向大豆导入几丁质酶基因的初步研究

以根癌农杆菌的Ｔｉ质粒为介导,将抗真菌病的几丁质酶基因,导入默经江省栽培大豆一东农３７号,吉林２８号等１４个品种。从子叶节和下胚轴诱导出丛生芽,并再生植株,经ＰＣＲ和ＤＮＡ分子杂交检测,经ＰＣＲ和ＤＮＡ分子杂交检测的１１３株大豆幼苗中,有７株呈阳性反应,证明几丁质酶基因已导入并整合到大豆的基因组中。     

Lω-空间中加强了的ωT-分离性

在Lω-空间中引进加强了的ωTi（i=1,2）-分离性等概念,系统地讨论了这些概念的特征性质,得出它们保持了LF拓扑空间中Ti-分离性的主要结论,进而说明加强了的ωTi（i=1,2）-分离性是L-好的推广.     

钛对草莓产量、品质影响的研究

匈牙利的I·Pais经多年研究后(1983)认为:钛是植物的一种有益元素。因此,笔者开展了钛在草莓上应用的研究,经两年的研究结果表明:对草莓于盛花期喷洒10ppm的钛溶液,可极显著增产(12-18%)。果实中V_c含量可增加7-30%,可溶性固形物的含量也增加,但有机酸的含量却显著减少;并可使其叶片中叶绿素含量显著增加(22-32%);且果实较对照提早3-4天着色,但不同品种的反应稍有不同。     

Ti6Al4V超声振动切削机理研究

加工钛合金Ti6Al4V时,常规切削的性能往往不能满足要求.鉴于此,比较了不同条件下加工Ti6Al4V的切削加工性能,研究超声振动切削钛合金机理.结果表明,积屑瘤增长阶段,随着速度的升高,积屑瘤逐渐变大,但积屑瘤并不会随速度的增加而无限增大,当积屑瘤增大到一个极限值时会随着速度的增加而逐渐减小.与常规切削相比,在积屑瘤...     

The effect of titanium and gallium on photosynthetic rate of algae

The influence of titanium (Ti) and gallium (Ga) on photosynthesis in synchronized algae (Anacystis nidulans) has been investigated. At moderately low concentrations, Ti and Ga enhanced photosynthetic oxygen evolution and a three‐fold increase in fructose‐1, 6‐bisphosphatase activity. In the presence of Ti, growth as determined by dry weight of Anacystis nidulans was increased, but not so influenced by Ga if the cultures were exposed to continuous illumination.     

农杆菌介导的药用植物遗传转化

利用根癌农杆菌、发根农杆菌介导药用植物的遗传转化。对转化过程中转化方法及转化后的鉴定、影响植物遗传转化的因素与次生代谢产物以及转基因药用植物的获得等方面进行综述。     

芹菜愈伤组织转化的研究

芹菜(Apium graveolens L.)外值体能被含Ti质较载体的根癌农杆菌感染,目前已得到转基因的愈伤组织.该载体带有一个嵌合的npt-Ⅱ(新霉素磷酸转移酶Ⅱ)基因和一个胭脂碱合成酶基因.经胭脂碱测定和DNA分子杂交实验表明,外源的卡那霉素抗性基因已导入芹菜细胞.     

论十八世纪中期中英农法演化成就与变革趋势——《知本提纲》与《马力中耕农法》之比较研究

本文分别依据成书于18世纪中英两国的具有代表性农书《知本提纲》(zhiben ti gang)与《马力中耕农法》(The Horse-hoing Husbandry),加以较为详尽的论述对比,来说明当时中英两国农业生产的情况及特点。并证实其前期的水平,是以劳动集约为主侧重种植业的中国,在总体成就上高于英国。而英国的农业在此之前虽已呈现变革的肇端,但尚未完成;至于工业革命是在本世纪后期方始启动仍在进行之中。如仅就农艺学角度来看,18世纪的中国似更先进,实际上英国粮作单产与畜产品供应却都有所增加,其原因则可归之于劳动生产率的提高。在此之后的发展变革趋势,侧重工程技术的英国随即超过仍沿袭精耕细作仅以生物技术为主的中国,而先于中国实现了农业的近代化的历史进程。     

Agrobacterium pusense,a new plant tumour‐inducing pathogen isolated from Lawson cypress

Lawson cypress (Chamaecyparis lawsoniana), an important landscape tree, is widely planted in gardens and parks throughout Iran. Crown gall disease on Lawson cypress trees was observed in Sari and Juybar Counties, Mazandaran province, northern Iran, in 2017. Isolation from galls on potato dextrose agar (PDA) containing CaCO₃ yielded bacterial colonies, the predominant types of which were purified and selected for characterization. The isolates were Gram‐negative, oxidase positive, able to grow in 2% NaCl and produced 3‐ketolactose. They hydrolysed esculin, casein and arbutin but not starch, gelatin or Tween 80. Two representative isolates were selected for PCR amplification and sequencing of DNA gyrase subunit B (gyrB) gene. In the phylogenetic tree based on the partial sequence of the gyrB gene, isolates KH1 and KH2 clustered with Agrobacterium pusense. The pathogenicity of all isolates was confirmed by inoculation on Jimsonweed (Datura stramonium) and carrot discs (Daucus carota). Confirmation of the presence of genes involved in pathogenicity was made by performing PCR with the virD2A/virD2C and VCF/VCR primer pairs which resulted in amplification of the expected 224 and 730 bp fragments in all studied isolates, respectively. A. pusense was therefore identified as the causal agent of crown and stem gall of Lawson cypress. This appears to be the first report on the natural occurrence of crown gall disease on Lawson cypress and the first record of a plant disease caused by A. pusense.     

甜椒Ti+离子注入相关诱变育种效应研究

进行了Ti+离子注入甜椒诱变相关育种效应的研究。结果表明,低能Ti+离子注入后能引起甜椒形态特征的变化,其中在小剂量时表现为改善植物的生长发育状况,促进生长与分枝;在大剂量时表现为突变株增加诱发新变异。另外离子的注入引起叶片中Na、Mg、K、Ca、Si等元素含量下降,Al、Fe的含量上升。