落桦混交林幼龄期Ⅰ级木分布规律研究

利用３６块落叶松一样树混交林标准地调查资料，研究其幼龄期林分Ⅰ级木单位面积株数与林分密度之间的关系，结果表明：Ⅰ级木的株数并非随密度的增加而直线递增，而是趋向“二阶常数”分布，且在同一年龄阶段，在较低的密度范围（２３２５～４８００株／ｈｍ２）或较高的密度范围（４８０１～６７５０株／ｈｍ２）．Ⅰ级木的株数变动幅度不太大。     

Analysis of Growth and Productivity of Indian Mustard (Brassica juncea) in Relation to FYM, Nitrogen and Source of Fertilizer

A field experiment was conducted during the winter seasons of 1992–93 and 1993–94 at Anand to study the effect of FYM, nitrogen and source of fertilizer on growth and yield of mustard [ Brassica juncea (L.) Czernj & Cosson]. The results showed significant variation in leaf area index (LAI), crop growth rate (CGR), dry matter production and seed yield. The direct effect of farmyard manure (FYM) was conspicuous in improving the growth of mustard. FYM application at 10 tonnes ha⁻¹ significantly increased the LAI, CGR and dry matter accumulation per plant at almost all the stages during first year study (1992–93) and in pooled analysis. Similarly, nitrogen application registered maximum LAI, CGR at 75 kg level and RGR and NAR at 50 kg level at almost all the during both years. Sulphur carrying source (Ammonium sulphate plus single super phosphate) increased all stages growth characters. Maximum dry matter accumulation per plant and seed yield were recorded with highest levels of FYM (20 tonnes ha⁻¹), N (75 kg ha⁻¹) and source having S. Seed yield was strongly associated with LAI and dry matter accumulation per plant at all the stages.     

玉米小斑病抗性种质鉴定及生理生化分析

以200份玉米优良自交系为材料,2022-2023年夏分别进行小斑病菌的田间接种,综合两年数据共鉴定出高抗小斑病的玉米自交系3份,分别为K22、CML50和齐319;筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明,48 h和72 h时,自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73,进一步验证田间鉴定的准确性。48 h或72 h时,高抗小斑病自交系K22与感病对照B73相比,5种防御酶活性增加14.32%～70.45%。     

30%乙酰甲胺磷乳油对水稻纵卷叶螟的防治效果与技术

对30%乙酰甲胺磷乳油防治水稻纵卷叶螟(Cnaphalocrocis medinalis)的田间效果进行了试验。结果表明:使用30%乙酰甲胺磷乳油防治水稻纵卷叶螟的杀虫效果均在71.7%以上,保叶效果高达83.8%。     

芍药属组间杂交种皮开裂种子的解剖观察与胚培养

【目的】探究芍药属组间杂交种皮开裂种子的内部状态和胚培养成苗表现,为揭示种皮开裂种子的成苗潜力、提高组间远缘杂交育种效率提供参考。【方法】以24个芍药属组间杂交组合种皮开裂的种子为试验材料,解剖后观察并统计胚和胚乳的发育情况,通过离体培养观察胚的萌发和生长规律,分析不同组合种皮开裂种子发育和成苗差异。【结果】芍药属24个组间杂交组合中均出现了种皮开裂的杂交种子,平均种皮开裂率高达61.08%;1 767粒种皮开裂种子中,有11.09%的胚乳已腐烂;胚乳未腐烂种子中,约90%以上种子的胚乳存在不同程度的损伤或病变,24.18%的种子内部未观察到可见胚。对解剖得到的1 185个组间杂种胚进行离体培养发现,有24.73%的胚不萌发,63.47%的胚生长异常,仅有4.47%的胚能正常生长,得到完整植株。胚培养得到的完整植株具备良好的活力,移栽成活率达到86.79%。24个杂交组合种皮开裂种子通过离体胚培养共得到46株移栽成活的组间杂种苗,平均成苗率为2.60%,接近正常组间杂交种子的播种成苗率（2.75%）。【结论】种皮开裂现象在芍药属组间杂交种子中普遍存在,是组间远缘杂交障碍的重要表现之一,用离体胚培养技术能有效克服这类障碍,提高成苗率。     

不同叶面肥对柑橘果实品质及产量的影响

探明不同种类叶面肥对柑橘果实品质及产量的影响,为柑橘提质增效提供理论依据。以柑橘品种“宫川”为试材,设置喷施清水（CK）、磷酸二氢钾（T1）、奇茵（T2）及欧甘（T3）4个处理,比较分析各处理对柑橘果实品质及产量的影响。结果表明,不同水溶肥对果实的单果重、纵径、横径及净重差异不显著。喷施叶面肥后,T1、T2、T3的可溶性固形物含量和可滴定酸含量比CK有所增加,但差异不显著。T2的可溶性固形物含量和可滴定酸含量最高,分别为9.39%、0.75%,显著高于CK,可溶性固形物含量比CK提高了14.93%,可滴定酸含量比CK提高了19.05%。T2、T3、T1处理的单株挂果数分别比CK高31.59%、16.28%、4.46%;T2、T3、T1处理的每公顷产量分别比CK高12.96%、6.57%、3.00%。结论：喷施叶面肥能提高柑橘果实品质和产量,T2的综合效果更好。     

不同烤烟品种烟叶质量对比分析研究

采用田间随机区组试验设计方法结合数学统计分析方法,研究“云烟97”、“云烟121”、“秦烟96”、“NC71”4个烤烟品种烟叶产质量。结果表明：云烟121烟株农艺性状、烟叶外观质量、烟叶化学成分含量最好;4个烤烟品种各等级烟叶总氮含量偏低,总糖、还原糖含量均偏高,烟碱、氯、钾均在优质烟叶适宜范围。     

黄河三角洲重度盐碱地甜高粱品种筛选和配套施肥技术研究[ ]

本研究以中科院科甜系列甜高粱为研究对象,针对重度盐碱地,从种植品种、肥料配比和有机肥类别三个方面进行试验,目的为了探索重度盐碱地甜高粱的种植技术,为黄河三角洲重度盐碱地甜高粱的栽培推广提供理论依据和技术支持。结果表明:（1）种植资源评比试验:在重度盐碱地对科甜13（KT13）、22（KT22）、41（KT41）、42（KT42）、43（KT43）5个甜高粱种质资源进行资源评比,KT41产量最高,达到82.87 t/hm2,KT13其次,产量为66.28 t/hm2。（2）重度盐碱地肥料配比试验:底肥施加300kg/hm2复合肥和15t/ hm2有机肥（H1Y1）、30t/ hm2有机肥（H0Y2）;300kg /hm2复合肥和30t/ hm2有机肥（H1Y2）均能显著提升重度盐碱地甜高粱产量,H0Y2处理产量最高,达到73.16t/hm2,相比于对照增幅达到64%。（3）重度盐碱地有机肥类别试验:施加牛粪、有机肥和海藻肥均能提升重度盐碱地中甜高粱产量,在相同投入成本下,海藻肥处理的产量最高为67.88 t/hm2,相比于对照处理增幅39.8%。综上考虑,在黄河三角洲重度盐碱地可选择KT13和KT41两个耐盐碱资源,底肥中施加15 t/hm2以上的普通有机肥或者牛粪同时配施复合肥,有机肥可选择微生物有效活菌数≥5亿/g的高品质海藻肥替代普通有机肥,进行甜高粱种植能获得较好的产量。     

应用纳米磁珠实时荧光定量PCR方法检测番茄黄化曲叶病毒

In order to develop a rapid, sensitive and specific qPCR assay for detection and quantification of Tomato yellow leaf curl virus (TYLCV), a pair of primers and TaqMan probe were designed according to the conserved sequence of known TYLCV isolates. Combining with MNP technique, a novel MNP-qPCR detection method was established and verified based on specificity, sensitivity and reproducibility tests. The results indicated that the Ct value of plotted standard curve showed good linear relationship(R² =0.9994)with the log of copy number of template. The established method showed a high specificity for TYLCV detection without crossing reaction with Tomato severe leaf curl virus and Tomato yellow leaf curl Sadinia virus, and was 10-fold more sensitive than routine PCR. Both coefficients of variation were less than 2%, indicating a good reproducibility. We have provided a novel method for detection of TYLCV in plant samples rapidly and quantitatively.