全文获取类型
收费全文 | 2395篇 |
免费 | 123篇 |
国内免费 | 233篇 |
专业分类
林业 | 147篇 |
农学 | 184篇 |
基础科学 | 117篇 |
241篇 | |
综合类 | 964篇 |
农作物 | 123篇 |
水产渔业 | 120篇 |
畜牧兽医 | 600篇 |
园艺 | 149篇 |
植物保护 | 106篇 |
出版年
2024年 | 19篇 |
2023年 | 30篇 |
2022年 | 60篇 |
2021年 | 63篇 |
2020年 | 66篇 |
2019年 | 66篇 |
2018年 | 43篇 |
2017年 | 68篇 |
2016年 | 96篇 |
2015年 | 77篇 |
2014年 | 109篇 |
2013年 | 155篇 |
2012年 | 170篇 |
2011年 | 166篇 |
2010年 | 129篇 |
2009年 | 145篇 |
2008年 | 134篇 |
2007年 | 183篇 |
2006年 | 141篇 |
2005年 | 114篇 |
2004年 | 93篇 |
2003年 | 84篇 |
2002年 | 79篇 |
2001年 | 66篇 |
2000年 | 58篇 |
1999年 | 52篇 |
1998年 | 47篇 |
1997年 | 33篇 |
1996年 | 43篇 |
1995年 | 38篇 |
1994年 | 23篇 |
1993年 | 20篇 |
1992年 | 19篇 |
1991年 | 15篇 |
1990年 | 13篇 |
1989年 | 5篇 |
1988年 | 12篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1983年 | 3篇 |
1980年 | 3篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1963年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2751条查询结果,搜索用时 15 毫秒
1.
To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one. 相似文献
2.
3.
猪肺炎支原体及其生物学研究进展 总被引:10,自引:0,他引:10
猪肺炎支原体是引起猪支气管肺炎的主要病原 ,严重危害着养猪事业的发展。虽然猪肺炎支原体的结构简单 ,但由于它对营养要求较高 ,故培养难度大 ,也是人们不能对其深入研究的一个主要原因。近年来利用克隆技术 ,对猪肺炎支原体从基因水平上进行了多种研究 ,已经取得了一些可喜成果。文章从它的生物学特性、荚膜结构、膜蛋白研究以及基因水平的研究等方面进行了综合性论述 ,并简明地指出了目前研究中存在的问题以及未来展望 相似文献
4.
磷对奶牛红细胞膜脂质的影响 总被引:5,自引:0,他引:5
对15例临床上有明显低磷血症的奶牛血清磷、红细胞膜脂质成分进行了测定。结果表明,低磷血症奶牛血清磷、红细胞膜磷脂明显降低;红细胞膜胆固醇及膜胆固醇/磷脂摩尔比值(nch/npl)明显升高,与健康对照组牛相比差异极显著(P<0.01);直线相关分析表明,血清磷与红细胞膜磷脂之间呈极显著正相关(r=0.917,y=10.852x+3.196,P<0.01);与红细胞膜胆固醇以及nch/npl之间分别呈显著负相关(r=-0.940,y=2.850x-1.072,P<0.01;r=-0.920,y=1.968x-1.401,P<0.01)。此外,红细胞膜微粘度与膜磷脂之间呈显著负相关(r=-0.954,y=19.122x-4.384,P<0.01),而与膜胆固醇以及nch/npl之间呈显著正相关(r=0.988,y=0.927x+0.964,P<0.01;r=0.978,y=-0.293x+1.113,P<0.01)。结果表明,磷是红细胞膜脂质成分改变的先决因素,而后者又是红细胞膜流动性及膜结构、功能发生变化并导致膜损伤的一个重要因素。 相似文献
5.
本文根据具体机器动作协调性要求及其具体空间几何条件,采用了摆动从动件盘形凸轮机构。其摆杆推程在O°~52°及52°~62°范围内分别采用了等加速——等速合理拼接运动规律。回程在62°~153°范围内采用了正弦加速度运动规律。用极坐标矢量分析法建立方程、微分,通过电脑程序计算,设计制造出凸轮轮廓,用于禾苗移植机的擒纵机构处,完全符合生产要求,获得了理想的效果。 相似文献
6.
本工作用光镜和电镜技术对全沟硬蜱的围食膜进行了初步观察研究。结果表明,饥饿幼虫、若虫、成虫以及吸血期雄性成虫不具围食膜。吸血期幼虫、若虫及雌性成虫最晚分别于吸血开始后20、20及18小时出现了单层结构的围食膜;缓慢吸血期围食膜的厚度持续增加,而在快速吸血期其厚度却会显著减小。饱血后发育期围食膜不断增厚,愈来愈拱曲,与肠壁的距离不断扩大,并形成多层结构;至少分别于饱血后12、25及11天,仍能在幼虫、若虫及雌性成虫肠腔观察到完好状态的围食膜。全沟硬蜱是我国北方地区多种血液传播病原体的重要媒介,其围食膜的发现及围食膜形态变化的初步描述,对进一步认识这些病原体在全沟硬蜱体内迁移扩散规律、发育动态以及向宿主的传播途径,可提供有益的启示。 相似文献
7.
棉花黄萎病菌毒素结合位点初探 总被引:6,自引:1,他引:5
以纯化的棉花黄萎病菌毒素(PLPC)免疫新西兰白兔制备了PLPC特异性抗血清。将PLPC(15μg·ml 1)用不同浓度的抗体(1~20μg·ml 1IgG)吸附后处理泗棉3号的切根苗,毒素所引起的症状都有不同程度的减轻。表明所制备的抗体在与毒素发生特异性免疫学反应的同时,可部分封闭毒素分子上与毒素受体结合的位点。利用竞争ELISA测定了泗棉3号幼苗子叶的质膜制剂与PLPC的结合活性。结果表明,质膜制剂与毒素结合后能部分阻断毒素与其抗体的免疫学反应,即质膜制剂中含有毒素的结合位点。分别用胰蛋白酶和煮沸处理质膜制剂后,质膜制剂对毒素与其抗体的反应的抑制作用消失,初步表明质膜制剂中与毒素结合的是蛋白质。 相似文献
8.
Field studies were undertaken to explore the weed management strategies for transplanted and direct wet-seeded rice in the Dera Ismail Khan district of North-west Frontier Province, Pakistan. The experimental design was a randomized complete block with a split plot arrangement. The main plots consisted of two planting techniques (transplantation and direct seeding), while weed control practises assigned to the subplots included the use of the granular herbicide Sunstar 15WG (ethoxy sulfuron), Machete 60EC (butachlor), conventional hand weeding, and the weedy check (untreated control). Data were recorded on weed dynamics and the agronomic parameters of the rice crop. Economic analyses on the data were also run. The weed density and biomass were lower in the transplantation plots than the direct-seeding plots. The herbicides ethoxy sulfuron and butachlor reduced the density over the weedy check, with a density comparable to hand weeding in 2002, but slightly higher in 2003. As a result of weed management by hand weeding and herbicides, the paddy yield and its components were significantly higher for the transplanted method compared to the direct-seeded method. The comparable yield of herbicides with hand weeding offers an option for the use of herbicides as an alternative management tool. In light of our findings, it is concluded that for good economic returns, rice crops may be transplanted rather than direct-seeded. The herbicides ethoxy sulfuron and butachlor offer a weed control cover comparable with hand weeding under the transplantation method, while butachlor is good for controlling weeds even under the direct-seeded conditions in the agroclimatic conditions of the area. 相似文献
9.
Pushpanjali R.L. Prasad S.K. Singh S.B. Jadhao 《Pesticide biochemistry and physiology》2005,82(2):103-114
There is a lack of laboratory-based embryonic chicken toxicity studies with the ecologically relevant low dose/s of endosulfan that utilizes a more practical approach such as the chorioallontoic membrane (CAM) injection. In this investigation, 2μg AR grade α-endosulfan/egg (40% of LD50 for embryos) was injected through the CAM in 12-day-old chicken embryos and the activities of glucose-6-phosphatase (G6Pase, EC 3.1.3.9), fructose 1,6-diphosphatase (FDPase, EC 3.1.3.11), adenosine triphosphatase (ATPase, EC 3.6.1.3) and succinic dehydrogenase (SDH, EC 1.3.99.1) and DNA and RNA content in liver and brain tissues and acetyl cholinesterase (AChE, EC 3.1.1.7) in the latter were determined at 24, 48, and 72 h post-exposure. The wet weight of the embryos did not differ between groups. Following endosulfan exposure, except increase in the hepatic ATPase activity (P < 0.01), there was a significant decrease in the following parameters: G6Pase activity in both the liver and brain (P < 0.01), SDH activity in the brain (P < 0.01), brain overall DNA and RNA concentration (P < 0.05), brain AChE activity (P < 0.01). Exposure of 18-day-old embryos to 2-μg endosulfan for 24 h caused decrease (P < 0.01) in the lymphocyte count and IgG content. Histopathology of thymus and bursa of Fabricius revealed a reduction in the population of thymic follicles, smaller thymocytes with the clear vacuoles in cytoplasm and fewer bursocytes accompanied by infiltration of erythrocytes in lymphoid follicles of the endosulfan-treated embryos. It was inferred that in ovo injection of 0.041 μg/g egg weight of α-endosulfan suppress gluconeogenesis (main energy source in embryonic life), nerve transmission, and immunity. 相似文献
10.
Takeshi SHINOGI Tomoko SUZUKI Yoshihiro NARUSAKA Pyoyun PARK 《Journal of General Plant Pathology》2002,68(1):38-45
The rapid generation of reactive oxygen species (ROS), called the oxidative burst, is one of the earliest host responses to
pathogen infection or elicitor treatments. Therefore, we looked for the induction of ROS generation in Japanese pear leaves
by the host-specific toxin, AK-toxin I using a cytochemical method for detecting H2O2. A small amount of non-specific generation of H2O2 was found in the cell walls in toxin- and water-treated susceptible and resistant leaves. Thus, the generation of H2O2 at cell walls appears to be caused by wounding stress during sampling. Specific generation of ROS, however, was found only
in the membrane fragments and extended desmotubules characteristic of modified sites of the plasma membrane in the toxin-treated
susceptible leaves. In addition, generation of H2O2 at plasma membranes was observed with higher frequency in toxin-treated susceptible leaves. This result indicates that the
H2O2 generation was associated with damaged sites in the plasmalemma after toxin treatment and perhaps with the formation of membrane
fragments from altered portions of the invaginated plasma membrane.
Received 21 September 2001/ Accepted in revised form 25 October 2001 相似文献