渗透调节物质在植物抗寒基因工程的应用

提高农作物的抗寒性具有重要的现实意义,文中综述了渗透调节物质对提高植物抗寒能力的作用原理以及有关基因导入植物的研究进展,包括导入甜菜碱基因、脯氨酸基因、海藻糖相关基因等,并指出了研究中存在的问题及可能的解决策略。     

Transformation of Brassica oleracea var. capitata with bacterial betA gene enhances tolerance to salt stress

The bacterial betA gene for biosynthesis of glycinebetaine was transferred to cabbage (Brassica oleracea var. capitata) cultivar ‘Golden Acre’ through Agrobacterium-mediated transformation of hypocotyl explants. Transgenic status was established through Southern hybridization and mRNA expression in the shoots. The transformants exhibited higher tolerance to NaCl stress compared to untransformed parent plants. In physiological assessment of salinity tolerance, transgenics showed better growth response and greater stability in maintaining plant water relations at increasing levels of salinity. These results demonstrate that engineering glycinebetaine biosynthetic pathway into cabbage can lead to enhanced salt tolerance.