Blackgram fiber (Phaseolus mungo): Mechanism of hypoglycemic action

The effect of the administration of blackgram fiber (Phaseolusmungo) on the metabolism of carbohydrates was studied in rats fed 30%NDF (neutral detergent fiber) diet. The experimental group showed a significant increase in liverglycogen level and a significant decrease in blood glucose. Significantincreases in the activities of glycogen phosphorylase, hexokinase, fructose-1,6-diphosphatase, glucose-6-phosphate dehydrogenase and isocitratedehydrogenase were observed in the experimental group. The activities ofphosphoglucomutase and glucose-6-phosphatase were significantly lower inrats fed the fiber diet. The study showed that blackgram fiber exhibitssignificant hypoglycemic action in experimental animals.     

大白菜葡萄糖磷酸变位酶同工酶的纯化及其分子性质

首先对影响葡萄糖磷酸变位酶（ＰＧＭ）稳定性的因素进行了比较研究,结果发现,在所有被检测的因子中,磷根根对酶保护作用最强,而Ｔｒｉｓ－直向效果极差。在磷酸缓部液中添加二硫苏糖醇、ＰＭＳＦ、葡萄糖和镁离子等可能的保持试剂未能进一步提高酶的稳定性。另外,ＰＧＭ酶的稳定性有较大影响。酸性ＰＨ不利于酶活性的保持,在ＰＨ８．０时酶最为稳定,因此,本实验对ＰＧ册工酶的纯化均在ＰＨ８．０的磷酸缓冲液中进行。依次经     

微生物磷酸葡萄糖变位酶的研究进展

从磷酸葡萄糖变位酶对碳代谢和细菌细胞形态的影响、细菌致病性、在细菌高效产氢中的作用、细菌脂多糖生物合成中的作用和维持酵母细胞内Ca2+平衡的作用等方面,介绍了微生物磷酸葡萄糖变位酶的研究进展。     

磷酸变位酶遗传表现与结球白菜耐热性的关系

用结球白菜耐热和热敏感品种,研究了种苗中磷酸变位酶（ＰＧＭ）同工酶表现形式与耐热性的关系。发现ＰＧＭ－２位点带型的表现形式与耐热性相关。用耐热与不耐热品种杂交后的Ｆ１、Ｆ２和Ｆ３代株系分析了ＰＧＭ－２位点与耐热性的相关性,结果表明,ＰＧＭ－２位点可以作为结球白菜的一个耐热标记位点。