Effect of Synthetic D,L-Lactide with PD PDiluent

To discuss the effect about yield of synthetic D,L-lactide with potassium dihyrogen phosphate(PDP) diluents.The optimization process of synthetic D,L-lactide was selected by contrast experiments,optimization experiments and selective experiments of PD Pdiluents quantity,then D,L-lactide produced was analyzed by qualitative analysis and quantitative analysis.The yield of D,L-lactide was improved from 25.65% to about 40.00% when PD Pdiluents that was the most effective factor to affect yield was used from 4%to 6%,however,the rudimental PD Pcontent was only 0.012% and the content of D,L-lactide was above 99.40%.The yield of D,L-lactide was improved effectively with PD Pdiluents,and the D,L-lactide,which structure wasnot changed,could be polymerized into PLA and other polymers for enough content.     

小麦雄性不育系中TaPDC-E1a及其调节酶基因的表达特征

为进一步研究杀雄剂SQ-1诱导小麦雄性不育的机制,采用电子克隆的方法分离TaPDC-E1a基因,并利用半定量RT-PCR技术分析该基因及其调节酶基因PDK和PDP的表达特性。结果表明,TaPDC-E1a基因编码388个氨基酸,具有TPP保守结构域,可能存在2个丝氨酸磷酸化位点;与可育系相比,TaPDC-E1a基因在生理型不育系和遗传型不育系中表达下调;PDK基因在生理型不育系中表达下调,而在遗传型不育系中表达上调;PDP基因在可育系及不育系中的表达趋势无明显变化。表明经杀雄剂SQ-1诱导形成的生理型不育系在败育过程中其能量代谢途径更容易受到影响,推测对PDK基因进行调节的上游信号机制在小麦生理型不育系与遗传型不育系中可能不一致。