稀释液渗透压对绵羊精液冷冻效果的影响

本试验对绵羊冻精采用两次稀释时,从调整Ⅱ液中葡萄糖含量和降低甘油浓度两方面,改变稀释液的渗透压,测定冻精效果。先增减糖的比例,配制9-2(对照), 84-1和84-2等试验组,其稀释Ⅱ液渗透压1191,1026、1499mmol/kg,获冻精情期产羔母羊率61.69, 63.31, 54.55(P<0.05)。随后, 以84-1为对照,降低甘油浓度,设3个试验组,即87-2,88-1和88-1a,渗透压相应为747,456,463mmol/kg,冻精情期受胎率1987年84-1,87-2为65.37,67.37;1988年87-2,88-1,88-1a为63.16,62.50,46.55(P<0.05)。试验说明,绵羊冻精采取2次稀释法,第Ⅱ液以4％甘油,渗透压为747mmol/kg时,冻精效果最佳。     

胶孢炭疽菌株QZ-97a分生孢子货架期及对温度的耐受力

将不同含水量的胶孢炭疽菌婆婆纳专化型菌株QZ 97a分生孢子粉置于4℃和室温下,密闭或开放贮存。结果表明,4℃下密闭贮藏6个月,分生孢子萌发率和芽管长度无明显降低,室温贮藏降低也不明显。贮藏10个月后,各种贮藏方式孢子的活力都显著下降。但以4℃、含水量4%、密闭贮藏孢子活力较为稳定,420d孢子萌发率为70 4%,芽管长度为贮藏60d时的43 2%。含水量9 6%、开放室温下贮藏的孢子粉活力下降最大。因此,温度和孢子粉含水量是影响孢子货架期的两个主要因素。分生孢子粉对温度有一定的耐受性,随温度增高,敏感性增强,60℃下分生孢子的活力下降显著,处理6min,孢子芽管长度较对照下降50%。     

浅谈猪精液保存在人工授精中的发展与展望

在世界上主要的猪肉生产国中,猪人工授精(AI)技术的应用率在90%以上。尽管猪冷冻精液技术在不断进步,但由于其效率不高,在猪场实际生产中并不多见使用。在猪人工授精技术中,液态精液是目前应用最广泛的精液,与传统的牛冷冻精液不同,其独特的精子微胶囊保护膜技术,可以对猪精子进行更加完善的液态保存,因此液态精液是目前猪人工授精使用的主要方式。文章综述了猪精液保存技术在人工授精中的应用,并展望了今后的发展方向,为人工授精技术助力恢复生猪生产提供参考。     

早胜牛的采精及细管冻精制作技术要点

近年来，伴随我国畜牧业蓬勃发展的同时，为了更大限度的提高母畜的受胎率和充分利用优秀种畜的基因，人工授精技术得到了不断完善和发展，并在全国各地广泛推广和应用。本文结合实践经验总结出的一些技术措施，阐述了对早胜牛这一地方优良种质资源进行保种和开发利用的重要意义，同时指出人工授精技术和低温冷冻保存精液技术在早胜牛上的实践，对于进一步开发利用培育优质高产肉牛新品种，促进肉牛产业高质量发展具有非常重要的现实意义。通过早胜牛种公牛采精的全过程以及细管冻精制作要点的详细讲解，低温冷冻保存精液技术，可更经济、可靠地实现家畜品种资源的保护，让优良基因长时间保存使得后续研究需要得到保障。     

Quality of Fresh,Cooled, and Frozen Semen From Stallions Supplemented with Antioxidants and Fatty Acids

This study assessed the effect of oral supplementation with the primary antioxidants and fatty acids involved in spermatogenesis (L-carnitine, selenium, vitamin E, omega-3, and omega-6) on the seminal quality in fresh, cooled, and frozen semen of stallions (n = 8), using a randomized design. The animals were divided into Group I (n = 4) and Group II (n = 4) for a 30-week experiment. The two groups alternated between nutraceutical supplementation and a placebo over the course of the experiment. Semen collections were performed in two sets: once in the middle of the experiment, before the two groups switched treatments, and once at the end. The volume, appearance, sperm concentration, spermatozoa kinetics, and membrane integrity of fresh semen were evaluated. The spermatozoa kinetics and membrane integrity of cooled (for 24, 36, and 48 hours) and frozen semen were also evaluated. No differences were observed in volume, appearance, and sperm concentration between treatment and control. However, compared with placebo, nutraceutical supplementation increased (P < .05) total motility, trajectory speed, as well as plasma and acrosomal membrane integrity in spermatozoa from fresh semen. In cooled semen, nutraceutical treatment also increased (P < .05) total motility, speed, and membrane integrity of spermatozoa compared with the control. In frozen semen, supplementation increased (P < .05) spermatozoa progressive motility and plasma membrane integrity. Our results suggest a positive, synergistic effect of the antioxidant L-carnitine and selenium on spermatozoa kinetics. Similarly, the increase in plasma and acrosomal membrane integrity could be attributed to higher concentrations of polyunsaturated fatty acids (a key cell-membrane component), combined with the prevention of excess lipid peroxidation by antioxidants. In conclusion, supplementation with nutraceuticals containing fatty acids and antioxidants improved the quality of fresh, cooled, and frozen stallion semen. Therefore, nutraceutical use should increase the success of artificial insemination with cooled and cryopreserved semen.     

不同LED光质对培养火龙果胚性愈伤组织的影响

以“玫瑰红龙”火龙果成熟胚诱导的愈伤组织为材料,研究不同光质配比的LED光处理对火龙果愈伤组织生长的动态影响。结果表明1红1绿光质配比,12h/d照射,最有利于火龙果愈伤组织的生长,在28d至42d细胞增长最快且活性最高,为0.78-0.75。其次是白光>1红1蓝>1绿1蓝>1红1绿1蓝>红光,在70d培养周期内,第35d是较理想的收获时间。     

GA3不同浓度和施用时期对‘灿烂’兔眼蓝莓花期和生殖能力的影响

本研究以5年生‘灿烂’兔眼蓝莓为试材,以清水为对照,开展 GA₃ 不同浓度和不同施用时期的喷施处理,调查与分析各处理对开花物候期、结果枝比率、平均每枝花朵数、花粉活力、柱头可授性和胚囊活力等参数的影响,以此评价GA₃不同浓度和不同施用时期对蓝莓花期和生殖能力调节的效果,以期探索适用的蓝莓花期延迟技术,为生产栽培花期管理提供科学依据。研究结果表明,GA₃不同浓度和不同时期的处理对‘灿烂’兔眼蓝莓的开花物候期有着明显的影响,随施浓度的增加开花物候期延迟的时间逐渐延长,但同时其结果枝比率、平均每枝的花朵数、花粉活力、柱头可授性和胚囊活力等雌雄器官的生殖能力均逐渐降低。此外,在采果后不同时期第一次喷施500 mg/L GA₃的处理对成花和生殖能力的影响更为显著,整体上呈现为施用时期越早,成花效果越差,雌雄性器官的生殖能力亦越低。综合分析表明,施用GA₃可以延迟其花期7 d左右,但第一次施用时期过早,或施用浓度过高均不同程度地影响蓝莓的成花效果和生殖能力,在采果后第30 d施用500 mg/L的GA₃效果较好。     

花龄对蓝莓柱头可授性及花粉活力的影响

以 南高丛蓝莓‘蓝雨’为试材,研究了花龄对柱头可授性和花粉活力的影响。结果表明：‘蓝雨’蓝莓雌雄性器官的育性具有同步性,有利于自然授粉结实。从花后第1d-5d花粉活力都较高,特别是花后第2d-4d,花粉活力保持在50%以上,是人工辅助授粉花粉的良好收集期;从花后第2d-4d柱头分泌黏液较多,此期柱头具有强可授性,是开展人工辅助授粉的最佳时期。     

Effect of Linc00152 targeting miR-376c-3p on viability,apoptosis and radiosensitivity of cervical cancer cells

AIM: To investigate the effect of Linc00152 on the viability, apoptosis and radiosensitivity of cervical cancer cells. METHODS: RT-qPCR was used to detect the expression levels of Linc00152 and microRNA-376c-3p(miR-376c-3p) in human cervical cancer HeLa cells and SiHa cells, and normal cervical Ect1/E6E7 cells. The cervical cancer HeLa cells with low Linc00152 expression or miR-376c-3p over-expression were established. MTT assay, flow cytometry, colony formation assay and Western blot were used to determine the cell viability, apoptosis, radiosensitivity and related protein expression. The dual-luciferase reporter assay was used to verify the regulatory relationship between Linc00152 and miR-376c-3p in the HeLa cells. RESULTS: Compared with the Ect1/E6E7 cells, Linc00152 was up-regulated in the HeLa cells and SiHa cells, and miR-376c-3p was down-regulated (P < 0.05). Low expression of Linc00152 or over-expression of miR-376c-3p inhibited the viability of HeLa cells, induced apoptosis, enhanced the radiosensitivity, inhibited the protein expression of cyclin D and Bcl-2, and promoted the protein expression of P21 and Bax (P < 0.05). Linc00152 negatively regulated miR-376c-3p expression in the HeLa cells, and inhibition of miR-376c-3p expression reversed the effect of low expression of Linc00152 on HeLa cell viability, apoptosis and radiosensitivity. CONCLUSION: Linc00152 is highly expressed in the cervical cancer cells. Linc00152 affects the viability, apoptosis and radiosensitivity of HeLa cells by targeting miR-376c-3p, which is a potential diagnosis and treatment target for cervical cancer.