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为明确北京地区南瓜病毒病种类及其主要侵染病原,2016~2017年在北京周边采集疑似感染病毒的南瓜病样84份,并根据南瓜上的6种病毒特异性引物对其进行反转录PCR(RT-PCR)检测。结果表明:共有79份南瓜病样检测显示阳性,其中黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为52.38%;其次是小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)和西瓜花叶病毒(Watermelon mosaic virus,WMV),检出率分别为44.01%、14.29%,其他病毒暂未检出。此外,16.67%的样品受2种病毒复合侵染,CMV和ZYMV复合侵染占7.14%,ZYMV和WMV复合侵染占9.52%。北京地区南瓜上优势病毒种类为CMV,且存在病毒复合侵染现象。 相似文献
3.
A disease causing the decline of papaya (Carica papaya) plants was noticed in the Jordan Valley in 1982. The disease caused severe yellowing of the upper leaves and dieback of the apex. The disease was not transmissible mechanically and continued efforts to associate viruses and viroids have so far been unsuccessful. Epidemiological observations suggested that the disease is airborne and probably caused by a mollicute. In order to test this hypothesis, plots were covered by an insect-proof net and with white nets providing 15, 30 and 50% shade. The 30%, 50% and insect-proof nettings provided complete protection, and the 15% netting reduced disease incidence to <2%, compared with>37% in the uncovered control plot. Spraying plants at weekly intervals with a commercial whitewash solution was also found to be effective in reducing disease incidence. The advantages of using a range of netting field treatments as a simple means for obtaining information on the epidemiological nature of a new disease are described. 相似文献
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对50例冠心病和50例随机做平板运动试验(TET)者作频域心电图(FCG)对比分析探讨FCG对冠心病的诊断价值。结果表明:单纯根据FCG异常诊断冠心病有较高假阳性(65.22%)。如将FCG的阳性指标作综合判断,虽然敏感性降低(88.00%降至70.00%)。假阳性都明显降低(65.22%降至26.00%)。认为TET的特异性较FCG高。若两者均为阴性,则冠心病可能性很小。 相似文献
6.
Melissa trogdon Hines DVM PhD Jerry R. Heidel DVM PhD David D. Barbee DVM MS 《Veterinary radiology & ultrasound》1993,34(1):47-51
A 2.5 year old Appaloosa stallion with progressive weight loss and a heart murmur was diagnosed as having vegetative endocarditis of the right atrioventricular valves with a mass in the right atrium. The ultrasonographic appearance of the lesion was characterized by extreme reverberation. On postmortem examination, a 9 cm diameter thrombus was found within the right atrium and auricle, occupying approximately 80% of the chamber volume. Additionally, a lenticular abscess extended throughout the parietal cusp of the right atrioventricular valve. Histologic examination revealed that the lesions were septic with numerous gram positive cocci in short chains, suggestive of Streptococcus equi , and gram negative rods. 相似文献
7.
介绍了山地橄榄2种病害:叶斑病、炭疽病;5种虫害:橄榄星室木虱、黑刺粉虱、恶性叶甲、橄榄枯叶蛾、橄榄野螟的主要特征和危害症状,并提出了防治措施。 相似文献
8.
陈耀星 《中国农业大学学报》1991,(3):118-121
本研究用 HRP 逆行追踪法对北京鸭心脏各区的迷走节前神经元胞体进行定位。标记细胞呈双侧性,集中在疑核和迷走背核,以疑核占优势(占68.4%)。在疑核,标记细胞多位于闩的嘴侧(峰值在+1000~+1800 μm水平),为较大型(φ20~30 μm)、多突起的星形细胞。在迷走背核,标记细胞呈梭形(φ18~20μm),大部分位于闩嘴懊I平面(+100~+1800μm)的腹侧部。左心室主要接受疑核支配。发自迷走背核的心迷走神经节前纤维主要分布在冠状沟。 相似文献
9.
D.H. Duvivier D. Votion S. Vandenput P. Lekeux 《Veterinary journal (London, England : 1997)》1997,154(3):189-202
Inhalation therapy plays an increasing role in the management of equine respiratory disorders. Thisalternative to systematic treatment permits a high concentration of medication to act locally while minimizing side effects and residues. In human medicine, literature in this field is prolific and continuously renewed, whereas in veterinary medicine, applications of aerosol therapy are less extensive. This review considers the principles of action of the different types of devices used for inhalation, i.e., nebulization, metered-dose inhalation and dry powder inhalation, describes the technical and practical requirements for their use in the equine species and considers the advantages and disadvantages of each inhalation device. The pharmacological agents currently administered to horses by inhalation are also discussed. Perspectives of aerosol therapy in the equine species, including aerosols already used in human medicine and their potential applications for horses are described. 相似文献
10.
K-S. Dai C-S. Liang Y. T. Ch'iu P-C. Yang I. C. Cheng 《Veterinary research communications》1995,19(2):115-125
The purpose of this study was to determine whether myocardial adenosine triphosphatase (ATPase) activities were reduced in pigs with naturally occurring hypertrophic cardiomyopathy (HCM). The selection of hearts for the HCM and the normal control groups depended on histological examination. Specific ATPase activity and 5-nucleotidase activity were measured in left ventricular myocardium obtained from HCM (n=7) and normal control (n=7) animals. The histological features of HCM included marked disorientation of muscle cells, thickening of the intramural coronary arterial wall with a narrowed lumen, endocardial fibrosis and myocardial fibrosis. The HCM group showed significant increases in both heart weight (32%) and heart weight to body weight ratio (46%). The total ATPase activity in crude homogenates from the HCM group was significantly decreased by 16%. Azide-sensitive ATPase (mitochondrial ATPase) activity, ouabain-sensitive ATPase (Na+,K+-ATPase) activity, basal Mg2+-ATPase activity and Ca2+-ATPase activity were all significantly decreased by 18%, 30%, 20% and 50%, respectively. In contrast, no significant decrease was found in the mean values for 5-nucleotidase activity. These results suggest that myocardial ATPase activities are suppressed in pigs with naturally occurring HCMAbbreviations ATP
adenosine triphosphate
- gww
grams wet weight
- HCM
hypertrophic cardiomyopathy
- Pi
inorganic phosphate 相似文献