Novel reassortant H5N6 highly pathogenic influenza A viruses in Vietnamese quail outbreaks

Avian influenza A H5N6 virus is a highly contagious infectious agent that affects domestic poultry and humans in South Asian countries. Vietnam may be an evolutionary hotspot for influenza viruses and therefore could serve as a source of pandemic strains. In 2015, two novel reassortant H5N6 influenza viruses designated as A/quail/Vietnam/CVVI01/2015 and A/quail/Vietnam/CVVI03/2015 were isolated from dead quails during avian influenza outbreaks in central Vietnam, and the whole genome sequences were analyzed. The genetic analysis indicated that hemagglutinin, neuraminidase, and polymerase basic protein 2 genes of the two H5N6 viruses are most closely related to an H5N2 virus (A/chicken/Zhejiang/727079/2014) and H10N6 virus (A/chicken/Jiangxi/12782/2014) from China and an H6N6 virus (A/duck/Yamagata/061004/2014) from Japan. The HA gene of the isolates belongs to clade 2.3.4.4, which caused human fatalities in China during 2014–2016. The five other internal genes showed high identity to an H5N2 virus (A/chicken/Heilongjiang/S7/2014) from China. A whole-genome phylogenetic analysis revealed that these two outbreak strains are novel H6N6-like PB2 gene reassortants that are most closely related to influenza virus strain A/environment/Guangdong/ZS558/2015, which was detected in a live poultry market in China. This report describes the first detection of novel H5N6 reassortants in poultry during an outbreak as well as genetic characterization of these strains to better understand the antigenic evolution of influenza viruses.     

SPME/GC-MS法分析不同龙眼品种果实中的香气成分

采用 SPME/GC-MS法对4个龙眼品种果实香气成分进行了分析鉴定。结果表明，4个龙眼品种共检测出44种芳香物质，其中烷类11种，烯类18种，酯类10种，醇类3种，酮类1种，炔类1种，它们构成4种龙眼主要的香气成分。4个龙眼品种在香气组成和含量上有所差异，储良、东良、东丰、石硖中分别含有25种、24种、26种、21 种香气成分。其中，4种龙眼共有的香气成分有7种：分别是十七烷、罗勒烯(顺式)、罗勒烯(反式)、别罗勒烯、1,3,8-对-薄荷三烯、α-石竹烯、(E)-β-金合欢烯，但其相对含量都有所差异；此外，各品种也具有自己独特的香气成分，如储良特有的香气成分有9种：包括十六烷、2-甲基-4-亚甲基-5 -(2,2-二甲基环丙基)-1-戊烯、5-环丙基戊酸乙酯、二十酸乙酯、棕榈酸乙酯、9-十六碳烯酸乙酯、香叶基芳樟醇、芳樟醇、2,7-二甲基-3-辛烯-5-炔；东丰特有的3种：包括1-碘十一烷、反式，反式-法尼基酸甲酯、2-羟基十二烷酸甲酯；石硖特有的4种：包括十一烷、(-)-异丁香烯、1,3,3-三甲基-2-乙基环己烯、喇叭茶醇；东良没有特有的香气成分。     

A morphological analysis of the skull size and shape of Kerivoulinae (Chiroptera: Vespertilionidae) from Vietnam

Southeast Asia is a region of high biodiversity, containing species of plants and animals that are yet to be discovered. In this region, bats of the subfamily Kerivoulinae are widespread and diverse with six species recorded in Vietnam. However, the taxonomy of the Kerivoulinae in Asia is complicated. In our study, we used diagnostic characters and multivariate analysis to determine morphological differences between the genera Kerivoula and Phoniscus. We showed that the two genera are distinguishable by the size of second upper incisors, the shape of skull, nasal sinus, canines, second upper and lower premolars. In addition, the two genera can be osteometrically separated by measurements of the braincase height, interorbital width and shape of anterior palatal emargination. Our data clearly revealed the morphological variations in the skull shape of Kerivoula hardwickii in Vietnam. This suggests a possible separation into three morphotypes, representing cryptic species supported by statistical differences with wide variation in skull shape, size and teeth. These results demonstrated Kerivoula hardwickii can be separated three subspecies, and the result will serve as the basis for the future assessment and classification of this group in Southeast Asia.     

输液滴灌对龙眼生长及土壤和果实的影响

以石硖龙眼树为试材,对其进行管道输液(CK)、管道输液+地表滴灌(T1)、管道输液+地下10cm滴灌(T2)、管道输液+地下20cm滴灌(T3)、管道输液+地表多孔滴灌(T4)处理,调查其对树体生长,土壤状况和果实产量和质量的影响。结果表明:30d后,T1、T2、T3、T4叶片SPAD值增加量分别比CK提高37.5%、100%、125%、187.5%;T4的枝梢增长量最大,梢长和梢粗分别比CK提高128.34%和110.81%;不同处理在0~100cm土层,土壤含水量大致在15%~35%波动;土壤全氮、全磷和全钾主要集中0~40cm土层;T4果实横径、纵径、单果重、单株产量、可食率和可溶性固形物均比其他处理高,比CK分别提高5.45%、2.58%、8.64%、34.31%、2.85%及26.39%。     

分阶段SO2缓释剂处理结合冰温对龙眼贮藏效果的影响

以'石硖'龙眼Dimocarpus longan Lour.'Shixia'为材料,研究了分阶段SO2缓释剂处理结合冰温贮藏技术对龙眼果实的贮藏效果.结果表明:与3 ℃对照果实相比,分阶段SO2缓释剂结合冰温贮藏技术提高了果皮的h值,改善了果皮色泽,降低了果实呼吸强度,显著抑制了果皮PPO活性,提高了果皮细胞膜透性,贮藏48 d好果率为91.15%,较好地维持了果肉品质和维生素C(Vc)、可滴定酸(TA)和可溶性固形物(TSS)含量,与直接SO2缓释剂结合冰温贮藏技术相比,该技术显著降低了果肉SO2残留量.     

施用蛋鸡粪的龙眼园土壤质量现状评价

本文调查了蛋鸡养殖场粪便产生量及处理利用现状等基本资料,选点采集养殖场配套龙眼园的土壤样品,通过分析测定有机质、全氮、全磷、铜、锌等指标,探讨了施用蛋鸡粪对龙眼园土壤质量的影响,并对龙眼园土壤环境现状进行评价。结果表明,该养殖场龙眼园土壤粪便当量负荷为19.14t·hm-2,未超过以氮计的土壤粪便当量负荷警戒值。四个季节施用蛋鸡粪的龙眼园土壤中有机质、全氮、全磷、铜、锌等指标均高于未施用蛋鸡粪的橡胶园土壤,但无显著差异;施用过蛋鸡粪的龙眼园土壤有机质、全氮、全磷、铜、锌全年平均含量分别为18.71g·kg-1、0.97g·kg-1、0.79g·kg-1、19.65mg·kg-1和43.05mg·kg-1,均显著高于未施用过蛋鸡粪的橡胶园土壤。四个季节龙眼园土壤和橡胶园土壤铜、锌单项污染指数均小于1,属于安全级别。由于龙眼园施用的蛋鸡粪仅占养殖场蛋鸡粪产生总量的2%,而且采用直接施用鲜鸡粪的方式,因此仍存在环境污染的隐患。本文所得结果可为评估蛋鸡养殖场环境污染风险及与果园结合的蛋鸡养殖模式的合理性提供依据。     

缺镁胁迫对龙眼苗期氮代谢的影响

采用溶液培养方法研究了缺镁胁迫对龙眼苗期氮代谢的影响。结果表明 ,缺镁胁迫下 ,龙眼叶片硝酸还原酶 (NR)活性下降 ,游离氨基酸总量增加 ,水解氨基酸总量减少 ,蛋白质合成受阻而分解加剧 ;核酸酶 (RNase)活性提高 ,核糖核酸 (RNA)、脱氧核糖核酸 (DNA)含量降低 ,缺镁对RNA的影响比DNA大 ;活性氧 (O2-)净产生速率增加。统计分析表明 ,缺镁胁迫下 ,龙眼核酸和蛋白质含量下降是其合成受阻、分解加剧以及活性氧毒害综合作用的结果     

龙眼干果与鲜果的糖组分分析

利用高效液相色谱法 (HPLC) 分析了石硖、储良、东壁、青壳宝圆、冬宝9号、公妈本、九月乌、施冲浦、晚香等9个龙眼品种干果和鲜果的糖组分含量。结果表明,9个龙眼品种干果和鲜果中的可溶性糖主要有果糖、葡萄糖和蔗糖;鲜果中的果糖含量16.4 ~ 36.0 mg/g,葡萄糖含量14.9 ~ 33.7 mg/g,蔗糖含量87.5 ~ 161.7 mg/g,总糖含量135.7 ~ 193.0 mg/g,甜度值144.5 ~ 200.8;干果中的果糖含量84.5 ~ 160.7 mg/g、葡萄糖含量67.5 ~ 125.8 mg/g、蔗糖含量169.0 ~ 356.8 mg/g、总糖含量444.6 ~ 538.2 mg/g、甜度值497.2 ~ 590.7;龙眼干果和鲜果中均以蔗糖含量最高;与鲜果相比,干果中的果糖、葡萄糖占比提高,蔗糖占比降低。     

龙荔嫁接龙眼、荔枝实验初报

文章目的是利用龙荔耐寒、抗病及适应性强等优势,作为荔枝龙眼育苗的砧木,观察其嫁接亲和性及生长表现,为筛选龙眼、荔枝的矮化、抗旱、耐贫瘠和抗寒砧木打下基础。试验利用一年半生龙荔实生苗作为砧木,分别嫁接龙眼‘石硖’和荔枝品种‘贵妃红’,对嫁接成活率,接穗品种生长势以及开花挂果等进行调查。结果表明,荔枝嫁接在龙荔砧木上,未有一株存活;龙眼嫁接在龙荔砧木上,成活率为38.57%,而嫁接成活的植株第二年开花结果的植株率有11株,占存活株数的40.74%,说明龙荔作为砧木,与龙眼的亲和性较好,具有作为龙眼育苗砧木的可能。     

越南植物分布新记录

报道了越南2种植物分布新记录,包括夹竹桃科Apocynaceae的保亭鳝藤Anodendron howii Tsiang和大戟科Euphorbiaceae的枝翅珠子木Phyllanthodendron dunniarmm Levl.