Effects of music stimulus on behavior response,cortisol level,and horizontal immunity of growing pigs

An enriched environment is widely used to improve domestic animals’ welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs.     

遗传背景对水稻无融合生殖材料C1001双胚苗频率影响的研究

同核异质雄性不育材料与无融合生殖材料Ｃ１００１Ｂ杂交和回交表明，Ｃ１００１Ｂ的双胚苗，是受核基因控制的性状，双胚苗频率的表达，具明显的胞质效应和胞核效应，在胞质效应中，不育胞质与可质胞质的差异尤其显著，不育胞质对双胚苗的表现有明显的抑制作用，不同世代间胞质效应和胞核效应的表现也不一致。此外，环境条件对Ｃ１００１的双胚苗表现有一定的影响。     

Development of a semi-selective medium and an immunofluorescence colony-staining procedure for the detection of Clavibacter michiganensis subsp. sepedonicus in cattle manure slurry

Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 10⁸ colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×10³ and 2.3×10⁴ cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R ²=0.74).     

植物化感作用影响因素的再认识

土壤养分、酶、微生物、本底特性,根茬还田、水分、植物根系、种植体系和种植模式与化感作用的互作关系,表明这些因子可通过对土壤养分有效性、微生物种群结构、土壤酶活性和化感物质浓度的影响而对化感作用产生直接或间接影响,这些可为通过化感作用途径构建高效农田生态系统提供新的思路.     

陕西百年农业科技发展研究

笔者回顾了陕西百年来不同时期农业科技发展的历史背景与社会环境,分析了陕西农业科技发展的动因与本质、范围与对象、社会需求等内在属性特征;总结了陕西农业科技发展的主要成效,即构建了农业科研技术服务体系、培育推广了技术良种方法、发展了农业产业、形成了农业科技发展的政策人才环境、改变了农业传统观念与生产方式;归纳凝练出陕西农业科技发展的经验启示,即农业科技发展是解决农业诸多矛盾的必然要求、实现农业现代化的有效途径、构建农业安全的重要举措,同时农业科技发展必须以国家需要、人民需求为根本出发点。指出陕西农业科技发展中成果转化率不高、体制机制僵化、省级支持不够、基层人员结构不合理等问题,提出通过“科研面向需求”、设立省级基金、开展农技有偿服务、扩大农技部门人事权限、发挥各类市场主体作用来解决问题,推动全省农业科技持续稳步发展。     

《荀子》音乐教化思想实质及价值

荀子为儒学大师，主张性恶论，力推人文教化，尤其重音乐教化，试图借助音乐等艺术作品来打造充满人文气息的文化氛围，尊礼崇礼，使人的尊贵得以凸显，人禽有别。同时，他认为人人经过自身的修为都可以成为善人，君子与小人的区别在于自己的把控。这些观点对于推行人文教化大有启示：即要重视音乐等艺术作品及良好文化氛围对人的熏陶与“化”的作用，特别是他的重养人之情、养人之欲观点在当下对于制定规范措施有借鉴的价值。     

湘桂走廊白背飞虱早期虫源及灾变大气背景

为阐明湘桂走廊白背飞虱早期虫源及大气背景在其北迁过程中的影响,利用HYSPLIT软件模拟白背飞虱灾变性迁入高峰期、始见期后向轨迹,分别从个性及共性角度,分析湘桂走廊白背飞虱主要虫源地及其灾变大气背景。结果表明：（1）1991—2014年湘桂走廊白背飞虱的迁入始见期、首次迁入高峰期分别为0.9 d/10 a、1.6 d/10 a;（2）西南路径是湘桂走廊白背飞虱初始虫源迁入的主要路径,永福站、兴安站和全州站虫源西南路径占比分别为54%、50%、72%;（3）中南半岛白背飞虱借助微弱上升气流完成起飞,850 hPa高度上的强劲的西南气流、丰富的水汽条件有利于其北迁,降水是白背飞虱降落的重要大气动力机制。研究结果为白背飞虱的异地监测及科学防治提供科学依据。     

儒学审美意识与中国古典园林的交融

儒家思想及其审美意识对中国古典园林的产生和发展影响深远。笔者先从早期儒学与园林肇端的交融谈起,说明儒家主导思想及审美意识与中国古典园林的交融,主要体现在礼乐人格塑造、山水比德思想、隐逸出世思想等方面,挖掘中国造园思想的哲学、美学根源,及其对现代园林景观的启示。     

论西方音乐本质观的三种模式及其演变规律

在西方音乐美学史上，出现了三种音乐本质观的模式：即以柏拉图和亚里斯多德为代表的古典时期的模仿论，以黑格尔和李斯特为代表的浪漫时期的表现论和以汉斯立克等为代表的现代时期的形式论。每一种模式的出现都扎根于时代的思潮和哲学思想的转变，三种模式的演变，代表了人类对音乐本质特征的深刻认识，表征着人类对音乐的本质认识的深度和广度。对此研究对我们今天中国的音乐教育和音乐理论建设有一定的启示作用。     

“互联网+”背景下电子商务在饲料企业经营中的运行机制研究

电子商务的迅猛发展,不仅改变了企业的经营模式,也改变了用户的消费习惯和理念。饲料企业也开始寻求电子商务与传统交易方式相结合的经营模式,谋求企业的长远发展。本文基于"互联网+"背景下,深入分析了我国饲料企业电子商务的经营现状和存在的问题,探讨了电子商务在企业经营中的运行机制和不足之处,并提出了相关的优化对策,以期能帮助企业实现可持续发展。