Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.     

A review of models of landscape change

Models of landscape change may serve a variety of purposes, from exploring the interaction of natural processes to evaluating proposed management treatments. These models can be categorized as either whole landscape models, distributional landscape models, or spatial landscape models, depending on the amount of detail included in the models. Distributional models, while widely used, exclude spatial detail important for most landscape ecological research. Spatial models require substantial data, now more readily available, via remote sensing, and more easily manipulated, in geographical information systems. In spite of these technical advances, spatial modelling is poorly developed, largely because landscape change itself is poorly understood.To facilitate further development of landscape models I suggest (1) empirical multivariate studies of landscape change, (2) modelling of individual landscape processes, (3) explicit study of the effect of model scale on model behavior, and (4) scaling-up results of studies, on smaller land areas, that have landscape relevance.     

舒城县油茶低产林类型与综合改造技术模式

舒城县是安徽省油茶重点发展县,油茶产业开发走在全省的前列。本文阐述了舒城县现有油茶低产林类型、改造模式及其技术措施,对分类实施油茶低改具有一定现实意义和指导意义。     

浅谈推算和预测宏观尺度土壤N_2O释放量的方法

土壤是N2 O的最主要释放源 .本文综述了根据局部地区土壤N2 O释放观察结果 ,推算和预测宏观尺度土壤甚至全球土壤N2 O释放通量变化和年释放量的方法 ,评述了各类方法的优缺点 ;并着重讨论了释放过程机理模型的应用 .     

利用析因法测定产蛋鸡必需氨基酸需要量、模式及模型

本研究是3期饲养试验内容的概括和总结，目的是使所建立的估测产蛋鸡必需氨基酸（EAA）需要模型具有广泛的适应性和准确性。对394只罗曼蛋鸡的试验数据进行多元回归分析。根据食入的每个EAA（主要包括赖氨酸、蛋氨酸、色氨酸、苏氨酸、亮氨酸、异亮氨酸、苯丙氨酸、缬氨酸、组氨酸、精氨酸、胱氨酸、酪氨酸）与产蛋、维持和增量所需的相应EAA的回归关系，测定相应的回归系数，建立了估测每个EAA需要的数学模型，可估测产蛋鸡在任何生产条件下每个EAA的需要量及模式。另外．本试验还建立了指数曲线模型（R2为0．71），唐它来描述食入EAA与产蛋量间的关系比较恰当，不过，用来估测产蛋鸡EAA需要量时，其估测值与实测值的吻合度没有桥因法好（R2为0．80）。     

南美白对虾双茬淡化养殖技术研究

研究采用南美白对虾塑料大棚保温三级淡化暂养技术等措施,实现南美白对虾池塘双茬淡化养殖。养成期间水质情况良好,水温平均（26±3）℃,pH值约8．5左右,溶氧量5．6m叽以上,氧氮约0．20mg/L,亚硝酸氮0．02m班左右,每667m^2养殖产量达426．7kg,养殖效益高达4186．7元。结果表明,采用南美白对虾双茬淡化养殖技术,延长了对虾生长期,提高了池塘利用率和经济效益,具有较好的推广价值.