Characterization of bovine ileal epithelial cell line for lectin binding,susceptibility to enteric pathogens,and TLR mediated immune responses

In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.     

鸡源株与人源株H9N2流感病毒血凝素受体结合位点氨基酸比较与分析

H19N2病毒可以感染多种禽类和哺乳动物，包括人类。流感病毒血凝素受体结合位点的氨基酸可以影响受体结合特性。为了解鸡源株与人源株H19N2病毒受体结合部氨基酸的差异，作者对二者进行了比较与分析，结果表明，鸡源株与人源株血凝素受体结合部位氨基酸的第137、183、190、226位点存在差异，鸡源株在这些位点分别为K、N、AorVorT、LorQ而人源株则分别为R、H、E、L。虽然尚不清楚这些位点的改变对病毒与细胞亲和力及宿主范围的影响，但由于H9N2在我国养鸡业的普遍存在，加强H9N2病毒的分子流行病学监测有重要意义。     

棉花黄萎病菌毒素结合位点初探

以纯化的棉花黄萎病菌毒素(PLPC)免疫新西兰白兔制备了PLPC特异性抗血清。将PLPC(15μg·ml 1)用不同浓度的抗体(1～20μg·ml 1IgG)吸附后处理泗棉3号的切根苗,毒素所引起的症状都有不同程度的减轻。表明所制备的抗体在与毒素发生特异性免疫学反应的同时,可部分封闭毒素分子上与毒素受体结合的位点。利用竞争ELISA测定了泗棉3号幼苗子叶的质膜制剂与PLPC的结合活性。结果表明,质膜制剂与毒素结合后能部分阻断毒素与其抗体的免疫学反应,即质膜制剂中含有毒素的结合位点。分别用胰蛋白酶和煮沸处理质膜制剂后,质膜制剂对毒素与其抗体的反应的抑制作用消失,初步表明质膜制剂中与毒素结合的是蛋白质。     

农业土地规模经营的理论探讨

实现农业土地规模经营,有利于生产力有效运转并取得最佳的规模效益。其适度性受各种因素的影响,具有区域性和动态性。规模经营与劳动生产率有着密切的关系,应该在确保提高土地生产率的基础上来提高劳动生产和土地规模经营的效益。土地适当集中、土地经营主体的总人数尽量精简和劳动组合的优化是优化土地经营规模的三个相互制约的因素。要采取积极的措施逐步实现土地的规模经营。对现有土地经营形式要做具体分析,其调整应从有利于生产力发展出发,在尊重群众意愿的基础上稳步进行。     

以彝人古镇为例论我国新兴旅游形式的发展

旅游是社会生产力发展到一定阶段的产物,是人们在满足了基本生活需要的基础上逐渐所形成的更高层次的消费需求,随着经济的发展以及社会不断的进步,人们思维方式的不断转变对于旅游的要求也逐渐发生了转变。     

Adrenomedullin (AM) and adrenomedullin binding protein (AM-BP) in the bovine mammary gland and milk: Effects of stage of lactation and experimental intramammary E. coli infection

Adrenomedullin (AM) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. Because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of AM and its binding protein (AM-BP) might be affected by the stage of lactation and the udder health status. Milk and mammary biopsy samples were obtained from Holstein cows 21 days prior to and at various times after calving to represent the dry period and early and mid-stages of lactation. Additional cows received an intramammary challenge with Escherichia coli for immunohistochemical characterization of AM and AM-BP. Milk AM concentrations were relatively constant across the stages of lactation while AM-BP increased two-fold (P<0.04) between early and mid-lactation. Milk AM (P<0.04) and AM-BP (P<0.03) increased as somatic cell counts (SCCs) increased within a given stage of lactation. Tissue content of both (AM and AM-BP) were significantly affected by stage of lactation, lowest in the dry period and progressively increasing to peak at mid-lactation as well as increasing in association with higher levels of SCCs. Following E. coli challenge, AM increased in epithelial cells surrounding mammary alveoli presenting high levels of SCCs. The data suggest that AM and AM-BP are cooperatively regulated in the mammary gland during lactation; changes in localized tissue AM and AM-BP content reflect a dynamic regulation of these tissue factors in the bovine mammary gland consistent with their protective effects within inflamed tissue.     

Comparative pharmacokinetics of marbofloxacin in healthy and Mannheimia haemolytica infected calves

The pharmacokinetics of marbofloxacin were investigated in healthy (n=8) and Mannheimia haemolytica naturally infected (n=8) Simmental ruminant calves following intravenous (i.v.) and intramuscular (i.m.) administration of 2 mg kg(-1) body weight. The concentration of marbofloxacin in plasma was measured using high performance liquid chromatography with ultraviolet detection. Following i.v. administration of the drug, the elimination half-life (t(1/2 beta)) and mean residence time (MRT) were significantly longer in diseased calves (8.2h; 11.13 h) than in healthy ones (4.6 h; 6.1 h), respectively. The value of total body clearance (CL(B)) was larger in healthy calves (3 ml min(-1) kg(-1)) than in diseased ones (1.3 ml min(-1) kg(-1)). After single intramuscular (i.m.) administration of the drug, the elimination half-life, mean residence time (MRT) and maximum plasma concentration (C(max)) were higher in diseased calves (8.0, 12 h, 2.32 microg ml(-1)) than in healthy ones (4.7, 7.4 h, 1.4 microg ml(-1)), respectively. The plasma concentrations and AUC following administration of the drug by both routes were significantly higher in diseased calves than in healthy ones. Protein binding of Marbofloxacin was not significantly different in healthy and diseased calves. The mean value for MIC of marbofloxacin for M. haemolytica was 0.1+/-0.06 microg ml(-1). The C(max)/MIC and AUC(24)/MIC ratios were significantly higher in diseased calves (13.0-64.4 and 125-618 h) than in healthy calves (8-38.33 and 66.34-328 h). The obtained results for surrogate markers of antimicrobial activity (C(max)/MIC, AUC/MIC and T > or = MIC) indicate the excellent pharmacodynamic characteristics of the drug in diseased calves with M. haemolytica, which can be expected to optimize the clinical efficacy and minimize the development of resistance.     

Philosophy of Science and the Chinese Contemporary Architectural Forms

Philosophy of science led by Popper is one of the most important contemporary scientific and philosophic theories.In addition to the science,the philosophy of science impacted contemporary social science mostly,of which the false-feasibility,circumstantial logic,anti-historical determinism and anti-scientism influenced the orientation of contemporary architecture.Just with these factors of philosophy of science,this thesis reanalyzed and reviewed Chinese contemporary architectural forms,especially the tradition inheritance.     

雨养烟叶种植田无机氮淋溶特征

为了解烤烟种植下土壤氮淋溶与大田作物差异,评价烟田常规养分管理,探寻烟田无机氮淋溶的阻控策略。以贵州龙岗长期定位试验为平台,于2015—2017年开展常规管理下烟田氮素淋失及其影响因素研究。试验设5个处理:不施肥(CK)、化肥(NPK)、化肥+厩肥(NPK+M)、化肥+连作(NPK+L)、化肥+生物有机肥(NPK+BM)。结果表明,烟田全年无机氮淋溶量为3.62~6.08 kg/hm^2,氮肥净淋溶率为0.09%~3.29%。无机氮的淋溶损失主要发生在烤烟生长季,尤其是5—6月,其占总淋溶量的40.33%~65.86%。烟田淋溶液中氮素形态主要是有机态,无机氮的比例平均仅为29.83%,缓苗期和旺长期(5—6月)淋溶液中无机氮比例高于烤烟成熟期(7—8月),前者无机氮比例平均33.00%,后者其平均为26.67%。降雨是影响烟田淋溶损失的主要因素,无机氮淋溶量与月降雨量呈非线性相关。施用化肥导致无机氮淋溶显著升高,有机肥配施化肥降低了土壤溶液淋溶,降低了氮肥淋溶损失。与烤烟玉米轮作处理相比,烤烟连作处理显著降低了土壤水淋溶,使氮肥净淋溶率降低59.57%。综上,目前烤烟常规管理下,雨养农业区烟田无机氮淋溶强度不高,受降雨影响大,应注重有机无机配施降低无机氮淋溶,在养分管理中考虑如何降低有机氮淋溶,以提高氮素养分供应量。