Comparison of the microbial communities of alpacas and sheep fed diets with three different ratios of corn stalk to concentrate

The objective of this study was to investigate the characteristics of ruminal microbial communities of alpacas (Lama pacos) and sheep (Ovis aries) fed three diets with varying ratios of roughage (corn stalk) to concentrate, 3:7 (LS), 5:5 (MS) and 7:3 (HS). Six alpacas (one-year-old and weighing 29.5 ± 7.1 kg) and six sheep (one-year-old and weighing 27.9 ± 2.7 kg) were used in this study, in a replicated 3 × 3 Latin square experiment. Total protozoa concentration was determined under the microscope; total fungi and methanogens were assessed using quantitative polymerase chain reaction and expressed as a percentage of total bacterial 16S rRNA gene copies; bacterial communities were investigated by targeted 16S rRNA gene (V3–V4 region) sequencing. The percentage of fungi was significantly higher in alpacas than in sheep under the LS diet, while the concentration of protozoa was significantly lower in alpacas under HS, MS and LS diets. The alpha diversity including Shannon, Chao l and ACE indices of bacterial communities was higher in alpacas than in sheep, under the LS diet. A total of 299 genera belonging to 22 phyla were observed in the forestomach of alpaca and sheep, with Bacteroidetes and Firmicutes dominating both animal species. Phyla Armatimonadetes and Fusobacteria, as well as 64 genera, were detected only in alpacas, whereas phyla Acidobacteria and Nitrospira, as well as 44 genera, were found only in sheep. The abundance of cellulolytic bacteria, including Butyrivibrio and Pseudobutyrivibrio, was higher in alpacas than in sheep under all three diets. These differences in the forestomach microbial communities partly explained why alpacas displayed a higher poor-quality roughage digestibility, and a lower methane production. Results also revealed that the adverse effects of high-concentrate diets (70%) were lesser in alpacas than in sheep.     

The microbial diversity and structure in peatland forest in Indonesia

The microbial community structure and function under forest in tropical peatlands are poorly understood. In this study, we investigated the microbial community structure and diversity in natural peat swamp forest soil, disturbed peat soil and mineral soil in Central Kalimantan, Indonesia, using 454 pyrosequencing. The results showed that the natural peat soil had the greatest fungal species richness (Chao1), which was significantly (p < .05) larger than that in the other two soils. Community structure of both fungi and bacteria in natural peat soil differed significantly from that in the disturbed peat soil (p = .039 and p = .045, respectively). Ascomycota (40.5%) was the most abundant phylum across the three soils followed by Basidiomycota (18.8%), Zygomycota (<0.1%) and Glomeromycota (<0.1%). The linear discriminant analysis with effect size (LEfSe) showed that Ascomycota (p < .05) and genus Gliocephalotrichum (p < .05) dominated in natural peat soil. Functionally, pathotrophs were more abundant in disturbed peat soil (p < .05). Proteobacteria (43.8%) were the most abundant phylum followed by Acidobacteria (32.6%), Actinobacteria (9.8%), Planctomycetes (1.7%). Methylocystis, Telmatospirillum, Syntrophobacter, Sorangium and Opitutus were the more abundant genera in disturbed peat soil, whereas Nevskia and Schlesneria were more abundant in mineral soil and natural peat soil, respectively. The natural peat forest soil supported a more diverse microbiology; however, the land use of such a soil can change its microbial community structure. The results provide evidence that the disturbance of tropical peat land could lead to the introduction and spread of a large number of fungal diseases     

Can trail spatial attributes predict trail use level in urban forest park? An examination integrating GPS data and space syntax theory

Understanding users’ spatial distribution in forest park is crucial for providing visitors with quality recreation experiences and for park planning and management. Utilizing users’ spatial distribution data, this study aims at investigating associations between trail use level and trail spatial attributes, through examining two large urban forest parks (Gongqing forest park and Paotaiwan forest park) in Shanghai, China. Users’ spatial distribution was measured utilizing GPS trackers with the interval of 10 seconds. This study conceptualizes trail spatial attributes as trail metric attributes and trail configurational attributes. Trail metric attributes include trail mean distance to gates, length, width and level of enclosure, which are calculated based on park map and on-site observation. We computed trail configurational attributes utilizing space syntax theory, which comprise measures of global integration, control, and connectivity. Trail connection with features/facilities, visual connection with water and shading are included as covariate variables. In total, we obtained 134 valid samples in Gongqing forest park and 210 valid samples in Paotaiwan forest park for analysis. Multivariate regression analyses indicate that when involving covariate variables, consistently in both parks, a greater trail usage is significantly related to trail shorter mean distance to park gate, trail width wider than 3 meters, higher global integration and higher control values. Collectively, these four trail spatial attributes explained 31.7 % (p < 0.001) and 27.3 % (p < 0.001) of the variances in trail use level in Gongqing forest park and Paotaiwan forest park. These findings provide direct implications to park designers and managers for providing visitors with different desirable social conditions, and ultimately improve users’ experiences and satisfactions.     

社会林业与五指山的自然保护

五指山自然保护区是世界上现存地势最高的原始热带雨林之一，是我省目前面积最大的自然保护区，也是海南三大河流的发源地。但由于保护区横跨琼中、通什、保亭三县、市，紧邻保护区还生存着众多贫困的黎、苗族村民。因此，保护区与周边社区的联系是十分紧密的，社区的发展也直接影响到五指山热带天然林的保护。海南省林业局中德海南热带林保护和恢复项目在社会林业与五指山森林的保护上做出了有益的尝试，就在全省来说，也是首次对社会林业与森林保护上进行的探索。五指山地区社会林业的研究，对保护海南热带雨林也有着积极的意义。特对社会林业与五指山的森林保护进行了阐述、分析和设想。     

城镇化水平计算方法比较分析

城镇化是社会经济发展的必然趋势，与小城镇人口、经济实力、社会发展和环境保护等方面密切相关。城镇化判别指标归纳起来分两大类：一类是单一指标法或称主要指标法；另一类是多项指标法、复合指标法或称综合指标法。在常用的、单一指标法的城镇化水平计算方法中，城镇人口比重指标法和带眷系数法两种方法能够比较准确地反映小城镇的城镇化水平，尤其以城镇人口比重指标法计算结果最符合实际。     

关中地区几种苜蓿地上生物量初步研究

对5个苜蓿Medicago sativa品种的地上生物量形成规律进行了初步研究.结果表明:苜蓿生物量的增长速率不仅与本身生理特性有关,而且与环境密切相关;株高在分枝末期现蕾初期增长速度快.绝对生长速率与相对生长速率在分枝末期现蕾期都较大,此时的水分管理具有重要意义;不同苜蓿品种的群落结构大多为菱形;同化系统与非同化系统的比值随高度的增加而减少.     

不同能量水平对羊生产性能及激素水平的影响

选用山羊和绵羊各21只,研究能量水平对羊采食量(DMI)、日增重(ADG)、饲料转化率(FCR)、胰岛素、生长激素和孕酮浓度的影响。结果表明:绵羊的DMI显著大于山羊的(P<0．05),低能量水平下,绵羊的DMI显著大于山羊的(P<0．05)。绵羊的ADG显著高于山羊的(P<0．05),高能量水平下两种羊的ADG显著高于低能量水平的(P <0．05),FCR在绵羊与山羊之间、不同能量水平之间及互作之间都不显著(P>0．05)。试验前期山羊血清胰岛素水平显著大于绵羊的(P<0．05),在低能量水平下,山羊的血清胰岛素浓度显著高于绵羊的(P<0．05)。试验前期山羊的孕酮水平显著高于绵羊的(P<0．05),高能量水平下的孕酮极显著地高于中、低能量水平下的孕酮(P<0．01),中等能量水平下,山羊的孕酮浓度显著高于绵羊的(P<0．05)。     

施肥对红三叶群落营养元素含量和积累量的影响

本文研究了施肥对红三叶群落地上部和地下部 1 0种营养元素的影响 ,结果如下 :( 1 )从含量看 ,施氮肥后 ,地上部B明显降低 ,地下部B明显升高 ;施磷肥后 ,地上部N、B显著下降 ,地下部K、P、N上升幅度较大。 ( 2 )就积累量而言 ,施氮肥后 ,地上部大多数元素增高 ,地下部多数元素降低或无变化 ;施磷肥后 ,无论地上部还是地下部 ,大多数元素均有所增高     

蒙特卡罗模拟信号交叉口的服务水平

信号交叉口是城市路网的节点和交通关键，其服务水平反映了交叉口所提供的道路及控制条件满足交通顺畅、安全等需要的程度。本文提出一种基于蒙特卡罗模拟的信号交叉口运行状态描述和服务水平评价方法．为优化信号交叉口控制参数提供依据．     

Study on ambient noise in sawmill’s workshop

Ambient noise in 13 sawmill’s workshops was tested and analyzed to reveal its character and regularity. The situation of the ambient noise in sawmill’s workshops was evaluated