绿色富硒生物猪饲料中AFB1达标生产工艺优化研究

饲料中黄曲霉毒素(AFB₁)容易超标,检出率达80%~100%,毒性大,具强致癌性,可抑制生猪免疫机能,降低动物生产性能,引起动物继发感染,还会在动物产品中残留而威胁人类健康,给生猪养殖带来经济损失,降低猪肉食品安全性。本文通过使用先进固体发酵系统设备和益生菌发酵技术,采用单因素试验和响应面中试优化,获得发酵降解猪饲料AFB₁的最佳工艺参数为硒浓度0.3 mg/kg,发酵时间12 h,量子波强度30 Hz,益生菌菌种组合CGMCC NO.17328混合CGMCC NO.15611。该工艺将猪饲料AFB₁量从63.41μg/kg降解到2.98μg/kg,降解率达到95.30%,AFB₁含量达到国家饲料安全标准。生产工艺适合养猪场低成本快速生产AFB₁达标猪饲料。     

不利气象条件对果业生产的影响及果园管理建议

针对近年来不利气象条件频发情况,分析了不利气象条件对果业生产的影响,提出减轻不利气象条件对果树影响的建议和不利气象发生后果园的管理要点。     

黄河中游水土保持措施保存率分析及措施状况评述──以黄河河龙区间南片为例

本文以土地详查、淤地坝普查成果为基本依据，结合典型调查，研究分析了河龙区间南片水土保持各项措施的保存率，认为在空间尺度上，分行政区给出的保存率较为真实的反映了社会环境、自然环境因素的相对作用程度及其主导关系，且有实际应用意义，据此文中进一步核实评述了该区水保措施的保存面积、措施结构及质量等。该区水保措施的状况在黄河中游干旱半干旱地区具有广泛的代表性，分析结果可作为水沙分析、水保规划、土地资源与环境评价等工作的重要参考依据。     

马铃薯品种抗软腐病测定条件的研究

用全薯块吸头刺伤接种法测定时,较理想的测定条件为:每接种点接种0.1ml浓度为10~2～10~5CFU/ml菌悬液,随后在21±5℃～31±5℃(依所用菌株而定)下保持2～3天,以接种点腐烂斑的最大直径作记载标准。用新鲜薯片法测定时,除接种浓度为10~2～10~3CFU/ml,保持时间为1～2天及以侵染限值作评价标准外,其余条件和全薯块吸头刺伤接种法相同。当用皮孔浸渍法接种时,薯块在10_6CFU/ml菌悬液中浸5分钟,然后使薯块表面保持连续水膜,3～5天后测量薯块表面腐烂面积。上述3种方法都必须有20个以上重复,并使用大小一致、无伤无病的成熟薯块。     

某类高阶非线性偏微分方程组弱解的正则性

本文研究一类2k阶非线性偏微分方程组之解的正则性,没有假定通常的椭圆性条件而只假定所谓"无穷远处"的椭圆性条件,证明了解的k-1阶导数为李普希兹连续的.     

Enantioselective degradation kinetics of metalaxyl in rabbits

Metalaxyl [methyl-N-(2′-methoxyacetyl)-N-(2,6-dimethylphenyl)-d,l- alaninate] is a potent phenylamide fungicide. The (−)-(R)-isomer accounts for most of the fungicidal activity. A possible stereo and/or enantioselective kinetics of metalaxyl in rabbits was investigated by intravenous injection. The concentrations of (−)-(R)- and (+)-(S)-metalaxyl in plasma, liver, and kidney tissue were determined by HPLC with a cellulose-Tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase and gas chromatography-mass spectroscopy. After intravenous administration of racemic metalaxyl (40 mg/kg), the (+)-(S)-enantiomer levels in plasma, liver, and kidney decreased more rapidly than the (−)-(R)-isomer. The area ratio of the (−)-(R)-/(+)-(S)-enantiomer under the concentration-time curve (AUC_0 → ∞) in plasma after drug application was 1.62. The total plasma clearance value of the (+)-(S)-enantiomer was 1.53 and higher than that of the (−)-(R)-enantiomer. The [R]/[S] ratio in plasma was >1 for standard rac-metalaxyl at each time point. The other pharmacokinetic parameters of the enantiomers were also different. The results indicate substantial stereoselectivity in the degradation of metalaxyl enantiomers in rabbits.     

反刍动物过瘤胃淀粉研究进展

过瘤胃淀粉是高产反刍动物葡萄糖的重要来源之一。本文阐述了过瘤胃淀粉在反刍动物营养中的作用,以及提高饲料过瘤胃淀粉比例的处理方法,并根据近年来的应用研究状况,提出了过瘤胃淀粉在应用中存在的问题及今后的研究方向。     

Influence of the physical and chemical properties of soil on the retention process of isoxaflutole and its two main derivatives

Isoxaflutole is a new pre‐emergence herbicide for use in maize and sugarcane. Its two main derivatives are a diketonitrile derivative, the 2‐cyano‐3‐cyclopropyl‐1‐(2‐methanesulfonyl‐4‐trifluoromethylphenyl)propan‐1,3‐dione, named DKN, and a benzoic acid derivative, the 2‐methanesulfonyl‐4‐trifluoromethylbenzoic acid, named BA. The adsorption/desorption processes have never been studied for isoxaflutole (IFT) at high concentrations nor for BA, and the present work aimed at completing the knowledge of the behaviour of these three molecules in conditions close to those encountered in the context of agricultural use. The adsorption/desorption study was conducted on seven soils of different physical and chemical properties, using the batch equilibrium technique. During the experiments, IFT was chemically converted into DKN in a continuous manner. This reaction appeared to be dependent on the pH of the soil and was taken into account in the calculations of the adsorbed and desorbed amounts. The adsorption isotherms obtained were predominantly C‐shaped for IFT and DKN and S‐shaped for BA, but some differences appeared on a few soils. They fitted well the Freundlich equation, and the values of the Freundlich coefficient K_fa showed that, whatever the soil, IFT was more adsorbed than its two derivatives. The main parameter influencing the adsorption of IFT appeared to be the organic matter content, whereas this effect was not evident for DKN and BA. No correlation was found between the extent of adsorption and either clay content or pH of the soil, for the three molecules.     

Effect of air temperature, rain and drought on hot water weed control

The influence of rain and drought before, and air temperature during, weed control with hot water was studied in laboratory experiments on the test weed Sinapis alba (white mustard). The plants were grown in a greenhouse and treated outdoors. There was no difference in weed control effect when S. alba plants at the four‐leaf stage were treated at the air temperatures 7°C and 18^°C. The effective energy dose for a 90% fresh weight reduction was 465 kJ m^?2 for both air temperatures. Weed control of S. alba at the four‐ to six‐leaf stage in rainfall above the rainwater run‐off level increased the required effective energy dose by 20% (i.e. 120 kJ m^?2) compared with dry plants. A short period of drought just before treatment on S. alba at the two‐ to four‐leaf stage increased the plant fresh weight reduction, which was 22% at low energy dose (190 kJ m^?2) and 44% at high energy dose (360 kJ m^?2). Hot water weed control should thus be carried out when the plants are drought stressed and avoided when the plants are wet. The air temperature seems to be of little importance in the range 7–18^°C.