Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.     

我国农药残留快速检测技术的研究与应用现状

本文简述了目前农药残留快速检测技术的研究概况，分析了农药残留快速检测技术在我国的实际应用情况。     

Cortical gene expression pattern in rat focal cerebral ischemia

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.     

UHPLC-Q/TOF MS结合HPLC-DAD定性和定量分析硫酸黏菌素可溶性粉中的未知添加物

定性和定量分析一批兽药硫酸黏菌素可溶性粉中的未知添加物。照《中国兽药典》2010年版一部对该批检品用微生物检定法进行含量测定时,发现该样品的抑菌圈为虚圈,用薄层色谱鉴别该样品,未显示与标准品溶液一致的主斑点,怀疑该样品中有处方外非法添加物。采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q/TOF MS)对该样品进行筛查,发现疑似添加物,并使用液相色谱-二极管阵列检测(HPLC-DAD)法进行了双重确证和含量测定。该样品中非法添加物确证为磺胺氯达嗪和甲氧苄啶,添加量分别为58.5 mg/g和13.4 mg/g。本研究通过建立筛查方法为监管部门提供技术支撑,通过分析非法添加物的可能原因为打击兽药处方外非法添加提供了思路。     

Automatic Measuring Method of Eyeglass Lens Power

A method of measuring eyeglass lens power is proposed. A position detector and 3 beams of ray are used to measure the prismatic, spherical and astigmatic powers. Based on the method, an automatic focimeter with simple structure can be developed. The measure errors of prismatic and spherical powers are analyzed, from which it is concluded that the precision of the position detector is the key to the method.     

饲喂机器人的研究与开发

精确饲喂的机器人主要由行走机构、料箱、分料螺旋和控制系统等部分组成,利用霍尔传感器和无线识别装置分别实现自身的精确定位以及奶牛的识别。机器人的控制器采用PIC16F877微处理器构成,主要完成机器人的运动控制以及与管理微机的数据交换。饲喂机器人效率高、成本低,定量饲喂有利于提高奶牛的产奶量,适合在奶牛场推广和应用。     

生产效率视域下加工番茄生产要素冗余的区域差异及影响因素

新疆是我国番茄制品生产和出口的重要基地,基于新疆加工番茄主产县域480份农户的调研数据,采用DEA-BCC模型,测算新疆加工番茄主产县域的生产效率,分析加工番茄主产县域的生产要素冗余率,并利用地理探测器模型,揭示影响加工番茄生产效率的关键因子。结果表明：新疆加工番茄主产县域的生产效率存在显著差异,且生产效率均未达到DEA有效水平;其次,加工番茄主产县域的生产要素投入存在不同程度的冗余,种植面积和农业机械投入冗余属于资源利用强度不足型,劳动力、化肥及农药投入冗余属于要素投入过度型;家庭纯收入、户主年龄、户主受教育程度、种植时间和种植面积是生产效率出现县域差异的主要因子,解释力度介于61.4%～65.2%,且因子间相互作用的影响力均高于单独作用的影响力,表明促进县域之间生产要素的合理流动和高效集聚,发挥主产县域的辐射带动作用,可以有效提高加工番茄种植户的生产效率。     

Transient heat transfer characteristics of air-array-jet impingement with different nozzle arrangements on high-temperature flat plate in small jet-to-plate distance

Numerical studies on transient heat transfer characteristics of air-array-jet impingement with a small jet-to-plate distance and a large temperature difference between nozzles and plate were presented.The dimensionless jet-to-plate distance(H/D)was 0.2,and non-dimensional nozzle-to-nozzle spacing(S/D)was 3,4,5 and 6,respectively.It is found that the quenching time is shortened at a constant total mass flow at air jet inlet m·(m·=218.21 kg/h),and the heat transfer uniformity is deterio-rated as S/D increases.However,the adding reversed-flow nozzles can shorten the quenching time of the glass plate considerably with a modest change in the heat transfer uniformity.The results at variable m·are the same as those at a fixed m·.Furthermore,the parity and arrangement of nozzles are also discussed,It is found that an odd number of nozzles is more beneficial for transient heat transfer.Based on these results,an appropriate proposal for ultra-thin glass tempering process is presented.