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以意大利引进Precoce芦笋后代中获得的耐低温、早熟、高产、健壮、抗性强的优质雌性单株A001为试验材料,研究影响其无菌系建立、继代增殖及生根的关键因子,以期为该优质芦笋变异种质在生产和育种中的应用提供支持。结果表明:(1)用75%的乙醇处理30s后,茎尖和茎段分别于2% NaClO溶液中处理8,12min,灭菌率达70%以上;(2) MS+NAA 0.3mg/L+BA 0.2mg/L+KT 0.2mg/L为最佳增殖培养基,增殖系数达4.4;(3)1/2 MS+NAA 0.3mg/L+KT 0.4mg/L+嘧啶醇1.0mg/L为最佳生根培养基,生根率达72.0%。通过嘧啶醇单因子变化试验发现,添加嘧啶醇具有促进芦笋生根的作用;培养基中NAA、BA质量浓度高易引起芦笋玻璃化,加入KT、嘧啶醇后有较好的减轻玻璃化的作用;NAA质量浓度达到0.5mg/L时,对芦笋的生长产生明显抑制作用。 相似文献
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嘧啶醇对马铃薯试管苗生长的影响 总被引:1,自引:0,他引:1
将35个不同基因型的马铃薯试管苗单节段接种在添加20 μmol/L嘧啶醇的MS培养基中,在光照16 h/d和(17±1)℃温度条件下培养6个月后,分析嘧啶醇对试管苗生长的影响.结果显示,除基因型CE76外,20 μmol/L嘧啶醇对于参试基因型试管苗生长速度均有显著的延缓作用,但在基因型间存在较大差异.80%左右的基因型在添加嘧啶醇的培养基中培养6个月后其试管苗株高低于8 cm,其抑制率在50%以上.添加嘧啶醇培养基中培养的试管苗长势健壮,节间距显著缩短而节数没有显著减少,嘧啶醇也强烈地抑制根伸长. 相似文献
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Pre-plant dips of the root portion, shoot portion or entire rooted cutting in ancymidol [α-cyclopropyl-α-(θ-methoxypheny)-5-pyrimidinemethanol] or daminozide [butanedioic acid mono (2,2-dimethylhydrazide)] reduced the height of chrysanthemum (Chrysanthemum × morifolium Ramat. ‘Garland’) regardless of the plant part treated. However, shoot and entire cutting dips were generally more effective than root dips. Height and dry weight decreased with increasing concentrations of both chemicals, and flowering was slightly delayed by ancymidol. Exposure time (length of dip) had no effect on subsequent height reduction. No phytotoxicity resulted from any dip treatment, although in one experiment, daminozide foliar sprays induced a change in flower color. 相似文献
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