and Mg higher than the recommended maximum concentration was observed. Irrigation water with six electrical conductivities (EC = 0.78, 1.0, 2.0, 3.0, 4.0 and 5.0 dS m⁻¹ in treatments T₀, T₁, T₂, T₃, T₄, T₅, respectively) were compared in the greenhouse experiment. The olive trees in the field experiment were trickle irrigated with potable water and treated wastewater (average EC = 4.2 dS m⁻¹). The results of the greenhouse experiment showed that leaf N, Cu, Mn, Fe, Pb, and Na contents increased with increasing salinity of the treated wastewater. This increase was accompanied with a decrease in K and Mg contents. Leaf Ca and Cl concentrations were not considerably affected. Ion analysis in roots indicated that the contents of P, Na, Cl, Mn, and Pb increased while K decreased as treated wastewater salinity increased. Consequently, in most cases T₄ and T₅ gave a highly significant increase or decrease in accumulation of the previously mentioned minerals. A considerable variation in the studied cultivars was noticed. ‘Nabali’ was considered the most tolerant cultivar for the high salinity levels of the treated wastewater; its transporting selectivity of Na from root to leaf was higher and more Na was retained in the roots. Tissue analysis of leaves indicated that the element concentrations were within the adequate levels except those of Fe in ‘Nabali’ and ‘Manzanillo’, Na in ‘Improved Nabali’ and Cu in ‘Nabali’ and ‘Manzanillo’. In view of these findings, the negligible accumulation of minerals in leaves and roots indicated that this kind of textile effluent can be used as a valid alternative for irrigation of olive orchards with continuous monitoring of mineral levels.     

两种不同布水方式对生物砂滤池去除厌氧消化液氨氮的影响

在实验室条件下,采用试验生物砂滤柱模拟生物砂滤池系统,研究了雾化和滴渗布水方式对生物砂滤池去除猪场厌氧消化液氨氮的影响,结果表明:在进水水力负荷为0.057 m3.m-2d-1,氨氮平均浓度分别为293 mg.L-1,513 mg.L-1,553 mg.L-1时,雾化布水生物砂滤池对氨氮的平均去除率分别为98.8%,87.4%,75.1%,出水氨氮平均浓度分别为3.61 mg.L-1,64.7 mg.L-1,138 mg.L-1,平均去除负荷分别为16.4 g.m-2d-1,25.4g.m-2d-1,23.5 g.m-2d-1;滴渗布水生物砂滤池对氨氮的平均去除率分别为77.4%,71.4%,59.3%,出水氨氮平均浓度分别为66.3 mg.L-1,147 mg.L-1,225 mg.L-1,平均去除负荷分别为12.9 g.m-2d-1,20.7 g.m-2d-1,18.6 g.m-2d-1;雾化布水可以提高生物砂滤池氨氮的去除率、去除负荷,降低出水氨氮浓度;滴渗布水在进水氨氮平均浓度293 mg.L-1,雾化布水进水氨氮平均浓度293 mg.L-1,513 mg.L-1的条件下,出水氨氮平均浓度可达到《畜禽...     

三种消解方法测定天然橡胶镁含量的研究

为研究更适合于天然橡胶中镁含量检测的方法,采用干灰化法、微波消解法和石墨消解法三种消解方法研究不同消解条件对天然橡胶中镁含量检测的影响。结果表明,三种消解方法对天然橡胶镁含量的测定均可以满足要求。干灰化法对实验结果影响较小,对低镁含量的样品应适当增加样品量;微波消解法操作简便且检测耗时较短;石墨消解法操作简单,工作效率较高,适用于批量样品分析,设备以及耗材成本与前两种方法相比具有较大优势,为未来我国制定天然橡胶中镁含量的检测标准提供了理论依据。