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XU Liangmei LI Jianping YAN Changjiang SHAN Anshan 《东北农业大学学报(英文版)》2007,14(4):323-326
The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy(HPLC).The sample was extracted exceedingly in the critical limit of CO 2 .The retention time of Schisandrin A and Schisandrin B was reduced,with methano/water(75:25)as mobile phase.The wavelength for detection was 254 nm. The R2 of standard curve was 0.9998 and the relative standard deviation was 2.31%and 3.17%with the recovery of 96.45%and 97.37%,respectively.The result shows that the rate of veracity of this method is higher and it proves that the determination method of Schisandrin A and Schisandrin B in Schisandra chinensis is a feasible method. 相似文献
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The effects of schisandrin B (Sch B) and its analogs on solar irradiation-induced oxidative injury were examined in BJ human fibroblasts. Sch B and schisandrin C (Sch C) increased cellular reduced glutathione (GSH) level and protected against solar irradiation-induced oxidative injury. The photoprotection was paralleled by decreases in the elastases-type protease activity and matrix-metalloproteinases-1 expression in solar-irradiated fibroblasts. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production. The results suggest that by virtue of its pro-oxidant action and the subsequent glutathione antioxidant response, Sch B or Sch C may offer the prospect of preventing skin photo-aging. 相似文献
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【目的】建立小鼠组织中五味子甲素的质量浓度测定方法,并研究五味子甲素在小鼠体内的组织分布及其靶向治疗效果。【方法】将五味子甲素按25mg/kg一次灌胃ICR雄性小鼠,于给药后0.5,1,2,3和4h分别取小鼠心、肝、肾、脑,用生理盐水洗净后制成0.25g/mL组织匀浆,以睾丸酮为内标物,采用高效液相色谱法(HPLC)测定五味子甲素在小鼠心、肝、肾、脑中的质量浓度,并对HPLC方法的专属性、回收率、精密度和稳定性进行考察。依据五味子甲素在各组织中的峰值及各时间段的质量浓度,确定其分布最高的组织,对质量浓度分布最高的组织进行相关的药理学评价。【结果】小鼠心、肝、肾、脑组织中五味子甲素质量浓度为0.625~20μg/mL时,其质量浓度与五味子甲素/内标峰面积线性关系良好(r≥0.999 0)。HPLC法的专属性良好;日内精密度为(3.15±0.82)%~(4.77±0.57)%,日间精密度为(5.51±1.82)%~(8.46±2.93)%;稳定性试验的RSD10%;绝对回收率为(89.04±3.82)%~(91.37±3.77)%,相对回收率为(95.65±5.76)%~(106.07±8.29)%。五味子甲素1次灌胃给药(剂量25mg/kg)后在小鼠体内心、肝、肾、脑组织中均有分布,给药1h后五味子甲素在小鼠心脏、脑组织中质量浓度达到峰值,给药2h后在小鼠肝脏和肾脏中质量浓度达到峰值。整个分布情况显示,给药后1,2,3h五味子甲素在小鼠肝脏组织中的质量浓度均高于其他组织,且达峰值时其质量浓度也显著高于心脏、肾脏、脑(P0.05);其次是肾脏,五味子甲素质量浓度较低的为心脏和脑。五味子甲素对CCl4所致的小鼠急性肝损伤确有一定的保护作用。【结论】该测定方法简便、快速、稳定,可用于小鼠内脏组织中五味子甲素质量浓度的测定;小鼠灌胃给药后五味子甲素在小鼠体内心、肝、肾、脑组织中均有分布,且达峰值时以肝脏中质量浓度最高,其次是肾脏,心脏与脑中较低;五味子甲素对CCl4所致的小鼠急性肝损伤有一定保护作用。 相似文献
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研究北五味子叶片中五味子醇甲、五味子甲素、五味子乙素含量的日变化,为今后人工合成木脂素类成分和开展优质高效栽培技术研究提供理论依据。超声提取五味子叶片的木质素类成分,并利用HPLC对五味子醇甲、五味子甲素、五味子乙袭进行含量测定。3种木脂素类成分在叶片中的含量从6:00时至9:00时呈上升趋势(五味子醇甲和五味子乙素在9:00时达到1d中的最大值);9:00时至15:00时呈递减趋势;15:00时至18:00时又呈上升趋势(五味子甲素在18:00时达到1d中的最大值).同一节位叶片中木脂素类成分含量的高低顺序为五味子乙素〉五味子醇甲〉五味子甲素。 相似文献
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Philip Y. Lam Po Yee Chiu Hoi Yan Leung Na Chen Pou Kuan Leong Kam Ming Ko 《Fitoterapia》2010,81(8):1239-1245
The effect of schisandrin B (Sch B) on long-term ethanol-induced oxidative stress in various rat tissues was investigated. Long-term ethanol treatment increased reactive oxygen metabolites (ROM) level in plasma. The ethanol-induced oxidative stress was assessed by mitochondrial glutathione and α-tocopherol levels, antioxidant enzyme activities, malondialdehyde (mtMDA) production and heat shock protein (Hsp) 25/70 levels. Liver was most susceptible to oxidative stress with a significant increase in mtMDA production. Long-term Sch B treatment enhanced mitochondrial antioxidant status in a tissue non-specific manner. Sch B co-treatment ameliorated the alterations in plasma ROM levels, mtMDA production and Hsp 25/70 expression in rat tissues. 相似文献
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[目的]探讨五味子甲素、乙素及丙素对人皮肤细胞衰老作用的影响,为开发北五味子抗皮肤衰老产品奠定基础。[方法]通过H2O2造成HaCat细胞氧化损伤衰老模型,采用噻唑蓝(MTT)法测定HaCat细胞增殖情况。[结果]浓度50μmol/L的H2O2对细胞无明显抑制作用,浓度100和200μmol/L组有明显抑制作用。H2O2组细胞的存活率为(71.667±0.862)%。浓度100和200μmol/L五味子甲素组细胞的存活率分别为(76.400±0.656)%和(82.367±0.666)%;浓度100和200μmol/L五味子乙素组细胞的存活率分别为(86.300±0.721)%和(88.900±0.985)%;浓度100和200μmol/L五味子丙素组细胞的存活率分别为(86.767±0.551)%和(88.067±0.873)%。[结论]五味子甲素、乙素及丙素对H2O2造成细胞的氧化损伤均具有一定的保护作用,以五味子乙素和丙素的效果较好。 相似文献
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Schisandrin B (Sch B) and schisandrin C (Sch C), but not schisandrin A and dimethyl diphenyl bicarboxylate, protected rat skin tissue against solar irradiation-induced oxidative injury, as evidenced by a reversal of solar irradiation-induced changes in cellular reduced glutathione and α-tocopherol levels, as well as antioxidant enzyme activities and malondialdehyde production. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production in rat skin microsomes. Taken together, Sch B or Sch C, by virtue of its pro-oxidant action and the subsequent eliciting of a glutathione antioxidant response, may prevent photo-aging of skin. 相似文献
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