棉花正反交组合F1代性状的比较研究

按不完全双列杂交,以美国抗虫棉品种A1～A6为父本,国内常规棉品种B7～B12为母本,得到正交组合36个;以B系列为父本,A系列为母本,得到反交组合36个。2002年在湖南长沙、宁乡、南县、澧县4个实验点进行组合F1代产量、纤维品质、抗虫性等性状的正反交比较试验。结果表明:棉花纤维品质在正反交间无差异,棉花纤维品质性状遗传为非细胞质遗传;无论是子棉产量还是皮棉产量,抗虫杂交棉正、反交F1代间存在极显著差异,而且在构成产量的各个因子中,铃重在正反交间的差异达到了显著水平;从抗虫性方面看,正交组合的抗虫性稍好,其中对四代棉铃虫的抗性,正反交间差异达极显著水平。     

芋优异种质资源鉴定评价研究

对农业行业标准《农作物种质资源鉴定评价规范芋》编制的技术路线进行了分析研究,以期为芋优异种质资源的鉴定评价提供参考。     

洱源丛枝瑚杀虫活性成分研究

洱源丛枝瑚(Ramaria eryuanensis)石油醚萃取物经过柱层析得到单体化合物B8,该化合物对小菜蛾3龄幼虫72 h的LC50为3.375 7 mg/mL。经FAB-MS、1HNMR、13CNMR及DEPT等波谱鉴定,并与文献值对照,确定其为麦角甾-7,22-二烯-3β,5α,6β-三醇。     

副溶血性弧菌PCR检测方法的建立

为了建立适于基层单位应用的针对副溶血性弧菌检测的特异PCR方法,检测副溶血性弧菌的基因序列.针对该菌的属特异性基因t1基因进行引物设计,扩增片段大小为449 bp.运用该PCR法可特异性的从副溶血性弧菌ATCC17802标准株中扩增出目的基因片段,与GenBank上发表的序列同源性为100%.而经常污染海产品的溶藻弧菌,嗜水气单胞茵标准株J-1,铜绿假单胞茵标准株ATCC27853结果均为阴性.该PCR法最低检出菌体DNA量为10-2ng以及最低检出菌数为5.7 × 103 CFU/mL.对临床上送检的35份样品进行菌体DNA PCR方法检测并同时与国标GB/T4789.7-2003中使用的检测方法进行比对,两种检测方法的结果完全符合,与国标中使用的检测方法相比可大大缩短鉴定时间.因此该PCR法能快速鉴定当前副溶血性弧菌流行群,有利于流行病学溯源等研究.     

玉米新品种浚单22不同密度对产量的影响

为探讨玉米新品种浚单22获得高产的适宜种植密度，河南省浚县农业科学研究所对浚单22进行了5个处理的密度试验。试验结果表明：在5个试验处理的栽培密度中，以6．00万株／hm^2产量最高，其次为6．75万株／hm^2和5．25万株／hm^2，适宜种植密度为5．25万～6．75万株／hm^2。     

Synthesis and Anthelmintic Activity of 7-Hydroxy- 5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thiocarboxanilides

7-Hydroxy-5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thio-carboxanilides represent a novel series of anthelmintic compounds, with broad-spectrum activity against important parasitic nematodes in sheep and dogs. In particular, an improved efficacy against Trichostrongylus colubriformis in sheep over the related 3-carbamoyl-4-hydroxycoumarins has been noted. New synthetic routes to the key intermediate, 7-hydroxythieno[3,2-b]pyran-5-one, have been developed.     

A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea

The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a ¹³C-enriched isotopic marker, ¹³CO₂, for 24 h during the exponential growth of the algae. The ¹³C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in ¹³C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in ¹³C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the ¹³C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis.     

转Xa21基因杂交水稻选育和评价

通过基因枪转基因方法和双质粒共转化体系将Xa21基因转入优良恢复系明恢63,得到转基因系M12和M22,并进一步做田间试验、新品种选育和食用安全性评价。结果显示:M12和M22对中国的所有白叶枯病小种都表现出高度抗性,但与原受体品种明恢63相比,农艺性状上有许多变异,主要表现在:结实率、千粒重等农艺性状变差,与珍汕97A的配合力显著降低;但与温敏不育系(X07S、056S)配组的F1有较好的田间表现。通过多代回交和分子标记辅助选择,成功地将Xa21基因从M12株系转到保持系80-4B和不育系80-4A中,得到抗白叶枯病的皖21B和皖21A。并利用皖21A不育系选育出优良杂交组合抗优97(皖21A×R-18),该组合在两年区域试验和一年生产试验中产量表现突出,米质优良。对不同世代和不同遗传背景的转基因品系进行白叶枯病鉴定和Southern分析表明:Xa21基因都能稳定遗传和正常表达,连续16代的种植并没使其白叶枯病的抗性减弱或丧失,而且不论Xa21基因是纯合的还是杂合的都有相同的抗性表现。对大鼠和小鼠的饲喂试验表明:转基因大米实质上等同于非转基因大米,是安全无害的。     

白蜡树属树种的园林应用探讨

目前对城市环境的要求越来越高,而提高环境质量的诸因素中,搞好园林绿化是最直接最有效的措施。构成园林绿化的主要材料是园林植物,其主体是树木。作为1个城市,当然是园林树木品种越多,越能构成丰富多彩的园林空间。但是由于地域、气候、科技、经济与自然因素和人为因素的制约,园林树木品种的利用也受到了限制。园林树种的选择不仅要适合本土生长,还要形成地方特色和风格。     

高产抗病大豆新品种绥农22的选育及窄行密植栽培技术

绥农22是黑龙江省农科院绥化农科所经有性杂交育成的高产抗病大豆新品种.于2005年2月经黑龙江省农作物品种审定委员会审定推广.该品种茎秆强硬抗倒,抗病,节短荚密,通风透光性好.蛋白质含量39.66%,脂肪含量20.06%.生育日数118 d左右,需活动积温2 400℃左右.2000年所内鉴定试验平均产量3 894.7 kg/hm2,比合丰25增产17.7%.2004年全省6点生产试验平均产量2 426.1 kg/hm2,比对照品种绥农14增产12%.适应黑龙江省第二积温带广大地区种植,以及吉林省和内蒙古自治区相适应的积温区种植.适宜5月上旬播种,该品种最适宜垄作窄行密植栽培,适宜密度保苗30～35万株/hm2,施种肥200 kg/hm2左右.