臭氧和紫外线对冰箱消毒的研究

家用冰箱存在着不同程度的细菌污染，必需对冰箱进行定期或不定期的消毒。经用载体法和平皿法研究发现，用紫外线对冰箱进行消毒是可行的，其对大肠杆菌、金黄色葡萄球菌和枯草杆菌黑色变种芽胞有较强的杀灭作用，而且不会引起冰箱内温度升高。同时还可产生少量Ｑ，除去冰箱内异味，因此具有杀菌、抑菌、除臭保鲜的作用。     

微波电路电测及其计算方法

从实际工程的角度出发，对电路电测方法进行了论述。在微通信工程中，需要获得障碍物的位置和高度，最合理的天线高度组合和障碍物绕射损耗等参数，为此论述了微波电路电测方法及技术要求。在大庆－秦皇岛输油管道通信工程中采用了Ａ，Ｂ测试法进行测试，得到高度－损耗曲线。这种曲线给出相对自由空间接收电平与收，发天线高度之间的关系。在测试所得的高度－损耗曲线与断面图相结合的基础上，由绕射损耗计算公式推出了几种常见障碍     

Rapid Screening for Aluminum Tolerance in Cereals by Use of the Chlorophyll Fluorescence Test

The effects of aluminum on the photosynthetic apparatus were examined in cereals grown in nutrient solutions (pH 4.5) at two Al levels (0 and 148 μM). The chlorophyll fluorescence kinetics results confirmed that the soft wheat ‘BHG’ cultivar has the potential for growth on acid soils while triticale cultivars ‘Niovi’ and ‘Dada’ appeared to be relatively tolerant. The percentage decrease in F_v/F_m of the less tolerant cultivars after Al-treatment indicated a decrease in the efficiency of the primary photochemistry of PS II, while the decrease in the ratio F_V/F_osuggested that exposure of the cultivars ‘Dio’ and ‘Appulo E’ to aluminum caused injury to the thylakoid structure. The percentage fluctuations of the ratio F_v/F_mwere shown to correlate very closely with the assessment of injury as evaluated by the relative top fresh weight. Measurements of chlorophyll fluorescence in vivo could be used to monitor injury caused by “Al-stress”, and thus they may serve as a rapid screening test for Al tolerance.     

自由曲面前照灯近光配光的防眩目研究

从驾驶员心理学和生理学角度探讨了汽车夜间会车时眩目的机理．由于传统的前照灯配光设计停留在二维的层面上，设计周期长，且配光效果差，很难达到防眩目的要求．本文通过Reflector CAD软件反求出了符合ECE法规配光要求的自由曲面前照灯反射镜的三维模型，该模型可以直接用于数控程序的编制，减小了前照灯的设计成本和设计周期，利用TracePro软件对模型进行近光配光的仿真实验，通过光线追踪的方法避免了实际配光实验时调整反射镜配光所需的反复制造过程，同时又保证了前照灯防眩目的效果。     

Food intake and energy expenditure in growing cats with and without a predisposition to overweight

Overweight and obesity are multifactorial diseases caused by an imbalance in energy metabolism. An underlying genetic predisposition is often a factor in these conditions. In the cat breeding family of the Institute of Animal Nutrition at the Vetsuisse Faculty, University of Zurich, a segregating overweight phenotype with a genetic contribution was observed. From this breeding family, 26 kittens were followed from birth up to 8 months of age. During this time, food intake was measured using an automatic feeding station, and energy expenditure was investigated using indirect calorimetry at the ages of 4 and 6 months. Dual‐energy X‐ray absorptiometry (DEXA) was performed and blood glucose, leptin and insulin were measured at the ages of 4, 6 and 8 months. The kittens were also weighed daily for the first 2 weeks of life, every second day until weaning and once per week until 8 months of age. The body condition score (BCS) was evaluated monthly between 2 and 8 months of age. The main finding of this study is that a predisposition to overweight is connected to a higher food intake early in life, with no significant alterations in energy expenditure. The leptin blood levels were related to body fat percentage, and insulin sensitivity did not seem to be affected.     

X射线荧光光谱法快速测定稻谷中的镉

探索了一种对稻谷中重金属镉的快速、直接的测定方法,以适用稻谷集中收购、存储和流通过程中快速检测的的需要。样品无需前处理,直接磨成粉状即可上机测定。该方法采用X射线荧光光谱分析的原理,样品中的待测元素受激产生特征X射线,并由高性能探测器接收,得到镉元素的信号强度,然后根据信号强度与浓度的比例关系,经过软件处理自动计算样品中镉的含量,20min内完成精确定量。检出限可达到0.034mg/kg,实物标样标准偏差为0.016。此方法简单快速,并且准确性高,重现性好,可广泛应用于稻谷中镉的快速检测。     

Pedotransfer functions to assess adsorbed phosphate using iron oxide content and magnetic susceptibility in an Oxisol

Adsorbed phosphate in soils can be chemically extracted; however, this process is both time‐consuming and not cost‐effective if large numbers of samples have to be analysed. Indirect assessment of adsorbed phosphate by pedotransfer functions (PTFs) can help optimize fertilizer strategies. This study aimed to evaluate the spatial variability of adsorbed phosphate (P_ads), iron oxides and magnetic susceptibility (MS) in oxisols and to calibrate PTFs to predict P_ads. A total of 308 soil samples were collected from Hapludox and Eutrudox soils formed from sandstone in Brazil. The contents of clay (196–607 g/kg), iron oxides (40–165 g/kg), MS (1.2–29 × 10^?6 m³/kg) and P_ads (327–842 mg/kg) were in the range of typical values for these highly weathered soils. This study showed that the attributes studied were spatially dependent. Geomorphic surfaces enabled understanding of spatial variability and helped to develop a more efficient sampling scheme to calibrate PTFs. Moreover, the adsorbed phosphate in these oxisols could be predicted by a PTF using iron oxides and MS as predictors. The MS attribute enabled the most accurate prediction (concordance coefficient = 0.95, root‐mean‐square error = 46 mg/kg and relative improvement in root‐mean‐square error = ?4.12) of spatial variability through PTF compared to other predictors.     

New technique of sex preselection for increasing female ratio in boar sperm model

In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.     

Sequence and unique phylogeny of G genes of bovine respiratory syncytial viruses circulating in Japan

Bovine respiratory syncytial virus (BRSV) is an etiologic agent of bovine respiratory disease. The rapid evolutionary rate of BRSV contributes to genetic and antigenic heterogeneity of field strains and causes occasional vaccine failure. We conducted molecular epidemiologic characterization of BRSV circulating in Japan to obtain genetic information for vaccine-based disease control. Phylogenetic analysis of G and F gene sequences revealed that all of the isolated Japanese BRSV strains clustered in the same genetic subgroup, which was distinct from the 9 known groups. We assigned the Japanese group to subgenotype X. The Japanese isolates formed 2 temporal clusters: isolates from 2003 to 2005 clustered in lineage A; isolates from 2017 to 2019 formed lineage B. The alignment of the deduced amino acid sequences of the G gene revealed that the central hydrophobic region responsible for viral antigenicity is conserved in all of the isolates; unique amino acid mutations were found mainly in mucin-like regions. Our results suggest that BRSV has evolved uniquely in Japan to form the new subgenotype X; the antigenic homogeneity of the viruses within this group is inferred.     

盐胁迫下5-磺基水杨酸、水杨酸对小黑麦种子生理特性的影响

采用种子萌发试验,研究了5-磺基水杨酸、水杨酸对盐胁迫下小黑麦种子萌发及幼苗生长情况的影响.结果表明:在盐胁迫下,适当浓度的两种酚酸均能提高小黑麦种子萌发的发芽率、发芽指数、活力指数,浓度增高则抑制种子萌发.适当浓度的两种酚酸还能降低幼苗叶片电导率和丙二醛(MDA)含量、提高叶绿素含量.总的看来,5-磺基水杨酸的效果比水杨酸好,且低浓度的效果比高浓度的好.