全文获取类型
收费全文 | 215篇 |
免费 | 8篇 |
国内免费 | 20篇 |
专业分类
林业 | 2篇 |
农学 | 2篇 |
基础科学 | 2篇 |
2篇 | |
综合类 | 64篇 |
畜牧兽医 | 169篇 |
植物保护 | 2篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 5篇 |
2019年 | 12篇 |
2018年 | 6篇 |
2017年 | 13篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 8篇 |
2012年 | 9篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 1篇 |
2007年 | 8篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 13篇 |
2003年 | 14篇 |
2002年 | 9篇 |
2001年 | 8篇 |
2000年 | 7篇 |
1999年 | 13篇 |
1998年 | 14篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1995年 | 2篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 5篇 |
1990年 | 6篇 |
1989年 | 3篇 |
排序方式: 共有243条查询结果,搜索用时 15 毫秒
1.
Qi Wang Quanwei Zhang Ze Gan Haijiang Li Yang Yang Yong Zhang Xingxu Zhao 《Reproduction in domestic animals》2020,55(2):189-199
Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non-oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up-regulated and 21 were down-regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac-related pathways, such as ‘dilated cardiomyopathy’, ‘hypertrophic cardiomyopathy’, ‘cardiac muscle contraction’ and ‘adrenergic signalling in cardiomyopathy’. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus. 相似文献
2.
本试验旨在研究骆驼乳清蛋白(CWP)对热应激(HS)大鼠肝损伤、氧化应激及肝功能的保护作用。选取6周龄SD大鼠36只,适应性饲养2周后随机分为6组:正常对照组饲喂基础日粮;CWP对照组添加400 mg·kg-1的CWP;HS组除饲喂基础日粮外,每天进行2 h HS处理,连续8 d;3个CWP干预组分别于每次HS前灌服100、200和400 mg·kg-1的CWP。试验结束后,取大鼠肝组织,HE染色观察肝组织病理学变化,同时检测肝功能生物标志物、氧化应激标志物水平及抗氧化酶活性。结果表明:CWP降低了HS大鼠血清谷草转氨酶(AST)和谷丙转氨酶(ALT)活性,400 mg·kg-1的CWP干预效果最好(P<0.01);400 mg·kg-1CWP干预组有效降低了HS诱导的大鼠肝组织病理学改变; CWP降低了大鼠肝活性氧(ROS)及丙二醛(MDA)水平,增加了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及谷胱甘肽(GSH)水平,400 mg·kg-1的CWP干预效果最好(P<0.01)。结果提示,CWP干预能够以剂量依赖性方式降低大鼠肝氧化应激,增加抗氧化系统防御能力,从而缓解HS所致大鼠肝损伤。 相似文献
3.
ZHANG Meng-hua CHEN Gang-liang ZANG Chang-jiang DONG Jing HUANG Xi-xia LU Dong-lin 《中国畜牧兽医》2016,43(10):2628-2633
This study was aimed to systematically analyze the difference of camel milk physicochemical index of different seasons,stage of lactation,production sites and milk station.Collected 18 372 testing data about raw camel milk quality which were purchased by a company in 2015.The range of each index was determined by the four point test method,SAS 8.1 least squares analysis of variance were performed on the processed data.The results showed that different area,milk stations,stage of lactation and season effect of camel milk had extremely significant influence (P<0.01) on the relative density,fat,non fat milk solid,milk protein,lactose,freezing point and ash.Therefore,the reproduction and childbirth of Bactrian camel had strong periodicity and seasonality,lead to a larger difference in camel milk yield and physicochemical index of different seasons and months within a year,at the same time,camel milk producers could learn about the quality of camel milk situation of different production sites,milk station,stage of lactation and season using such analysis methods,the quality of camel milk product was determines by the physical and chemical indicators and health conditions of the raw camel milk. 相似文献
4.
5.
对5具双峰驼后肢骨骼进行了形态学观察,并与单峰驼、马、牛的后肢骨骼做了比较,发现双峰驼后肢骨骼的特征如下:髋结节薄而窄小,荐结节厚而宽大;坐骨结节粗大,坐骨弓呈深“V”字形;转子窝很深,约3.5cm,无第三转子;膝盖骨呈上下行的椭圆球形;胫骨很发达,其骨干的外侧部较内侧部锐利;腓骨退化,近端只残留一痕迹,远端退化成胫骨远端外侧部下伸的踝突,无骨干;跗骨有6块排成3列。后肢其余的脚骨(跖骨、近趾节骨、中趾节骨和籽骨)较前肢相应的脚骨细、长,亦无远籽骨。 相似文献
6.
在光镜及电镜下,双峰驼骨骼肌的肌外膜与肌束膜均较厚,其中除有不等的胶原纤维、弹性纤维、网状纤维、成纤维细胞及血管、神经外,还有丰富的脂肪细胞。在肌纤维外有薄层肌纤维膜,后者与肌内膜相连。骨骼肌肌纤维一般较粗,但其粗细差异很大(长约4~55mm,宽约9~125μm),呈长短不一的圆柱状、A带、I带相间的横纹明显。肌原纤维也较粗大(直径约7~14nm,长约15~2.5μm)。肌原纤维间的肌浆中响大量线粒体及糖原颗粒。线粒体体积大,嵴较长,呈典型的板层状。 相似文献
7.
8.
Nuh Kl Nihat Toplu Ahmet Aydoan Rahime Yaygngül ule Yurdagül
zsoy 《Veterinary ophthalmology》2010,13(Z1):100-102
A 15‐year‐old male dromedary camel with a history of chronic severe keratoconjunctivitis and corneal mass in the left eye of 6 months’ duration was referred to the Veterinary Medical Teaching Hospital at Adnan Menderes University. A superficial keratectomy was performed and biopsy material submitted for histopathology. The diagnosis was corneal papilloma. There has been no recurrence of the neoplasm to date (6 months, 1 year). Corneal papilloma has not been reported previously in camels and seems to be associated with papillomavirus. 相似文献
9.
Haematological estimations and serum biochemical analyses were made on 100 samples collected from clinically healthy Hijin racing camels (Camelus dromedarius) in Kuwait. The red blood cell counts, packed cell volume, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and total white blood cell counts were estimated. In the serum biochemical analyses, sodium, potassium, iron, calcium, phosphorus, magnesium, total bilirubin, blood urea nitrogen, creatinine, glucose, total protein and cholesterol concentrations were measured, as were the alanine aminotransferase, aspartate aminotransferase, -glutamyltransferase, creatine kinase and alkaline phosphatase activities. The results are discussed in relation to other findings reported in camels. 相似文献
10.
Gutierrez C Corbera JA Doreste F Padrón TR Morales M 《Veterinary research communications》2002,26(6):437-442
In 1998, two cases of silica urolithiasis occurred in castrated male dromedaries on an intensive camel farm in the Canary Islands. The immediate attributable cause was the ingestion of large amounts of silica in the feed, estimated as 84.44 g/day. An associated cause was the low level of salt in the diet. Daily ingestion of salt from feed and water was estimated to be 21.8 g (8.6 g of sodium). Seventy-six castrated males from the same farm were divided into four groups: group A received 30 g of salt daily; group B received 40 g; group C received 60 g; and group D received no added salt in the diet (control). The animals were maintained on these dietary regimes for 2 years. No animals from groups A, B or C suffered overt urinary retention. One animal from group D had an obstructive urinary retention 10 months after the study commenced. Thus, 52 g of salt daily appears to be sufficient to prevent urinary retention in dromedaries raised in a subtropical climate. 相似文献