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排序方式: 共有566条查询结果,搜索用时 15 毫秒
1.
Rapid Screening for Aluminum Tolerance in Cereals by Use of the Chlorophyll Fluorescence Test 总被引:1,自引:0,他引:1
The effects of aluminum on the photosynthetic apparatus were examined in cereals grown in nutrient solutions (pH 4.5) at two Al levels (0 and 148 μM). The chlorophyll fluorescence kinetics results confirmed that the soft wheat ‘BHG’ cultivar has the potential for growth on acid soils while triticale cultivars ‘Niovi’ and ‘Dada’ appeared to be relatively tolerant. The percentage decrease in Fv/Fm of the less tolerant cultivars after Al-treatment indicated a decrease in the efficiency of the primary photochemistry of PS II, while the decrease in the ratio FV/Fosuggested that exposure of the cultivars ‘Dio’ and ‘Appulo E’ to aluminum caused injury to the thylakoid structure. The percentage fluctuations of the ratio Fv/Fmwere shown to correlate very closely with the assessment of injury as evaluated by the relative top fresh weight. Measurements of chlorophyll fluorescence in vivo could be used to monitor injury caused by “Al-stress”, and thus they may serve as a rapid screening test for Al tolerance. 相似文献
2.
Food intake and energy expenditure in growing cats with and without a predisposition to overweight
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Vivien Ghielmetti Brigitta Wichert Simon Rüegg Diana Frey Annette Liesegang 《Journal of animal physiology and animal nutrition》2018,102(5):1401-1410
Overweight and obesity are multifactorial diseases caused by an imbalance in energy metabolism. An underlying genetic predisposition is often a factor in these conditions. In the cat breeding family of the Institute of Animal Nutrition at the Vetsuisse Faculty, University of Zurich, a segregating overweight phenotype with a genetic contribution was observed. From this breeding family, 26 kittens were followed from birth up to 8 months of age. During this time, food intake was measured using an automatic feeding station, and energy expenditure was investigated using indirect calorimetry at the ages of 4 and 6 months. Dual‐energy X‐ray absorptiometry (DEXA) was performed and blood glucose, leptin and insulin were measured at the ages of 4, 6 and 8 months. The kittens were also weighed daily for the first 2 weeks of life, every second day until weaning and once per week until 8 months of age. The body condition score (BCS) was evaluated monthly between 2 and 8 months of age. The main finding of this study is that a predisposition to overweight is connected to a higher food intake early in life, with no significant alterations in energy expenditure. The leptin blood levels were related to body fat percentage, and insulin sensitivity did not seem to be affected. 相似文献
3.
探索了一种对稻谷中重金属镉的快速、直接的测定方法,以适用稻谷集中收购、存储和流通过程中快速检测的的需要。样品无需前处理,直接磨成粉状即可上机测定。该方法采用X射线荧光光谱分析的原理,样品中的待测元素受激产生特征X射线,并由高性能探测器接收,得到镉元素的信号强度,然后根据信号强度与浓度的比例关系,经过软件处理自动计算样品中镉的含量,20min内完成精确定量。检出限可达到0.034mg/kg,实物标样标准偏差为0.016。此方法简单快速,并且准确性高,重现性好,可广泛应用于稻谷中镉的快速检测。 相似文献
4.
Pedotransfer functions to assess adsorbed phosphate using iron oxide content and magnetic susceptibility in an Oxisol
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![点击此处可从《Soil Use and Management》网站下载免费的PDF全文](/ch/ext_images/free.gif)
L. A. Camargo J. Marques Jr. G. T. Pereira L. R. F. Alleoni A. S. R. de S. Bahia D. De B. Teixeira 《Soil Use and Management》2016,32(2):172-182
Adsorbed phosphate in soils can be chemically extracted; however, this process is both time‐consuming and not cost‐effective if large numbers of samples have to be analysed. Indirect assessment of adsorbed phosphate by pedotransfer functions (PTFs) can help optimize fertilizer strategies. This study aimed to evaluate the spatial variability of adsorbed phosphate (Pads), iron oxides and magnetic susceptibility (MS) in oxisols and to calibrate PTFs to predict Pads. A total of 308 soil samples were collected from Hapludox and Eutrudox soils formed from sandstone in Brazil. The contents of clay (196–607 g/kg), iron oxides (40–165 g/kg), MS (1.2–29 × 10?6 m3/kg) and Pads (327–842 mg/kg) were in the range of typical values for these highly weathered soils. This study showed that the attributes studied were spatially dependent. Geomorphic surfaces enabled understanding of spatial variability and helped to develop a more efficient sampling scheme to calibrate PTFs. Moreover, the adsorbed phosphate in these oxisols could be predicted by a PTF using iron oxides and MS as predictors. The MS attribute enabled the most accurate prediction (concordance coefficient = 0.95, root‐mean‐square error = 46 mg/kg and relative improvement in root‐mean‐square error = ?4.12) of spatial variability through PTF compared to other predictors. 相似文献
5.
以柑橘溃疡病菌特异性高的假定蛋白基因(登录号:AE008923.1)为靶标,设计并筛选4条引物来特异性识别靶标序列中的6个独立区域,建立柑橘溃疡病菌LAMP实时浊度检测方法。该方法利用实时浊度仪检测反应过程中产生的焦磷酸镁白色沉淀,实现对LAMP整个反应过程的实时监控。从镁离子浓度、甜菜碱浓度、dNTPs浓度、引物浓度和反应温度5个变量参数对 LAMP检测体系进行优化,并对方法的特异性、灵敏度、稳定性及实际样品检测效果进行评价。结果显示,该体系经优化后稳定性良好,可在66 ℃ 恒温条件下,60 min内完成检测;DNA检测下限可达0.02 ng/μL,灵敏度与荧光定量PCR方法相同,比常规PCR方法高1个数量级;能快速、准确地对田间疑似样品进行检测,与荧光定量PCR方法的检出情况一致,没有漏检。结果说明柑橘溃疡病菌实时浊度LAMP检测方法操作简便、所需时间短,特异性与灵敏度高,为柑橘溃疡病菌的快速筛查提供较好的途径。 相似文献
6.
Yoo-Jin Park Ki-Jin Kwon Won-Hee Song Won-Ki Pang Do-Yeal Ryu Md Saidur Rahman Myung-Geol Pang 《Reproduction in domestic animals》2021,56(2):333-341
In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods. 相似文献
7.
Asuka Kumagai Kyoko Kawauchi Kiyohiko Andoh Shinichi Hatama 《Journal of veterinary diagnostic investigation》2021,33(1):162
Bovine respiratory syncytial virus (BRSV) is an etiologic agent of bovine respiratory disease. The rapid evolutionary rate of BRSV contributes to genetic and antigenic heterogeneity of field strains and causes occasional vaccine failure. We conducted molecular epidemiologic characterization of BRSV circulating in Japan to obtain genetic information for vaccine-based disease control. Phylogenetic analysis of G and F gene sequences revealed that all of the isolated Japanese BRSV strains clustered in the same genetic subgroup, which was distinct from the 9 known groups. We assigned the Japanese group to subgenotype X. The Japanese isolates formed 2 temporal clusters: isolates from 2003 to 2005 clustered in lineage A; isolates from 2017 to 2019 formed lineage B. The alignment of the deduced amino acid sequences of the G gene revealed that the central hydrophobic region responsible for viral antigenicity is conserved in all of the isolates; unique amino acid mutations were found mainly in mucin-like regions. Our results suggest that BRSV has evolved uniquely in Japan to form the new subgenotype X; the antigenic homogeneity of the viruses within this group is inferred. 相似文献
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