Study on Site-specific Nutrient Management in Cotton Field

The study on the characteristics of spatial variability of soil nutrients and fertilizer recommendations in cotton field under certain conditions of agricultural management was conducted with GIS and systematic approach for soil nutrient constrains. The results showed that of the spatial variability of soil nutrient was greatly related to the management condition of previous crops. Grid sampling and variable rate application technology (VRAT) were the tools that would hopefully increase fertilizer efficiency. The fertilizers were applied where they were needed and at proper rate. Balance fertilization demonstration showed that fertilizer recommendations according to the available nutrient level in soil could decrease fertilizer cost with 657.4 yuan / ha and increase seed cotton yield by 19.8%. A net profit of the balanced fertilization was 5314.9 yuan / ha higher than that of local fertilization practice.     

Interactions between crop biomass and development of foliar diseases in winter wheat and the potential to graduate the fungicide dose according to crop biomass

Foliar pathogens such as Zymoseptoria tritici and Puccinia striiformis causing septoria leaf blotch and yellow rust respectively can cause serious yield reduction in winter wheat production, and control of the diseases often requires several fungicide applications during the growing season. Control is typically carried out using a constant fungicide dose in the entire field although there may be large differences in crop development and biomass across the field. The objective of the study reported in this paper was to test whether the fungicide dose response curve controlling septoria leaf blotch and other foliar diseases in winter wheat was dependent on crop development and biomass level. If such a biomass dependent dose response was found it was further the purpose to evaluate the potential to optimize fungicide inputs in winter wheat crops applying a site-specific crop density dependent fungicide dose. The study was carried out investigating fungicide dose response controlling foliar diseases in winter wheat at three biomass densities obtained growing the crop at three nitrogen levels and using variable seed rates. Further the field experiments included three fungicide dose rates at each biomass level, an untreated control, and 75%, 50% and 33% of the recommended fungicide dose rate and the experiments were replicated for three years. Crop biomass had a significant influence on occurrence of septoria and yellow rust with greater disease severity at increasing crop biomass. In two of three years, the interaction of crop biomass and fungicide dose rate had a significant influence on disease severity indicating a biomass-dependent dose response. The interaction occurred in the two years with high yield potential in combination with severe disease attack. If the variation in crop density and biomass level obtained in the study is representative of the variation found cultivating winter wheat in heterogeneous fields, then there seems to be scope for optimizing fungicide input against foliar diseases site-specific adapting the dose according to crop density/biomass.     

Remote sensing of biotic and abiotic stress for irrigation management of cotton

The applicability of commercially available remote sensing instrumentation was evaluated for site-specific management of abiotic and biotic stress on cotton (Gossypium hirsutum L.) grown under a center pivot low energy precision application (LEPA) irrigation system. This study was conducted in a field where three irrigation regimes (100%, 75%, and 50% ET_c) were imposed on areas of Phymatotrichum (root rot) with the specific objectives to (1) examine commercial remote sensing instrumentation for locating areas showing biotic and abiotic stress symptomology in a cotton field, (2) compare data obtained from commercial aerial infrared photography to that collected by infrared transducers (IRTs) mounted on a center pivot, (3) evaluate canopy temperature changes between irrigation regimes and their relationship to lint yield with IRTs and/or IR photography, and (4) explore the use of deficit irrigation and the use of crop coefficients for irrigation scheduling. Pivot-mounted IRTs and an IR camera were able to differentiate water stress among irrigation regimes. The IR camera distinguished between biotic (root rot) and abiotic (drought) stress with the assistance of groundtruthing. The 50% ET_c regime had significantly higher canopy temperatures than the other two regimes, which was reflected in significantly lower lint yields when compared to the 75% and 100% ET_c regimes. Deficit irrigation down to 75% ET_c had no impact on lint yield, indicating that water savings were possible without reducing yield.     

麦秸还田及氮肥管理技术对水稻产量的影响

以水稻武香粳14和扬粳9538为材,大田设麦秸还田与不还田、不施氮肥、习惯施肥法（FFP）和基于叶绿素仪(SPAD)测定值的氮肥管理(SSNM)等处理。结果表明,SSNM较FFP氮肥施用量减少40.0%~41.7%,而产量平均增加3.81%。秸秆还田的较秸秆未还田的产量平均增加2.49%。氮肥的农学利用率、吸收利用率和生理利用率均以秸秆还田高于秸秆不还田处理。与秸秆未还田或FFP相比,秸秆还田和SSNM降低了生育前期的分蘖数、叶面积指数和干物质积累（但抽穗及以后差异较小）;提高了茎蘖成穗率、结实期叶片光合速率和根系活力。说明秸秆还田和SSNM有利于生育后期群体光合生产和提高物质生产效率。秸秆还田和实地氮肥管理提高了氮收获指数和氮肥利用效率。对秸秆还田和SSNM的产量、生育特性及氮肥利用效率进行了讨论。     

空间变异在土壤性质长期定位观测及取样中的应用

通过对中国科学院沈阳生态实验站30m×42m样地进行网格法分层取样,采用经典统计学与地统计学相结合的方法,以土壤pH和有机碳(TOC)为例,探讨了空间变异在土壤性质定位试验及取样中的应用。结果表明,49对样本的pH和TOC变异系数均小于10%,变量在0~10cm和10~20cm两个层次的基本统计数值基本接近,最佳理论模型的参数显示各变量空间变异主要受结构性因素的影响,且以135°方向的变异为主;相对误差为10%时采集6个和10个土样可分别达到95%和99%的置信水平,结合空间分布格局可以确定更为精确的取样方案;综合分析表明待选样地可以作为土壤性质长期定位观测试验用地。     

一对等位基因互作控制的核雄性不育性

通过遗传工程能够产生由两个基因共同作用而形成的雄性不育。一个可以是能够导致雄性不育的基因,另一个可以是该雄性不育基因的活化基因,它们共同存在的时候表现雄性不育。利用位点特异性重组和转基因技术能够将这两个基因安排在植物同源染色体的相同位置上表达,成为等位基因,而获得分别只具有其中一个基因的两转基因系。它们之间杂交,F1中两个基因同时存在,F1产生雄性不育而成为不育系。常规品系与此F1不育系杂交,在杂种植株中,这两个基因不能同时存在,所有植株育性恢复。利用光温敏核不育性,化学杀雄和人工去雄可解决此不育系繁殖问题。该雄性不育性利用方式优越,育种简便易行,能够满足对最佳组合选育的要求,且能够定向培育目标杂交组合。     

Herbicide savings and economic benefits of several strategies to control Sorghum halepense in maize crops

This study was conducted to assess the herbicide savings and the cost efficiency of site-specific herbicide application strategies in comparison with other strategies based on uniform application of herbicides throughout the whole field. The specific situation considered was Sorghum halepense infested maize fields in Spain. The results from a theoretical economic model were contrasted with the information derived from a S. halepense survey conducted in 37 commercial maize fields distributed over three Spanish maize production areas. Seven application strategies were simulated: 1) no herbicide; 2) overall full-rate; 3) overall half-rate; 4) and 5) site-specific spraying infested cells with full- and half-rate, respectively; 6) and 7) site-specific spraying infested cells plus adjacent buffer areas with full- and half-rate, respectively. The simulation results showed that site-specific weed management was the most profitable strategy when S. halepense infested area ranged between 6.5 and 18.7%. This scenario was present in 22% of the surveyed fields. In fields with less than 6.5% infestation (a situation present in 51% of the surveyed fields), yield losses were slight and the most profitable strategy was using no herbicide. When the infested area ranged between 18.7 and 40.8% (19% of the surveyed fields), no significant differences were observed between the net benefits of the various strategies. Full rate herbicide applications throughout the entire field resulted in the highest net returns in fields with more than 40.8% weed infestation (8% of the surveyed fields). In these cases, the added costs of weed detection, mapping and site-specific herbicide application were not justified by the herbicide savings obtained. Likewise, herbicide savings obtained with the various strategies depended on the proportion of the field infested. Considering the high difference between site-specific treatments and uniform application of herbicides when the infested area ranged between 18.7 and 40.8%, and that no significant differences in net returns were observed in this range, site-specific treatments should be preferred. Adding a buffer area to the site-specific treatments does not seem a suitable strategy due to the considerable increases in costs and reductions in herbicide savings.     

表达新城疫病毒融合蛋白基因禽痘病毒重组体的构建

通过RT-PCR扩增新城疫病毒F基因,利用禽痘病毒复合启动子、SV40 PolyA终止信号序列构建F基因表达盒。将F基因表达盒与loxp-GFP-loxP序列串联插入禽痘病毒重组臂,构建了禽痘病毒转移质粒载体。在脂质体介导下转染CEF,获得了带有绿色荧光信号的重组病毒rFPVFGFP。通过二次转染,利用Cre-loxP位点特异性重组将重组病毒基因组的GFP自动去除,结果获得了只含新城疫病毒F基因表达盒的重组禽痘病毒rFPVF。试验结果表明,rFPVF复制稳定,表达的F蛋白具有免疫原性,能够刺激鸡只产生抗新城疫病毒的中和抗体。     

Patchy weed distribution and site-specific weed control in winter cereals

Site-specific weed control in winter cereals was performed on the same fields every year over a 5-year period (1999–2003). The most common weeds (Apera spica-venti, Galium aparine, Veronica hederifolia, Viola arvensis) were counted by species, at grid points which were georeferenced and the data were analysed spatially. For weed control, weeds were grouped into three classes: grass, broad-leaved weeds (without Galium aparine), and Galium aparine. Based on weed distribution maps generated by the spatial analyses, herbicide application maps were created and site-specific herbicide application was carried out for grouped and or single weed species. This resulted in a significant reduction in herbicide use. Averaging the results for all fields and years, the total field area treated with herbicides was 39% for grass weeds, 44% for broad-leaved weeds (without Galium aparine) and 49% for Galium aparine. Therefore, site-specific weed control has the potential to reduce herbicide use compared to broadcast application, thus giving environmental and economic benefits.     

Maximum benefit of a precise nitrogen application system for wheat

Research is ongoing to develop sensor-based systems to determine crop nitrogen needs. To be economic and to achieve wide adoption, a sensor-based site-specific application system must be sufficiently efficient to overcome both the cost disadvantage of dry and liquid sources of nitrogen relative to applications before planting of anhydrous ammonia and possible losses if weather prevents applications during the growing season. The objective of this study is to determine the expected maximum benefit of a precision N application system for winter wheat that senses and applies N to the growing crop in the spring relative to a uniform system that applies N before planting. An estimate of the maximum benefit would be useful to provide researchers with an upper bound on the cost of delivering an economically viable precision technology. Sixty five site-years of data from two dryland winter wheat nitrogen fertility experiments at experimental stations in the Southern Plains of the U.S.A. were used to estimate the expected returns from both a conventional uniform rate anhydrous ammonia (NH₃) application system before planting and a precise topdressing system to determine the value of the latter. For prices of $0.55 and $0.33 kg⁻¹ N for urea-ammonium nitrate (UAN) and NH₃, respectively, the maximum net value of a system of precise sensor-based nitrogen application for winter wheat was about $22–$31 ha⁻¹ depending upon location and assumptions regarding the existence of a plateau. However, for prices of $1.10 and $0.66 kg⁻¹ N for UAN and NH₃, respectively, the value was approximately $33 ha⁻¹. The benefit of precise N application is sensitive to both the absolute and relative prices of UAN and NH₃.This is journal paper AEJ-260 of the Oklahoma Agricultural Experiment Station, project H-2574.